摘要:

Rapid detection of Staphylococcus aureus (S. aureus) using a separated electrode piezoelectric crystal sensor was reported in this paper. This method is simple, rapid and sensitive compared with other methods. Factors which affect determination were discussed and S. aureus over a range of 10–107cells/ml can be detected by proposed method.

ISSN:0003-2719    

DOI:10.1080/00032719508000316

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

摘要:

A sensitive and selective spectrophotometric determination of human serum albumin(HSA) was proposed by using the ternary complex-formation reaction among 2-(5-bromo-2-pyridylazo)-5-(N-phenyl-N-sulfopropylamino)phenol (5-Br.PAPS) as a pyridylazo derivative, cobalt(II) and HSA in the presence of poly(N-vinylpyrrolidone) (PVA) as a dispersion agent. The calibration curve was linear in the range of 0 - 7.0 μg/ml HSA by measuring the difference of absorbances at 636 nm between ((5-Br.PAPS)-cobalt(II)-HSA) and (5-Br.PAPS-cobalt(II)) solutions. The sensitivity was > 6-fold over the (Pyrogallol Red(PR)-molybdenum(VI)) method, and the recovery test in urine was satisfactory (97.5 ± 2.8%).

ISSN:0003-2719    

DOI:10.1080/00032719508000317

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

摘要:

The FIA-spectrofluorimetric determination of adrenaline was carried out by reaction of the drug with manganese dioxide entrapped in a polymeric material in a solid-phase reactor; the oxidized drug was monitored fluorimetrically at 540 nm (Ioxg. 330.0 nm). The calibration graph for adrenaline was linear over the range 0.5 - 20 μg ml−1with a relative standard deviation of 2.0% (at 5 ug ml−1) and the sample throughput of 65 h−1. The influence of foreign compounds was studied and the method was applied to the determination of adrenaline content in a pharmaceutical formulation.

ISSN:0003-2719    

DOI:10.1080/00032719508000318

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

摘要:

Hybrid hybridomas producing bispecific antibodies against α-subunit of hCG and Pd-coproporphyrin are obtained by using FACS. Conjugates of Pd-coproporphyrin with different proteins are tested for better sensitivity in micelle stabilized phosphorescent immunoassay. The role of steric factors for interaction of immobilized antibodies with antigens are discussed.

ISSN:0003-2719    

DOI:10.1080/00032719508000319

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

摘要:

γ-Aminobutyric acid (GABA) was determined by using flow injection analysis with a column of GABAse immobilized on glass beads. The NADPH produced was monitored fluorometrically. At a flow rate of 0.8 ml/min, peak heights were linear in the range 1-500 μM, and samples could be injected every minute. Nearly 100 % recovery of GABA was observed when GABA was added to infusions of green tea.

ISSN:0003-2719    

DOI:10.1080/00032719508000320

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

摘要:

Immunoaffinity chromatography (IAC) provides a selective method for sample preparation prior to high-performance liquid chromatography (HPLC) or gas chromatography (GC). Five different support materials were tested for their suitability with regard to IAC of herbicides (s-triazines). Three gels showing low (0-10 %) nonspecific s-triazine adsorption were used as affinity supports (Beaded cellulose ONB-carbonate A, LiChroprep hydrazide tentacle gel and LiChroprep azlactone gel). A monoclonal antibody (K4E7), directed against atrazine, was covalently coupled to the gels. High antibody immobilization rates (100 %) were obtained with the beaded cellulose gel in comparison to the other gels (60-70 %). Subsequently the IAC columns were loaded with triazine solutions. Glycine-HCl 0.2 mol/L, pH 2.2 yielded the best results for the elution of the bound triazines from the beaded cellulose IAC columns (70-100 %). Both the hydrazide tentacle and the azlactone IAC columns showed lower elution rates (60-80 %).

ISSN:0003-2719    

DOI:10.1080/00032719508000321

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

摘要:

A simple, rapid and selective high-performance liquid chromatographic (HPLC) method for the determination of sparfloxacin in human plasma has been developed and evaluated. Plasma protein was precipitated with acetonitrile. The drug and the internal standard (furosemide) were eluted from a Nova Pak C18cartridge column at 50°C with a mobile phase consisting of 5% acetic acid:acetonitrile:methanol (70:15:15% v/v). The column eluent was monitored at 364 nm. Each analysis required no longer than 5 min. Quantification was achieved by the measurement of the peak-area ratio of the analyte to the internal standard and the limit of quantification for sparfloxacin in plasma is 25 ng/ml. The intraday coefficient of variation (CV) ranged from 1.71% to 6.1%, and interday CV from 2.60% to 4.28% at four different concentrations. The absolute recoveries ranged from 98.6% to 104%, and the relative recoveries from 93.6% to 116.4% at four different concentrations. Preliminary stability tests showed that sparfloxacin is stable for at least 4-weeks in human plasma after freezing. The method is applied for the determination of the pharmacokinetic parameters of sparfloxacin after oral administration to 3 rats.

ISSN:0003-2719    

DOI:10.1080/00032719508000322

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

摘要:

A sensitive flow-injection spectrophotometric method has been developed for the determination of sodium nitroprusside (SNP). It is based on the fact that in alkaline medium, the concentration of the nitrite ions coming from the SNP corresponds with the initial quantity. The nitrite ions are quantified spectrophotometrically by diazotizing with sulphadiazine in acid medium and then coupled with N-(1-naphthyl)ethylenediamine dihydrochloride (NEDA) to give a purple coloured azo dye measured at λmax545 nm. Beer's law is obeyed over the concentration range 0.1-100 μg/ml and the precision (as % RSD) is 1.78% (n=10) for 10 μg/ml. The limit of detection being 0.05 μg/ml (defined as the amount of the drug that gave a signal of twice the back-ground noise). The sample throughput is 17.5 samples h−1. The optimized reagents concentrations as well as the flow injection (FI) parameters were investigated. The applicability of the method to determination of SNP was demonstrated by investigating the effect of potential interferences and by analysis of a commercial pharmaceutical preparation.

ISSN:0003-2719    

DOI:10.1080/00032719508000323

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

摘要:

Room-temperature phosphorescence (RTP) emission was detected from several organic substrates using a solid surface composed solely of alkali salts. This technique yields detection limits comparable to the popular polymer-salt and filter paper methods. More importantly, the alkali halide matrix environment requires no special drying processes, has a simple, reproducible sample preparation procedure, and yields no interfering background RTP.

ISSN:0003-2719    

DOI:10.1080/00032719508000324

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor

摘要:

The operating conditions for the spectrophotometric determination of selenium(IV) with the sodium salt of hexamethyleneiminecarbodithioate (NaHMICdt) as a ligand by liquid-liquid extraction technique are presented. In acidic conditions selenium forms a yellow complex with the ligand which can be extracted in toluene with an absorption maxima at 335 nm. The molar absorptivity is 4.42 × 1031 mol−1cm−1and the Sandell's sensitivity is 16.6 ng cm−2. The difference in the absorbance between the blank determination and selenium(IV) sample increases linearly in the concentration range 0.5-4.0 mg/L at pH 1.0 and 4N HCl. The stoichiometry of the complex was found to be 1:4. The proposed method is extremely sensitive, reproducible and has been satisfactorily applied to the determination of trace amount of selenium in polluted water, plant material, wheat flour and medicated shampoo.

ISSN:0003-2719    

DOI:10.1080/00032719508000325

出版商:Taylor & Francis Group    

年代:1995

数据来源: Taylor