摘要:

A rapid and sensitive high-performance liquid chromatographic method for the determination of diclofenac sodium in plasma has been developed. The method is specific and free of interference from metabolites and common anti-inflammatory agents. The UV detector (215 nm) response was linear over a range of 5-1000 ng/ml. Day-to-day and within-day calibration curves were reproducible. The method was validated by analysis of spiked human plasma samples, partly in a blind fashion. The accuracy and precision of the method are satisfactory over the range of 5-1000 ng/ml. The method was cross-checked with the GC method. Results show a correlation coefficient of 0.983 and a slope of 1.04. The method is suitable for the routine analysis of large numbers of plasma samples usually obtained in bioavailability and pharmacokinetic studies.

ISSN:0003-2719    

DOI:10.1080/00032718208065144

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

A glc analysis method was developed for the simultaneous determination of diazepam (I), and its major metabolitesN-desmethyldiazepam (II), oxazepam (III), and hydroxydiazepam (IV) in human plasma, urine, and saliva. Medazepam (V) was used as the internal standard to control extraction efficiency and permit precise and accurate determination of I-IV. Extraction and glc analysis of physiological fluid samples in triplicate required only 40 minutes using the developed method. Three human subjects were given either 20 or 5 mg of I via oral administration and serial specimens of blood, urine, and saliva collected. All samples were analyzed using the developed assay method. Results indicate that the method could be applied to pharmacokinetic studies of I where plasma, urine, and/or saliva is to be monitored.

ISSN:0003-2719    

DOI:10.1080/00032718208065145

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

The coccidiostat monensin can be determined by voltammetry at the interface between two immiscible electrolyte solutions (ITIES) in the concentration range between 0.05 mM to 3 mM. The peaks obtained with cyclic voltammetry at the water/nitrobenzene interface correspond to the transfer of sodium ions from the aqueous to the nonaqueous phase facilitated by complexation with monensin functioning as an ionophore and are proportional to its concentrations. The method was applied to the extracts fromStreptomycescultures.

ISSN:0003-2719    

DOI:10.1080/00032718208065146

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

On the basis of the spectral changes of indometnacin induced by changing the pH of the solvent medium, a method for its determination has been developed. The latter involves absorbance measurement of both acid and alkaline solutions of the compound at 260 nm: the difference between both values is linearly related to concentration in the range 0.4-1.4 mg/100 ml. The mean percentage recovery of authentic samples equal 100.1±0.42 (p=0.05). The method has been applied to the analysis of pharnaceutical preparations; the results obtained as compared to those of the official method indicate warranty of application in routine analysis.

ISSN:0003-2719    

DOI:10.1080/00032718208065147

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

The urinary androstanediol was determined in cases of idiopatic hirsutism by means of GLC. The determination was carried out on urines of 9 women before the beginning of a treatment with placebo and after three and six months of estrogen treatment. The initial range of values was between 39 and 99 μg/24h; at the end of the treatment the values ranged between 13 and 25 μg/24 h.

ISSN:0003-2719    

DOI:10.1080/00032718208065148

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

A glc method utilizing a flame ionization detector was developed for determining ethanol (I) in rabbit arterial serum. Addition of triton X-100 solution containing the internal standard, acetonitrile (II) to rabbit serum was followed by direct injection glc analysis using Porapak Q as the stationary phase. Ethanol was clearly separated from its major metabolite acetaldehyde (III) although the assay was not suitable for determination of III. Accuracy and precision of the method was demonstrated over the concentration range anticipated in acute intravenous dosing of I to rabbits. The method was evaluated in a rabbit which received three different intravenous infusion doses of I at two week intervals and found to be applicable to arterial serum analysis.

ISSN:0003-2719    

DOI:10.1080/00032718208065149

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

To study the human metabolism of bis (2-ethylhexyl)-phthalate (DEHP) urine samples were analyzed from non-uremic psoriatic patients, uremic patients undergoing hemodialysis treatments and patients undergoing cardiac bypass surgery using High Performance Liquid Chromatography (HPLC). The urine of dialyzed non-uremic patients contained phthalic acid, mono (2-ethylhexyl) phthalate and bis (2-ethylhexyl) phthalate. Other compounds identified were p-hydroxy benzoic acid, m-hydroxy benzoic acid, o-hydroxy hippuric acid, o-hydroxy benzoic acid and benzoic acid, which may be either diet dependent normal urinary constituents or metabolites of bis (2-ethylhexyl) phthalate. The levels of phthalic acid and bis (2-ethylhexyl) phthalate found in the urine of patients who were on total body oxygenators containing a membrane during cardiac bypass surgery were comparable to levels obtained from non-uremic psoriatic patients. Significant levels of phthalic acid were detected in the urine of the uremic patients studied while mono (2-ethylhexyl) phthalate and bis (2-ethylhexyl)-phthalate were present only in small amounts or were completely absent. In general, the urinary phthalate content of uremic patients increased with urinary volume.

ISSN:0003-2719    

DOI:10.1080/00032718208065150

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

Combining the rigidly controlable conditions of Flow Injection Analysis with detection by chemiluminescence, a method for determination of glucose in serum via enzymatic degradation by glucose in serum via enzymatic degradation by glucose oxidase is described. With a Standard deviation of less than 2% the sampling frequency is 75 samples per hour. Results obtained by the proposed procedure are compared with those acquired by a routine AutoAnalyzer method used at a local hospital. It is shown that any influence on the yield of chemiluminescence due to difference in viscosity of the injected samples effectively can be eliminated.

ISSN:0003-2719    

DOI:10.1080/00032718208065151

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

This is a scanned image of the original Editorial Board page(s) for this issue.

ISSN:0003-2719    

DOI:10.1080/00032718208065143

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor