ISSN:8755-1039    

DOI:10.1002/dc.2840110202

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1994

数据来源: WILEY

摘要:

AbstractImmunohistochemical analysis of p53 has become an established method of detecting mutated p53. Immunolocalization of p53 has been extensively studied in tissue sections of many human neoplasms, but not in cytology specimens. Therefore, we performed Immunolocalization of p53 in both cytology and tissue preparations obtained from the same specimens in 19 cases of human esophageal squamous‐cell carcinoma. Three different antibodies, PAb1801, DO‐7, and CM‐1, were employed. Positive p53 nuclear immunoreactivity was observed in carcinoma cells, but not in noncarcinomatous cells, in both tissue and cytology preparations. The incidence of p53 positivity was 53% (10/19) in cytology and 42% (8/19) in tissue, and the coincident rate of both results was 89% (17/19). We conclude that immunohistochemistry of p53 in cytology is a useful method for detecting p53 mutations, and may contribute to the diagnosis of malignancy, because it is a simple, easy, and rapid technique. Diagn Cytopathol 1994; 11:113–118. © 1994 Wiley

ISSN:8755-1039    

DOI:10.1002/dc.2840110203

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1994

数据来源: WILEY

摘要:

AbstractFine‐needle aspiration cytology and clinical presentations of 16 cases of plasma cell dyscrasias (PCD) have been studied in a period of 6 yr. There were seven cases of solitary plasmacytomas of bones, two cases of extramedullary plasmacytomas, and seven cases of multiple myelomas. Differential counts of mature and immature plasma cells along with presence of cell clumps, mitosis, and bi/multinucleations were studied. The cytological findings revealed no correlation with the different subtypes of PCDs; however, it was helpful in the differential diagnosis. Bone narrowing findings, immunological parameters, available histopathology, and X‐ray appearances were analysed. Clinical diagnosis of PCDs were made in only three cases. Thus fine‐needle aspiration biopsy was considered as an important diagnostic modality in tissue diagnosis of plasma cell tumors, particularly in solitary plasmacytoma of bone and extramedullary plasmacytoma. Diagn Cytopathol 1994; 11:119–123. © 1994 Wiley

ISSN:8755-1039    

DOI:10.1002/dc.2840110204

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1994

数据来源: WILEY

摘要:

AbstractAlthough histologic grading of breast carcinoma is widely practiced by most pathologists, cytologic grading of fine‐needle aspirates (FNA) of this neoplasm is not commonly done. This study addresses the issue of the accuracy of a new classification system, a simplified Nuclear Grading (NG) system based on the criteria proposed by Black et al. (Surg Gynecol Obstet 1955; 100:543) in FNA of breast carcinoma. We reviewed 100 cases of breast carcinoma, initially diagnosed by fine‐needle aspiration biopsy (FNAB) with subsequent histologic confirmation, consisting of 94 ductal, five lobular, and one medullary carcinoma. NG of Papanicolaou's stained materials were reviewed twice independently by two pathologists and then were compared to the original histologic NG. The concordance rate with histology ranged from 80–90%. Intraobserver reproducibility was 86 and 88%, while interobserver reproducibility ranged from 84–88%. Of the 88 cases with corresponding flow cytometic (FCM) data, there were 35 diploid and 53 aneuploid cases. Fifty‐nine (95%) of histologic high NG were aneuploid or diploid with high S‐phase fraction (SPF), while 20 (77%) of histologic low NG were diploid with low SPF. This study confirms that nuclear grading of FNA of breast carcinoma using a simplified NG system has a high concordance with histology, has high intraobserver and interobserver reproducibility, and that this grading system correlates well with FCM analysis when tumors are simply divided based on NG as high or low grade. Diagn Cytopathol 1994; 11:124–130. © 1994 W

ISSN:8755-1039    

DOI:10.1002/dc.2840110205

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1994

数据来源: WILEY

摘要:

AbstractSixty‐six unselected breast cancers were analyzed in cytologic smears and histologic sections for the expression of Ki‐67, proliferating cell nuclear antigen (PCNA). estrogen receptor protein (ERP), and p53 protein using a standard immunochemical method. The results, expressed as both positive cases and labelling index (LI), were compared with clinical and pathobiological variables. Ki‐67 and PCNA immunostaining was seen in all cases, whereas ERP was detectable in 46/63 cases and p53 protein in 20/66 cases. The expression of these markers was generally lower in cytology than in histology, though the differences were not statistically significant. PCNA‐LI and Ki‐67‐LI were closely correlated (P<0.001), the mean PCNA:Ki‐67 ratio being 0.92 ± 0.57. Occasional discrepancies, however, were found. PCNA and Ki‐67 expression was associated with an increase in histologic grade and a decrease in ERP content of tumors, whereas p53 was statistically associated with no clinical or pathobiological variables. The data suggest that proliferative activity and oncogene overexpression may be reliably evaluated in breast cancer by FNA cytology, though PCNA is not a suitable indicator for cell proliferation. The results do not resolve the issue as to whether immunostaining for p53 protein constitutes a dedifferentiation product of the tumor, or is a fundamental aspect of the malignant progression. Survival studies in a larger series of tumors are thus needed to elucidate this point. Diagn Cytopathol 1994; 11:131–140. © 1

ISSN:8755-1039    

DOI:10.1002/dc.2840110206

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1994

数据来源: WILEY

摘要:

AbstractThe cytologic findings from 50 cases of asymptomatic Hashimoto's thyroiditis diagnosed by fine‐needle aspiration are reviewed. All patients were referred for fine‐needle aspiration by the same physician: 48 because of thyroid enlargement and the clinical suspicion of Hashimoto's thyroiditis, two because of a small thyroid nodule. Positive antimicrosomal (antithyroid peroxidase) and/or antithyroglobulin antibody tilers (performed by the same laboratory using the same technique) were present in only 25 of 48 patients evaluated. Cytologic findings included a predominantly lymphocytic process in 92% of the cases and a predominantly oxyphilic (Askanazy/Hürthle) cell process in 8% of the cases. It is likely that all patients were in an early stage of the autoimmune process. Our data indicate that the incidence of Hashimoto's thyroiditis is considerably higher than when recognized only by serologic tests for antibodies. Diagn Cytopathol 1994; 11:141–145. © 1994 Wiley‐

ISSN:8755-1039    

DOI:10.1002/dc.2840110207

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1994

数据来源: WILEY

摘要:

AbstractTubular carcinoma represents a rare, well‐differentiated type of breast malignancy, characterized by relatively unaggressive clinical behavior. Cytologic diagnosis of this subgroup of tumors is considered difficult as cell population usually presents in tightly cohesive clusters and shows minimal atypia. Cytomorphologic features of 15 examples of histologically proven pure tubular carcinomas of the breast are herein reported. If a constellation of morphologic criteria is critically evaluated, tubular carcinoma may be diagnosed on cytologic specimens.Aspiration biopsy cytology represents an optimal diagnostic tool in the preoperative diagnosis of breast masses. Its application to the detection of this special type of breast carcinoma permits better planning of therapeutic procedures. Diagn Cytopathol 1994; 11:146–150. © 1994 Wiley‐Lis

ISSN:8755-1039    

DOI:10.1002/dc.2840110208

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1994

数据来源: WILEY

摘要:

AbstractThe correlation between DNA ploidy and S‐phase fraction (SPF) by flow cytometry (FCM) and the detection of numerical chromosomal abnormalities (NCA) by interphase cytogenetics (IC) involving chromosomes 8 and 12 was studied in 20 human breast lesions (17 breast carcinomas, 2 fibroadenomas, and 1 sclerosing adenosis). Initial diagnosis was performed on fine‐needle aspiration biopsy (FNAB) material with subsequent histologic confirmation. FCM was performed on formalin‐fixed paraffin‐embedded tissue while IC by fluorescence in situ hybridization (FISH) was done on alcohol fixed FNAB materials. Sixteen (80%) cases showed concordance between FCM and IC with respect to the presence or absence of aneuploidy. The remainder of the cases (20%), which were all malignant neoplasms, showed discrepancies between the two methods, all four were DNA‐diploid with low SPF by FCM but showed various NCA by IC. Nuclear grades (NG) of all the malignant samples were also evaluated and correlated with both FCM and IC studies. Although a good correlation was observed between NG and FCM, a better correlation was seen between NG and IC. This study shows that although IC by FISH correlated well with FCM analysis, it can detect NCA in DNA‐diploid, low SPF tumors. It also correlates well with the NG of the tumor. The increased sensitivity provided by IC in detecting aneuploidy may be of great prognostic significance in low stage, DNA‐diploid, low SPF breast carcinomas. Diagn Cytopathol 1994; 11:151–158. © 1994

ISSN:8755-1039    

DOI:10.1002/dc.2840110209

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1994

数据来源: WILEY

摘要:

AbstractA case study of desmoplastic small round cell tumor with divergent differentiation in a 13‐yr‐old boy is presented. Clinical, cytologic, histologic, immunohistochemical, and ultrastructural findings are reported. There was a close correlation between the various histologic and cytologic patterns. Immunohistochemical studies revealed strong positivity of many of the tumor cells for cytokeratin, vimentin, and desmin. Ultrastructurally, some of the tumor cells contained aggregates of cytoplasmic intermediate filaments. Fine‐needle aspiration biopsy may suggest the diagnosis of desmoplastic small round cell tumor. Confirmation of the diagnosis may be provided by immuhistochemistry and electronmicroscopy. Diagn Cytopathol 1994; 11:159–164. © 1994 Wiley

ISSN:8755-1039    

DOI:10.1002/dc.2840110210

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1994

数据来源: WILEY

摘要:

AbstractA series of 36 cases of breast carcinomas diagnosed by fine‐needle aspiration were investigated for the presence of estrogen receptors (ER) and the estrogen‐induced pS2 protein. Immunocytochemically ER could be demonstrated in 28 aspirates, whereas eight were negative. These eight were also negative for the pS2 protein. In addition three aspirates with a low score for ER and five with a high score for ER were found negative for pS2. Thus, of the 36 cases, 20 were found to express the pS2 protein. The presence of the pS2 protein in the tumor cells is believed to be a marker of a functional estrogen regulatory system and its demonstration may therefore predict clinical responsiveness to hormonal therapy. Diagn Cytopathol 1994;11:165–167. © 1994 Wiley‐L

ISSN:8755-1039    

DOI:10.1002/dc.2840110211

出版商:Wiley Subscription Services, Inc., A Wiley Company    

年代:1994

数据来源: WILEY