摘要:

Based on the detection of agglutination of antibody- or antigen-bearing polymer by an immunoreaction using a piezoelectric quartz crystal, a piezoelectric immunoassay technique was developed for the determination of human serum albumin (HSA). The effect of experimental conditions including the polymer concentration, the antibody dilution ratio and the reaction temperature on the frequency response were investigated. The frequency responses were linearly correlated to the concentrations of HSA in the range of 112 – 878 μg ml−1. Other antigens present in serum did not interfere significantly with the determination of HSA. Analytical results of five clinical specimens obtained using the developed technique were in satisfactory agreement with those given by the rate diffusion turbidimetry.

ISSN:0003-2719    

DOI:10.1080/00032719708001697

出版商:Taylor & Francis Group    

年代:1997

数据来源: Taylor

摘要:

Disposable Clark-type oxygen electrodes were fabricated using filter paper as the substrate of the electrodes, which also served to absorb the electrolyte required. The oxygen electrode has a two-electrode configuration and both the anode and cathode were deposited directly on filter paper by sputtering a layer of silver. Silicone rubber was used as the gas-permeable membrane. The whole fabricating procedure dispensed with the expensive photolithographic process used in silicon- and glass based oxygen electrodes, decreasing both the cost and time required for fabrication. The electrodes showed good characteristics for voltammetrically detecting dissolved oxygen and their dynamic range covered the entire possible range of dissolved oxygen concentrations. The electrodes had a 90% response time of 12 seconds and a life time of about 2 hours.

ISSN:0003-2719    

DOI:10.1080/00032719708001698

出版商:Taylor & Francis Group    

年代:1997

数据来源: Taylor

摘要:

Molecular imprinting of cyclosporin A was undertaken and the resultant polymer preparation was applied to the development of a radioligand binding assay for determination of the total concentration of cyclosporin A and its metabolites. The assay method comprised extraction of haemolyzed whole blood with diisopropyl ether followed by competitive radio-‘immuno’-assay of the organic phase employing imprinted polymer particles and [3H]cyclosporin. The assay measured the parent drug and the metabolites AM1, AM9 and AM4N with equal response. Concomitant analysis of patient samples using a specific EMIT assay in parallel with this novel imprinted polymer-based assay permitted simultaneous determination of the concentration of cyclosporin A and that of its metabolites.

ISSN:0003-2719    

DOI:10.1080/00032719708001699

出版商:Taylor & Francis Group    

年代:1997

数据来源: Taylor

摘要:

Previously, we reported the effect of N-acetylcysteine administration on the concentration of total plasma homocysteine in patients with chronic renal failure. Total plasma NAC was determined before and after p.o. administration of NAC as a measure of patient compliance. However, we did not fully describe the method by which the total plasma N-acetylcysteine was measured, and we did not report the levels of total plasma N-acetylcysteine before or after administration of N-acetylcysteine.

ISSN:0003-2719    

DOI:10.1080/00032719708001700

出版商:Taylor & Francis Group    

年代:1997

数据来源: Taylor

摘要:

The UV spectrophotometric investigations obtained for the reaction between L-DOPA and copper(II)ion contribute to the clarification of equilibrium conditions of the complex formation both in the excess of metal ion and ligand. For the complex obtained in the excess of copper(II)ion optimum conditions were established, as well as the stoichiometric composition (1:1) and conditional stability constant logK' = 5.70±0.05. The proposed method, using copper(II)ion, as the analytical reagent in excess, for the UV spectrophotometric determination is suitable for accurate, sensitive and selective analysis of L-DOPA both in pure and dosage forms containing benzerazide.

ISSN:0003-2719    

DOI:10.1080/00032719708001701

出版商:Taylor & Francis Group    

年代:1997

数据来源: Taylor

摘要:

A simple sensitive spectrophotometric method is described for determination of six drugs with different n-donating groups. These drugs are azithromycin (I), ketoconazole (II), clotrimazole (III), clozapine (IV), oxamniquine (V), and pimozide (VI). The method depends on the interaction of these compounds with 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) as π acceptor to form highly colored reaction product. The analytical wavelength chosen is 588 nm which gives maximum sensitivity. Optimum conditions for the reaction were determined and the mean percentage recoveries were 99.79 ± 0.65, 100.44 ± 1.03, 100.12 ± 0.66, 99.60 ± 0.8,99.74 ± 0.65 and 100.29 ± 0.79 for I, II, III, IV, V and VI, respectively. Study of the reaction product showed that charge transfer complex (CT) primarily formed dissociates to give radical anion with λmaxat 429 nm. The effect of basic properties of the studied drugs, on the production of radical anion, was illustrated by determining the yield percentage and confirmed through the calculation of the rate constant (K) and the association constant (KCTAD).

ISSN:0003-2719    

DOI:10.1080/00032719708001702

出版商:Taylor & Francis Group    

年代:1997

数据来源: Taylor

摘要:

Triamcinolone acetonide in ointment was determined by first-derivative ultraviolet spectrophotometry and by high performance liquid chromatography. The UV derivative spectrophotometric method eliminated the interference of the ointment excipients when the determination was made at 274 nm, Δλ = 4 nm, scan speed of 300 nm/min, and ordinate settings ±0.050. The correlation coefficient and the relative standard error of the estimate were, respectively, 0.9999 and 0.39%. The coefficient of variation and the recovery average were 0.48 and 99.77%, respectively. The chromatographic methods were standardized using a LiChrospher® 100 RP-18 (5μm) in LiChroCART® (125-4) column, UV detection at 254 nm and mobile phases consisting of: (1) acetonitrile-water (35:65 v/v) and (2) methanol-water-96% acetic acid (55:44:1 v/v), apparent pH 3.0. The peak area response versus concentration was linear at a concentration range from 10.0 to 100.0 μg/mL. The correlation coefficients and the relative standard errors of the estimate were 0.9995, 1.84% and 0.9998 and 1.14% for methods 1 and 2, respectively. The coefficients of variation and the recovery averages were 0.66%, 99.84% and 0.60%, 99.36% for methods 1 and 2, respectively.

ISSN:0003-2719    

DOI:10.1080/00032719708001703

出版商:Taylor & Francis Group    

年代:1997

数据来源: Taylor

摘要:

A method is proposed for the determination of Teicoplanin by differential pulse polarography (DPP). The electroactive product was obtained by nitration with potassium nitrate in sulphuric medium. It showed a reduction peak at -0.1 volts, which may be used for analytical purposes. The optimum conditions for the polarographic signal were determined and a study was made of the different parameters affecting the electrochemical process. A polarographic procedure is proposed for the determination of Teicoplanin in a concentration range between 5.0×10−6M and 1.4×10−4M. The detection limit is about 7.1×10−7M and the relative standard deviation is 3.8%. The method was applied to the determination of Teicoplanin in pharmaceutical formulations.

ISSN:0003-2719    

DOI:10.1080/00032719708001704

出版商:Taylor & Francis Group    

年代:1997

数据来源: Taylor

摘要:

A colorimetric method for the quantitative determination of diclofenac sodium and mefenamic acid in pure form and in pharmaceutical preparations was developed. It was based on the inter-action of the secondary aromatic amine with p-dimethyl-aminocinnamaldhyde in acidified absolute methanol medium to form very stable red [λmaxat 538 nm in case of diclofenac sodium] or blue [λmaxat 665 nm in case of mefenamic acid] products. Beer's law was obeyed over the ranges 10–80 μg ml−1and 1–8 μg ml−1for diclofenac sodium and mefenamic acid, respectively. The reactants were heated on a boiling water bath for 6 and 5 mins for each drug, respectively. Optimization of the different experimental conditions were studied. The mean percentage recoveries were found to be 100.73 ± 0.87% & 100.73 ± 0.44%, respectively. The method was applied successfully for the determination of some pharmaceutical formulations.

ISSN:0003-2719    

DOI:10.1080/00032719708001705

出版商:Taylor & Francis Group    

年代:1997

数据来源: Taylor

摘要:

A highly sensitive and specific fluorimetric method was developed for the determination of nalidixic acid. The proposed method involves the formation of a ternary complex with Tb3+in the presence of hexamine. The different experimental parameters were carefully studied and incorporated into the procedure. Under the described conditions, the proposed method is applicable over the concentration range of 0.1 – 2.4 μg ml−1with a detection limit (S/N = 2) of 2 ng ml−1. The recoveries from spiked urine and plasma were 101.40 and 96.98 percent, respectively.

ISSN:0003-2719    

DOI:10.1080/00032719708001706

出版商:Taylor & Francis Group    

年代:1997

数据来源: Taylor