摘要:

An ion-pair HPLC approach with ordinary silica has been applied, with detection by ultraviolet absorption, to the assay of plasma for chlorpromazine and its sulfoxide on the one hand, and for 7-hydroxychlorpromazine (an active metabolite) on the other hand. The respective sample-preparation procedures entail extraction of the plasma with heptane at strongly alkaline pH, or else with diethyl ether at a less alkaline pH and with ensuing back-extraction and re-extraction. For each of the compounds, levels as low as 10 ng.ml−1are measurable. The conditions adopted are such that specificity and reproducibility are satisfactory although chlorpromazine and its various metabolites, especially 7-hydroxychlorpromazine, are chemically unstable and, moreover, are readily lost onto glass. With the unorthodox separation system adopted, adsorption rather than partition appears to be the dominant mechanism.

ISSN:0003-2719    

DOI:10.1080/00032718108055479

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

A direct nonaqueous potentiometric determination of glaphenine hydrochloride and glaphenine authentic compounds and in their pharmaceutical formulations, namely glifanan suppositories, glifanan and adalgur tablets(R)is developed. The method involves the titration with 0.1N aceteous perchloric acid after the addition of mercuric acetate in case of hydrochloride salts, using a combined glass electrode. The results of the method were compared with those of the spectrophotometric1and the alkalimetric2procedures. The method can be adopted for quantities ranging between 50 and 500 mg with an accuracy of 102 ± 1.1, 98.8 ± 0.4 and 101.2 ± 0.5 for glifanan suppositories, glifanan tablets and adalgur tablets respectively.

ISSN:0003-2719    

DOI:10.1080/00032718108055480

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

Extraction of steroids and steroid conjugates with Amberlite XAD-2 and Sep-Pak C18has been studied. Amberlite XAD-2 has ionic sites with high affinity for conjugated steroids, particularly steroid disulphates. Nearly quantitative recoveries of steroid conjugates are obtained when the resin is washed with an aqueous solution of a sulphate prior to elution with methanol. Such treatment is not required using Sep-Pak C18cartridges.

ISSN:0003-2719    

DOI:10.1080/00032718108055481

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

A simplified procedure for selective isolation of ethynyl steroids from urine is described. Urinary steroids are extracted with Sep-PakRC18and subjected to enzyme hydrolysis. The liberated steroids are extracted with Lipidex 1000 and the eluate from this column is passed through a bed of sulfohydroxypropyl Sephadex LH-20, partly converted into silver form by a wash with aqueous silver nitrate. Ethynyl steroids are eluted with a solution of acetylene in methanol.

ISSN:0003-2719    

DOI:10.1080/00032718108055482

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

The design and operation of a double or bipotentiostat used to independently cont.ro1 the potential and measure the current produced at two working electrodes in a four-electrode thin-layer electrochemical detector, is described.

ISSN:0003-2719    

DOI:10.1080/00032718108055483

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

The stability of uric acid in alkaline solutions has been studied by spectrophotometric, enzymic and chemical methods. Aqueous triethanolamine was shown to maintain stability of uric acid for a range of concentrations at around 2O°C and to considerably reduce the degradation loss at the higher temperature of 37°C.

ISSN:0003-2719    

DOI:10.1080/00032718108055484

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

ISSN:0003-2719    

DOI:10.1080/00032718108055485

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

ISSN:0003-2719    

DOI:10.1080/00032718108055478

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor