摘要:

A theoretical basis for enzyme amplified receptor assay (ERA) is presented and criteria are established for the general case of any receptor system. Analytical criteria necessary to perform this assay are identified based upon the relevant affinity constants, the concentrations of the enzyme-labelled ligand and receptor, and the analyte.

ISSN:0003-2719    

DOI:10.1080/00032718808071921

出版商:Taylor & Francis Group    

年代:1988

数据来源: Taylor

摘要:

A new fiber-optic probe for the determination of glucose is developed. Glucose oxidase is immobilized on a preactivated Immunodyne membrane by direct application of the enzymatic solution to one side of the membrane. The membrane is then placed around the common end of a bifurcated glass fiber optic bundle, and immersed in the sample cell which contains the glucose sample, peroxidase and a colorless dye. Glucose is quantified by the color change when the reaction takes place.

ISSN:0003-2719    

DOI:10.1080/00032718808071922

出版商:Taylor & Francis Group    

年代:1988

数据来源: Taylor

摘要:

An electrochemically prepared micro-enzyme electrode whose diameter is 50 jim is combined with an Pt auxiliary electrode and a reference electrode to assemble a three electrode device for the rapid determination of glucose. Since the device is very small, glucose sample whose volume is only 2 μ1 can be successfully determined. Pulse voltammetry is shown to be an effective approach for making the sensing device work without any attachments such as magnetic stirrer and pump. The transient sensor output, oxidizing current for the hydrogen peroxide generated by the immobilized glucose oxidase, shows a good linearity in the glucose concentration range from 1 mM to 20 mM.

ISSN:0003-2719    

DOI:10.1080/00032718808071923

出版商:Taylor & Francis Group    

年代:1988

数据来源: Taylor

摘要:

A new method is developed to determine picomole levels of phosphatidylcholine hydroperoxides (PC-HPO) in human plasma. The PC-HPO was extracted from 500 μ1 of human plasma with a mixture of chloroform and methanol and separated on a HPLC with a silica gel column using the chloroform - methanol - water system. Detection was carried out by fluorometry after post column derivatization with diphenyl-1-pyrenylphosphine CDPPP). The determination of PC-HPO was performed by the method of standard addition. The sampling error was corrected by using the UV peak of inherent unoxidized PC as an internal standard. By this method, the PC-HPO levels in healthy adult human plasma were 20–40 pmol/ml which compares favorably to the normal expected values.

ISSN:0003-2719    

DOI:10.1080/00032718808071924

出版商:Taylor & Francis Group    

年代:1988

数据来源: Taylor

摘要:

A procedure is described for the DP-polarographic determination of trichloroacetic acid (TCA) in water solution and in urine. In the latter case a very simple pretreatment of samples is suggested and a detection limit comparable with that of spectrophotometric and chromatographic methods is found. The application of this procedure for the determination of TCA as metabolite in workers exposed to trichloroethylene vapours is proposed.

ISSN:0003-2719    

DOI:10.1080/00032718808071925

出版商:Taylor & Francis Group    

年代:1988

数据来源: Taylor

摘要:

A differential kinetic fluorimetric method is proposed for the simultaneous determination of thiamine (vitamin B) and its pyrophosphate ester (cocarboxylase). It is based on the oxidation of these analytes by mercury(II) in a basic medium to yield fluorescent thiochrome. The calibration graph of each analyte is linear in the range 4 × 10−8−3 × 10−5M. The logarithmic extrapolation method is used to resolve thiamine/cocarboxylase mixtures in ratios between 4:1 and 1:15. Results of precision and selectivity studies are reported. Thiamine and cocarboxylase are satisfactorily determined in synthetic multivitamin preparations by the proposed method. A method for the individual determination of cocarboxylase is also described and applied to the analysis of this analyte in commercial pharmaceutical preparations.

ISSN:0003-2719    

DOI:10.1080/00032718808071926

出版商:Taylor & Francis Group    

年代:1988

数据来源: Taylor

摘要:

A fully enzymatic method for the colorimetric determination of serum and urine creatinine is described which does not require sample blank measurements. It is based on the formation of hydrogen peroxide from creatinine in a reaction sequence catalyzed by creatinine iminohydrolase, ATP-dependent 1-methylhydantoinase, N-carbamoylsarcosine amidohydrolase and sarcosine oxidase. The hydrogen peroxide is quantitated with high sensitivity at 546 nm by a chromogenic system consisting of peroxidase, 2′-sulpho-2-methyl-benzthiazolinone hydrazone and 2,4,6-tribromo-3-hydroxy-benzoic acid. Only 20 μL of sample are needed for the assay, the total reaction being complete within 10 min at 25°. Within-run precision gave a CV of 3.1 and 1.6 % at serum creatinine concentrations of 79 and 160 μmol/L, respectively, and the standard curve is linear up to at least 1760 μmol/L. The assay yields results which agree well with those found by both an enzymatic UV-method and an alternate enzymatic colorimetric procedure necesitating sample blank measurements to correct for endogenous creatine.

ISSN:0003-2719    

DOI:10.1080/00032718808071927

出版商:Taylor & Francis Group    

年代:1988

数据来源: Taylor

摘要:

A novel enzyme immunoassay (immune complex transfer enzyme immunoassay) for anti-insulin IgG in guinea pig serum is described. Anti-insulin IgG in guinea pig serum was reacted with dinitrophenyl bovine serum albumin-insulin-horseradish peroxidase conjugate, and the complex formed between guinea pig anti-insulin IgG and the conjugate was trapped onto affinity-purified rabbit (anti-dinitrophenyl bovine serum albumin) IgG-coated polystyrene balls in the presence of bovine serum albumin. After eliminating nonspecific guinea pig IgG in test serum, the complex was eluted from the polystyrene balls with dinitrophenyl-L-lysine and transfered to clean polystyrene balls coated with affinity-purified rabbit (anti-guinea pig IgG)IgG. Peroxidase activity bound to the (anti-guinea pig IgG) IgG-coated polystyrene balls was measured by fluorimetry. The detection limit of anti-insulin IgG in guinea pig serum was lowered 1,000 to 10,000-fold as compared with that by the conventional enzyme immunoassays.

ISSN:0003-2719    

DOI:10.1080/00032718808071928

出版商:Taylor & Francis Group    

年代:1988

数据来源: Taylor

摘要:

To determine phosphatidylcholine hydroperoxide (PCOOH) in human plasma lipids, a chemiluminescence -high performance liquid chromatography (CL-HPLC) system was newly developed and applied. This system * To whom correspondence should be addressed. consisted of separation of phosphatidylcholine derivatives with normal phase HPLC and detection of hydroperoxide-specific chemiluminescence of PCOOH. By use of this system, the PCOOH in normal human plasma was quantitatively assayed to be 28-431 pmol hydroperoxide-02 per ml of plasma. The high sensitivities, reproducibilities and specificities of the CL-HPLC assay were shown for determining plasma PCOOH.

ISSN:0003-2719    

DOI:10.1080/00032718808071929

出版商:Taylor & Francis Group    

年代:1988

数据来源: Taylor

摘要:

A derivative spectroscopy technique for the simultaneous determination of plasma amitriptyline and perphenazine is proposed. The described method is fast and requires only one extraction for both drugs.

ISSN:0003-2719    

DOI:10.1080/00032718808071930

出版商:Taylor & Francis Group    

年代:1988

数据来源: Taylor