ISSN:8755-1039    

DOI:10.1002/dc.2840080303

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractBecause they present marked cellular changes, the cytological appearances of epithelial repair may be confused with those of cancer. To see whether the Ag‐NOR staining technique for nucleolar organizer regions would be useful to distinguish benign proliferative reactions (squamous metaplasia and repair) from cervical intra‐epithelial neoplasia (CIN I, CIN II, and CIN III), we studied a series of cervical smears. The smears, previously stained with the Papanicolaou technique, were destained and restained with Ag‐NOR silver. Significantly different (P<0.05) Ag‐NOR counts were found in squamous metaplasia, epithelial repair, and various degrees of CIN. The Ag‐NOR technique appears useful in the diagnosis of cervical cytology and particularly in cases with marked cellular change, which could be confused with neoplasia. © 1992 Wiley

ISSN:8755-1039    

DOI:10.1002/dc.2840080304

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractOver a period of 2 yr (1987‐1988), FNA smears in 574 cases were found to have cytologic features suggestive of or consistent with tuberculous lesions. The age of the patients ranged from 6 mo to 75 ye, with a median of 24 yr. The male to female ratio was 273:301. Sites of FNA were superficial lymph nodes (SLN) in 440 (76.7 percent) cases, superficial extranodal sites (SENS) in 50 (8.7 percent), both SLN and SENS in 7 (1.2%), the thoracic cavity in 16 (2.8%), and the abdominal cavity in 61 (10.6%). Cytologic features were described under 3 major cytologic patterns, i.e., type I: epithelioid granuloma without necrosis, type II: epithelioid granuloma with necrosis, type III: necrosis without epithelioid granuloma. Type I, II, and III reactions were observed in 181 (31.5%), 183 (31.9%), and 210 (36.6%) cases, respectively. The overall AFB positivity was 30.8%. The AFB positivities for type I, II, and III cytologic reactions were 5.4%, 32.0%, and 48.5%, respectively. The AFB positivity was tow (60.0%) was observed in lesions of the thorax (63.6%) and thyroid (62.5%). © 1992 Wiley‐Liss,

ISSN:8755-1039    

DOI:10.1002/dc.2840080305

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractSince cyclophosphamide and busulphan used for therapy of bone marrow transplantation (BMT) can cause urothelial cell changes similar to those found in bladder cancer, comparative morphologic and morphometric urinary cytologic research was carried out, examining 812 urine samples taken from 121 patients undergoing BMT and 60 urine samples from 20 patients with bladder cancer. The morphological results showed some differences in the characteristics of the urinary sediment in urothelial toxicity caused by conditioning therapy in BMT and in bladder cancer; among these were background, cellularity, leukocytes, urothelial cell arrangement, cell shape and size, vacuolization, mitosis, and nucleoli. A comparative morphometric study was also carried out, showing differences regarding cell area, nuclear area and perimeter, and N/C ratio, especially between well‐differentiated bladder cancer and urothelial toxicity. © 1992 Wiley‐Liss,

ISSN:8755-1039    

DOI:10.1002/dc.2840080306

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractDyskeratotic cells that occur in squamous cervical epithelium under certain abnormal conditions appear in cervical smears in sheets or aggregates, or as miniature cells, either isolated or phagocytosed. In order to determine the tissue correlates of such cells, 116 cervical biopsies with a histopathologic diagnosis of papillomavirus (HPV) infection were reviewed and compared to previous findings in cytological smears; the patients were 18‐65 yr of age. Eighty of the 116 cases had, in addition, cervical intra‐epithelial neoplasia (CIN). A much higher number of miniature dyskeratotic cells was recorded in the biopsies from patients with both HPV and CIN types of lesions (76.3% of the biopsies had isolated miniature cells and 41.3% showed phagocytosed miniature cells), compared to those with only HPV infection (19.4% with isolated and 5.5% with phagocytosed miniature cells). In addition, in the HPV plus CIN patients, the more advanced the neoplastic lesion, the higher the percentage of cases in which both types of dyskeratocytes are observed. These highly keratinized isolated miniature cells are found in, and are considered to arise from, the deeper layers of the epithelium; when phagocytosed they appear in the center of a concentric arrangement of cells. These cells constitute a pathological entity that can be distinguished from the sheets and aggregates of dyskeratotic cells that come from the superficial layers of parakeratotic epithelia. © 1992 Wiley‐Lis

ISSN:8755-1039    

DOI:10.1002/dc.2840080307

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractTo evaluate the supportive role of image cytometry and DNA ploidy pattern in the diagnosis of thyroid tumors, a preliminary study was performed on fine needle aspirates of 30 cases. Of these, 10 cases each were of colloid goiter, follicular neoplasm, and papillary carcinoma. The nuclear area and DNA value of 50 cells in each case were measured. The mean nuclear area in colloid goiter (69.50 + 12.62 sq.μm) was significantly lower than the mean nuclear area in a follicular neoplasm (88.71 + 15.51 sq.μm) (P<0.05). Similar differences between the mean nuclear area in colloid goiter and papillary carcinoma (124.0 + 12.27 sq μm) was also highly significant (P<0.001). The results obtained by image cytometry were compared with estimated DNA ploidy pattern of follicular cells from the same cases. All colloid goiter had mean nuclear area below 100sq.μm with diploid DNA value. However, papillary carcinoma showed aneuploid DNA pattern in eight cases (80.0%), but mean nuclear area was above 100 sq. μm. A diagnostically useful finding obtained in two of the 10 cases of follicular neoplasm was the association of aneuploid DNA pattern with mean nuclear area of the follicular cells above 100 sq.μm. indicating a high probability of carcinoma and thus demanding an urgent open biopsy. These cases were readily distinguished from other cases of the same category showing diploid DNA pattern and mean nuclear area of follicular cells below 100 sq.μm. © 1992 Wiley‐

ISSN:8755-1039    

DOI:10.1002/dc.2840080308

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractAcanthamoeba species infect humans occasionally and act as opportunistic pathogens in immunocompromised individuals. This study demonstrates the application of cytocentrifugation as an aid to identification of Acanthamoebae. In addition, certain staining procedures clearly optimized visualization of characteristic amoebic features. This was demonstrated by adding amoebae from laboratory cultures to bronchoalveolar lavage specimens. In preparations stained by the Papanicolaou, trichrome, or hematoxylin‐eosin (H&E) procedures, the discrete deeper staining nucleolus was the most distinctive feature. The vacuolated cytoplasm also aided in the identification of amoebae. These features were less apparent and often distorted following staining of Acanthamoeba species with the Hema III and Giemsa procedures. © 1992 Wiley‐Liss,

ISSN:8755-1039    

DOI:10.1002/dc.2840080309

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractThree spindle cell neoplasms were encountered in a series of 46 FNA of the adrenal performed between 1984 and 1991. These neoplasms included a recurrent undifferentiated adrenal cortical carcinoma (ACC) with a predominant spindle cell pattern, a pheochromocytoma (PC), and a metastatic desmoplastic malignant melanoma (DMM), Cytologically, the ACC was characterized by the presence of numerous microtissue fragments composed of spindle‐shaped malignant cells with oval to spindle‐shaped nuclei, one or more nucleoli, and bipolar cytoplasmic processes. In some areas the tumor cells were dissected by vascular channels. The background contained abundant metachromatic stroma as well as individually scattered tumor cells. The PC was composed predominantly of loosely cohesive spindle‐shaped cells along with more polygonal shaped cells with delicate faintly staining granular cytoplasm. The tumor cells exhibited mild anisonucleosis. The tumor fragments were well vascularized by arborizing delicate capillary channels. The DMM was composed of microtissue fragments, interlacing fascicles and loose aggregates of spindle‐shaped malignant cells with hyperchromatic nuclei, small nucleoli, and an absence of cytoplasmic pigment. In each case ancillary studies including immunocytochemistry and electron microscopy (EM) were helpful in the differential diagnosis. The ACC was negative for cytokeratins, neuron‐specific enolase (NSE), and muscle‐specific actin (HHF), but displayed strong positivity for vimentin as welt as characteristic whorls of smooth endoplasmic reticulum by EM. The PC was positive for NSE and chromogranin with no EM performed. The DMM stained for S‐100 and vimentin but was negative for HMB‐45. cytokeratin, and HHF. EM examination revealed rare atypical premelanosomes. A discussion of spindle cell lesions of the adrenal gland encountered in FNA biopsy is presented along with the role of ancillary studies in the work‐up of these cases. © 19

ISSN:8755-1039    

DOI:10.1002/dc.2840080310

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractFive cases of Ki‐1‐positive anaplastic large‐cell lymphoma diagnosed by fine‐needle aspiration biopsy are reviewed, and cytologic, histologic, and ultrastructural findings in these cases are correlated. In all cases, the diagnosis of anaplastic large‐cell lymphoma was suggested on the basis of the morphological appearance in aspiration smears. This diagnosis was confirmed by immunohistochemistry, which revealed strong positivity of most of the cells by Ki‐1 antibody. Two of the lymphomas were T‐cell type, one was B‐cell type, and the remaining 2 were composed of null cells. In 2 cases, intracytoplasmic inclusions were seen in some of the tumor cells in aspiration smears. These were ultrastructurally correlated with large lysosomal bodies of variable morphology. Fine‐needle aspiration combined with immunohistochemistry may be an effective technique for diagnosing this neoplasm. © 19

ISSN:8755-1039    

DOI:10.1002/dc.2840080311

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY

摘要:

AbstractWe have studied 3 cases of sarcoidosis involving the parotid gland by means of fine‐needle aspiration cytology (FNAC). The main findings were noncaseating granulomas, multinucleated giant cells (MGCs), and lymphocytes. In one case MGCs contained asteroid bodies and in another case we observed calcium oxalate crystals (COCs) over both stromal fragments and MGCs. Although nonpathognomonic for sarcoidosis, these 2 findings may help in the diagnosis of this condition. However, both are easily overlooked and must be borne in mind when viewing noncaseating granulomas. Sarcoid granulomas displaying COC must be differentiated from foreign‐body granulomas. The aforementioned cytological findings must be assessed in conjunction with clinical findings. Nevertheless, in most cases the diagnosis of sarcoidosis is made by exclusion. © 1992 Wiley‐Lis

ISSN:8755-1039    

DOI:10.1002/dc.2840080312

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1992

数据来源: WILEY