摘要:

Two rapid assay procedures based on high performance liquid chromatography (HPLC) and derivative ultraviolet (UV) spectrophotometry have been developed for the simultaneous determination of pindolol and clopamide in two component tablets. An HPLC assay with RP-18 column, of 250 × 4.6 mm ID as dimension and 10 μm silica particles, was used. The mobile phase consisted of water-methanol-acetonitrile (50–45–5) with pH of 3 and a flow rate of 1.5 ml min−1. Detection was at 264 nm with a band width of 10 nm. The derivative spectrophotometric procedure depends on the measurements of2D amplitude in methanol at 264 nm for pindolol and ▴1D amplitude of the delta absorption spectra (methanolic HCl -methanolic NaOH) at 249 nm for clopamide. For HPLC procedure the calibration graphs were linear in the ranges of 10–20 and 5–10 μg ml−1for pindolol and clopamide, respectively. For the derivative method, they were linear in the ranges of 10–26 and 10–20 μg ml−1for pindolol and clopamide, respectively. The calibration graphs showed a good linearity and obedience to Beer's law. Commercial tablets and laboratory-made mixtures containing both pindolol and clopamide in different proportions were assayed by the developed procedures. The results were of comparable accuracy as indicated by a statistical analysis of the data, using both t- and F-tests.

ISSN:0003-2719    

DOI:10.1080/00032719508001432

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

摘要:

A rapid, simple and sensitive fluorimetric method has been developed for the determination of atmospheric sulphur dioxide with dichlorofluorescein as fluorogenic reagent (λex= 505 nm, λem= 520 nm) at pH 4.0–6.0. A linear calibration curve was obtained in the range 0.01–0.40 μg SO2/25 ml. The detection limit is 0.01 μg SO2/25 ml. Nitrogen dioxide does not interfere with the method. The method was successfully applied to the determination of atmospheric sulphur dioxide.

ISSN:0003-2719    

DOI:10.1080/00032719508001433

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

摘要:

Micellar electrokinetic capillary chromatography (MECC) with on-column UV detection at 214 nm, was used to detect and determine platinum(II) in the presence of Rh(III), Ru(III), Os(III) and Ir(III) in acidic medium (HCl+KCl, pH=3.0). Under an applied voltage of 15 KV, platinum(II) tetrachloride (PtCl2−4) was separated and detected in less than 4 min. A cathodic injection and anodic detection scheme was used. The electroosmotic flow was reversed by a cationic surfactant (CTMAB) and the analysis time was shortened. The linear calibration range is 0.2–40 μg/ml. The detection limit is 0.2 μg/ml. The method is most useful for samples digested in HCl/HNO3. The method is simple, rapid and highly selective.

ISSN:0003-2719    

DOI:10.1080/00032719508001434

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

ISSN:0003-2719    

DOI:10.1080/00032719508001435

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

ISSN:0003-2719    

DOI:10.1080/00032719508001421

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor