摘要:

A reverse-phase HPLC system using a microparticulate octade-cylsilane column and an acetonitrile-phosphate buffer mobile phase is employed to determine lidocaine in an admixture of lidocaine injection with 5 per cent dextrose injection. The method is accurate over the range of 5 to 12.5 μg on column, as employed in our laboratories, which corresponds to 2 to 5 mg/ml of lidocaine hydrochloride in the admixture solution. It is precise (2.00 to 2.88% coefficient of variation) and has been used in stability studies of lidocaine. The method is also used to quantitate a degradation product of lidocaine, 2,6-dimethylaniline, over the range of 5 to 25 ng on column (corresponding to 2 to 10 μg/ml in the admixture solution) with a precision of 1.82 to 4.50% coefficient of variation. Samples are prepared by dilution in methanol containing the internal standard, procaine hydrochloride, and each chromatogram requires about 4 minutes.

ISSN:0003-2719    

DOI:10.1080/00032718108055470

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

A quantitative analysis of salicylamide in microplasma volumes by high-performance 1iquid chromatography using fluorescence detection is reported. The procedure is extremely simple and very rapid, involving the direct introduction of the plasma sample on the HPLC column. The assay procedure is linear over the concentration range studied, 0–100 ng/ml with correlation coefficient for the linear regression, r = 0.998. This assay procedure enables the detection of salicylamide as low as 5.0 ng/ml in plasma, using sample volume of 100 μl.

ISSN:0003-2719    

DOI:10.1080/00032718108055471

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

A method is described for the analysis of the non-steroidal anti-estrogenic antineoplastic agent, tamoxifen and its 4-hydroxy and N-desmethyl metabolites in liver, uterine, fat and human breast tumor tissue, and whole blood, plasma and cerebral spinal fluid. The report focuses on separation of analytes from the biological matrix. After extraction, analytes are converted to fluorescent phenylphenanthrenes, which are separated by reverse phase paired ion chromatography with spectrofluorometric monitoring of column eluent. By control of column temperature, chromatographic analysis time could be significantly reduced and sensitivity increased.

ISSN:0003-2719    

DOI:10.1080/00032718108055472

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

A rapid and selective method using high performance liquid chromatography with electrochemical detection is described for the determination of apomorphine in tablets. Tablet mixes were dissolved in a standard volume of mobile phase containing the internal standard, N-n-propylnorapomorphine. Separation was achieved on a μ-phenyl column using methanol-acetonitrile-0.05M KH2PO4(5:15:80) as mobile phase. The eluted compounds were detected with a sandwich-type electrochemical detector employing a glassy carbon working electrode and operated at 0.5V. Satisfactory accuracy and precision were obtained during analyses of tablets containing apomorphine.

ISSN:0003-2719    

DOI:10.1080/00032718108055473

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

The novel method now described, forS-carboxymethylcysteine in plasma at levels that may be lower than 20 μg/ml in therapeutic investigations, entails conversion into the corresponding phenylthiohydantoin (PTH) derivative, so as to confer ultraviolet detectability in a final separation step entailing reverse-phase HPLC. The conditions adopted take account of possible losses of the compound, associated with capricious water-solubility, of stability problems unexpectedly encountered with its PTH derivative, and of the risk of interferences from plasma constituents.

ISSN:0003-2719    

DOI:10.1080/00032718108055474

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

The previous chronoamperometric method described for the determination of lecithin in ethanolic-aqueous medium based on the inhibition of the oxygen reduction process at the dropping mercury electrode is applied to the determination of lecithin and sphingomyelin in methanolic-aqueous solution. The method permits the analysis of these phospholipids in isolation, or the sum of them when they are found in mixture, in the 4–40 μg/ml range (20–200 pg of phospholipids) with a standard deviation of 1%. The effect of the surface-active substances present in amniotic fluid on the inhibition of the oxygen reduction process is similar to the lecithin and sphingomyelin commercial samples giving directly chronoamperometric curves suitable for a quantitative evaluation of surfactants in amniotic fluid.

ISSN:0003-2719    

DOI:10.1080/00032718108055475

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

Cross-linked agarose activated with carbonyl diimidazole and subsequently coupled to aminophenyl boronic acid provides a highly efficient matrix for separation and quantitation of glycosylated hemoglobins The method described is highly reproducible. rapid and flexible in that it can be adapted to column or batch formats The method can tolerate moderate fluctuations in temperature and pH of buffeused without significantly altering results Hemoglobin fractions separated by this affinity support were Subjected to isoelectric focusing which revealed a heterogeneous population of glucose modified proteins.

ISSN:0003-2719    

DOI:10.1080/00032718108055476

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

ISSN:0003-2719    

DOI:10.1080/00032718108055469

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor