摘要:

A high-performance liquid chromatographic method is described for the simultaneous measurement of carbamazepine, phenobarbital and their major metabolites in small samples of serum, brain tissue and urine. This involves solvent extraction, reversed-phase chromatography and ultraviolet detection at 195 nm. Glucuronides in urine are hydrolyzed by enzymatic cleavage with β-glucuranidase. Quantitation is based on the peak-height ratio of the analyte to its internal standard (10, 11-dihydrocarbamaze-pine or p-methylphenobarbital). The results obtained show that the method is precise and reproducible.

ISSN:0003-2719    

DOI:10.1080/00032718708064608

出版商:Taylor & Francis Group    

年代:1987

数据来源: Taylor

摘要:

Square-wave adsorptive stripping voltammetry has been applied to the determination of a new cytostatic anthracyclinic compound, marcellomycine. 100-fold and 1000-fold increases of sensitivity have been found with regard to the differential pulse stripping voltammetry and differential pulse polarography respectively. The detection limit is fixed at 5 × 10−9M.

ISSN:0003-2719    

DOI:10.1080/00032718708064609

出版商:Taylor & Francis Group    

年代:1987

数据来源: Taylor

摘要:

Phosphoramide mustard (PM), a key active metabolite of the widely used anticancer drug cyclophosphamide (CP), can exist in several closely-related ionized, cyclized and substituted forms. We have developed a high pressure liquid chromatographic (HPLC) method for analysing serum concentrations of PM in order to relate these serum concentrations to toxicity and efficacy of treatment of CP.31p NMR spectroscopy is used to verify the HPLC peak homology of the proposed PM peak.

ISSN:0003-2719    

DOI:10.1080/00032718708064610

出版商:Taylor & Francis Group    

年代:1987

数据来源: Taylor

摘要:

A continuous flow bioluminescent method for L-alanine analysis in serum and urine has been developed. Serum can be analyzed directly after simple filtration. Response is linear from 50 to 1500 pmoles in biological matrix. Alanine dehydrogenase is immobilized onto a nylon coil separated from the reactor coil containing bioluminescent enzymes. The stability of nylon immobilized enzymes is high (over three months) and more than 900 samples can be analyzed with few mg of enzymes. The results obtained with the bioluminescent sensor agree well with those obtained by ion exchange chromatography (amino acid analyzer).

ISSN:0003-2719    

DOI:10.1080/00032718708064611

出版商:Taylor & Francis Group    

年代:1987

数据来源: Taylor

摘要:

This is a scanned image of the original Editorial Board page(s) for this issue.

ISSN:0003-2719    

DOI:10.1080/00032718708064597

出版商:Taylor & Francis Group    

年代:1987

数据来源: Taylor