摘要:

A glc method utilizing a flame ionization detector was developed for determing secobarbital (I) in human plasma and urine. Extraction of plasma and urine to which the internal standard, allylcyclopental barbituric acid (II) had been added was accomplished using benzene. An on-column methylation technique was used to facilitate glc analysis. Accuracy and precision of the method was demonstrated over the concentration range anticipated in acute dosing human studies. The method was evaluated in one subject who received two different oral doses of I four weeks apart and found to be applicable to plasma and urine analysis. Saliva was also collected from this subject and analyzed by the method. Although I could be detected at one time interval, quantification could not be accomplished due to lack of sufficient sensitivity.

ISSN:0003-2719    

DOI:10.1080/00032718208064387

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

A reverse-phase HPLC system using a microparticulate hexyl bonded-phase column and a methanol-water mobile phase was employed to quantitate diazepam in its injectable formulation. The method was accurate over the range of 15 to 150 ng on column, as employed in our laboratory, corresponding to 0.6 - 6 mg/ml of diazepam in the injection solution. It was precise (3.97 to 4.63% coefficient of variation) and has been used in stability studies of diazepam. The method was also used to quantitate a degradation product of diazepam, 2-methylamino-5-chlorobenzophenone, over the range of 0.5 to 4.5 ng on column (corresponding to 20-180 ug/ml in the injection solution) with a precision of 2.18 to 7.07% coefficient of variation. Samples were prepared by dilution with methanol containing biphenyl as internal standard. Each chromatogram required about 6 minutes.

ISSN:0003-2719    

DOI:10.1080/00032718208064388

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

A simple and specific technique is used in analyzing pharmaceutical preparations (capsules, elixirs, syrups, and tablets) for theophylline and related compounds. The procedure consists of dissolving (diluting) appropriate amounts of the preparations and standards in (with) a 1 M KI-water solution, spotting 5 μL of each resultant solution onto filter paper discs, determining the phosphorescence intensities at room temperature and comparing sample signal levels to those of standards. The quantities of active ingredients and standards used in the determinations ranged from 25 ng to 1 μg. The results indicate that room temperature phosphorescence is useful for analyzing pharmaceutical preparations where active ingredients are usually contained in a wide variety of matrices.

ISSN:0003-2719    

DOI:10.1080/00032718208064389

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

In this present work, we have applied the conductimetric method to measure cholinesterase inhibition by pesticides.

ISSN:0003-2719    

DOI:10.1080/00032718208064390

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

An enzyme electrode containing immobilized GOD was utilized in a continuous flow system for the automatical determination of glucose. The advantages of kinetic measurement of H2O2are used. Characteristic parameters of device and results in routine are shown. Particular advantages are: low requirement on material (20 μl blood, < l μg GOD/measurement) and time (60 samples/h at 15 sec response time) as well as sufficient precision and accuracy (S % - serial: < 2 %).

ISSN:0003-2719    

DOI:10.1080/00032718208064391

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

In this study, the enzymatic oxidation of acetaldehyde, one of the substrates of xanthine oxidase, has been studeid by a microcalorimetric method. A flow microcalorimeter is used to measure the heat changes during the during the reaction. The catalytic determination of xanthine oxidase between the concentrations 0.2×10−1and 2.4×10−1mg/ml (0.8×10−3−9.6×10−3U/ml) has been achived keeping acetaldehyde concentration constant at 4.0×10−3M and the catalymetric determination of acetaldehyde between 0.8×10−3and 2.8×10−3M is accomplished at a fixed concentration of enzyme.

ISSN:0003-2719    

DOI:10.1080/00032718208064392

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

A radioiodination technique is described which consists of heating Testosterone (T) with Na125I and hydrogen peroxide in acetic acid in sealed vial. The binding capacity of labelled substances was measured after thin layer chromatography of the reaction mixture. One compound was selected for radioimmunoassay on the basis of its binding capacity for anti-T serum. Standard curves utilizing tritiated and radioiodinated T, under identical conditions, were superimposible. Serum hormone concentrations determined by the two tracers were comparable. Accuracy, precision and sensitivity were satisfactory. Mass spectroscopy and nuclear magnetic resonance of the unlabelled iodinated T indicated that iodine is in C-2.

ISSN:0003-2719    

DOI:10.1080/00032718208064393

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

This is a scanned image of the original Editorial Board page(s) for this issue.

ISSN:0003-2719    

DOI:10.1080/00032718208064386

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor