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11. |
Production of Specific Antibodies Against Diethylistilbestrol |
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Analytical Letters,
Volume 5,
Issue 7,
1972,
Page 479-486
GuyE. Abraham,
EdwardM. Reifman,
JohnE. Buster,
JosephDi Stephano,
JohnR. Marshall,
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摘要:
Specific antibodies against diethylstibestrol (DES) were obtained from one ewe immunized against DES-Succinate-human serum albumin conjugate. Using tritiated DES as a tracer, a radioimmunoassay for DES was developed. The senstivity of the standard curve was between 0.25 and 0.5 ng, using a mass of tracer of about 0.5 ng. The antiserum did not cross react with any naturally occurring steroid tested. After oral administration of 10 mgm of DES to a male subject, plasma DES measured after ether extraction reached peak levels of 18.4 and 18.6 ng/ml of plasma 1 and 2 hours later. Plasma DES was still detectable 10 hours after administration. Proof of the validity of this assay for the measurement of DES in cattle would require testing the interference of many plant nonsteroidal estrogen chemically related to DES.
ISSN:0003-2719
DOI:10.1080/00032717208064330
出版商:Taylor & Francis Group
年代:1972
数据来源: Taylor
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12. |
Radioimmunoassay of Plasma 16a-Hydroxydehydroepiandrosterone |
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Analytical Letters,
Volume 5,
Issue 7,
1972,
Page 487-495
JohnE. Buster,
GuyE. Abraham,
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摘要:
This report describes a radioimmunoassay method for the measurement of unconjusted 16a-hydroxydehydroepiandrosterone (16a-DHEA) in plasma. The assay uses a specific anti16a-DHEA antiserum which cross reacted completely with dehydroepiandrosterone (DHEA) and to a lesser extent with androstenetriol and androstenediol. A chromatographic system, using celite microcolumns, separated 16a-DHEA from the cross reacting steroids prior to its measurement. Using this simple technique, we have measured 16a-DHEA accurately in small aliquots of plasma from newborns and adults of both sexes. The highest levels of 16a-DHEA were found in newborn infants with a mean value of 16.1 ng/ml. Pregnant women at term had a mean level of 3.2 ng/ml. The levels of 16a-DHEA were low in adults of both sexes with mean values of 0.66 and 0.87 ng/ml for male and female subjects respectively
ISSN:0003-2719
DOI:10.1080/00032717208064331
出版商:Taylor & Francis Group
年代:1972
数据来源: Taylor
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13. |
Novel Copper-Tartrate Coordination Compounds |
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Analytical Letters,
Volume 5,
Issue 7,
1972,
Page 497-508
NeilD. Jespersen,
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摘要:
Using the thermometric enthalpy titration methodology, the hexa-ligand species CuT6[where T represents the tartrate ion and charges are omitted] has been shown to exist even in dilute tartrate solutions. Kinetic considerations suggest a radical difference between CuT3CuT2and the more highly coordinated species CuT3, CuT4, CuT5and CuT6. It is evident that the hydroxyl groups on the tartrate ion are singularly important contributers to this effect
ISSN:0003-2719
DOI:10.1080/00032717208064332
出版商:Taylor & Francis Group
年代:1972
数据来源: Taylor
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14. |
Editorial board page for “Analytical Letters”, Volume 5, Number 7 |
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Analytical Letters,
Volume 5,
Issue 7,
1972,
Page -
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PDF (81KB)
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摘要:
This is a scanned image of the original Editorial Board page(s) for this issue.
ISSN:0003-2719
DOI:10.1080/00032717208064319
出版商:Taylor & Francis Group
年代:1972
数据来源: Taylor
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