摘要:

A flow system has been developed for a fast and convenient assay of LD-1 and total LD in serum. The LD-1 is separated immunochemically and subsequently determined amperometrically by a thin layer transducer in a flow system. Potassium ferricyanide was used as a mediator at a potential of 0.07 V vs Ag/AgCl to eliminate any protein fouling of the electrode. A kinetic measurement helps to diminish several interferences in the electrode response. Thus, it is possible to determine the LD-1 or LD-1/total LD conveniently and accurately even at low activities.

ISSN:0003-2719    

DOI:10.1080/00032718508066137

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

A new assay ofL-aspartate:2-oxoglutarate aminotransferase (EC 2, 6, 1, 1) (glutamic-oxaloacetic transaminase: GOT) is reported. SubstrateL-aspartate was replaced withL-cysteine sulfinic acid (CSA) which yields sulfite and pyruvate. The enzyme activity was determined by measuring the sulfite with the aid of a fluorescent reagent, N-(9-acridinyl)maleimide (NAM). The method presents a extremely sensitive GOT assay in the range of 4 to 140 U/l using less than 10 μl of human serum.

ISSN:0003-2719    

DOI:10.1080/00032718508066138

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

The feasibility, efficiency and advantages of the HPLC method for the analysis of enzyme mixtures, associated with cell walls, is demonstrated with two different types of macroporous columns.

ISSN:0003-2719    

DOI:10.1080/00032718508066139

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

The application of infrared absorption spectrophotometry the simulataneous determination of the predominant serum lipids is discussed. Quantification of the lipid extract from a 200 μl serum aliquot is based on the difference in the ester carbonyl absorption frequencies of triglycerides (νmax= 1742 cm−1), phospholipids (νmax= 1737 cm−1) cholesterol (νmax= 1723cm−1). Using a least-squares curve fitting technique in P-matrix notation, the optimum models for the Beer-Lambert Plots are C = P1A + POfor triglycerides and cholesterol and C = P2A2+ P1A + POfor phospholipids. Serum samples whose concentrations are outside of calibration limits (triglycerdes 30–209 mg/dl, phospholipids 72–385 mg/dl, and cholesterol 60–300 mg/dl) can be brought into range by appropriate dilution. Coefficients of variation (within-run) were less than 6% for all three components. Results of the infrared method are compared with standard clinical procedures.

ISSN:0003-2719    

DOI:10.1080/00032718508066140

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

This is a scanned image of the original Editorial Board page(s) for this issue.

ISSN:0003-2719    

DOI:10.1080/00032718508066126

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor