摘要:

Reaction heats measured in a microcalorimeter between varying amounts of human serum and 700 IU of lipase (EC 3.1.1.3) and those between serum and 8 U of cholesterol oxidase (EC 1.1.3.6) were significantly linear with the contents of triglycerides (r=1.00) and cholesterol (r=0.99), respectively. However, when sera from 13 human volunteers were subject to comparative study between the micro-calorimetric and spectrophotometric methods, the correlation became less appreciable (r=0.70 and r=0.71) presumably due to the individual variations in free glycerol and cholesterol ester-constituents that were deliberately omitted in the microcalorimetric method but were routinely included in the standard (multi-enzymatic) spectro-photometric clinical method. The contribution of interfering substances, if any, is not totally ruled out.

ISSN:0003-2719    

DOI:10.1080/00032718208064395

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

A simple HPLC procedure is described for the determination of nine thiazides in the urine samples.

ISSN:0003-2719    

DOI:10.1080/00032718208064396

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

An enzymatic determination of urinary 7α-hydroxy bile acids is described. The principle of the method is as follows: after hydrolysis with β-glucuronidase and solvolysis, the ethyl acetate extract is washed with alkalin solution and water, then the alkali and water washes of the ethyl acetate extract are combined and the solution is acidified to pH 1 and sodium chloride added. Shake solution with ethyl acetate to re-extract the acidic fraction and the ethyl acetate layer is evaporated. Add enzyme color solution of 7α-hydroxysteroid dehydrogenase (7α-HSD, from E. coli) to a tube of extract residue and then after incubation, measure the absorbance of the solution.

ISSN:0003-2719    

DOI:10.1080/00032718208064397

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

The amount of copper, calcium, magnesium, sodium and potassium in reference serum were determined by integration of the signal. About 100 μ1 of the serum was diluted to 5 ml. Each 100 μ1 of this sample solution and standard solution was used for the determination. The results agreed well with the difinitive or recommended values.

ISSN:0003-2719    

DOI:10.1080/00032718208064398

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

Diazepam, oxazepam and N-desmethyldiazepam are determined by high performance liquid chromatography using a radially compressed C18 column and an aqueous methanolic mobile phase. The chromatographic separation is completed within 10 minutes. The drugs are recovered from serum by extraction with hexane:ethyl acetate 70:30, v/v.

ISSN:0003-2719    

DOI:10.1080/00032718208064399

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

N-(1-naphthyl) ethylene diamine dihydrochloride, thymol, α-naphthol and β-naphthol are used as coupling agents for the determination of diazotised procaine hydrochloride in pure form and in pharmaceutical preparations. All the experimental conditions have been adjusted, the effect of interferring substances, sensitivity and the validity of the procedure have been studied.

ISSN:0003-2719    

DOI:10.1080/00032718208064400

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor

摘要:

This is a scanned image of the original Editorial Board page(s) for this issue.

ISSN:0003-2719    

DOI:10.1080/00032718208064394

出版商:Taylor & Francis Group    

年代:1982

数据来源: Taylor