摘要:

A dot-immunobinding assay for cytomegalovirus antibody in serum has been developed using a nitrocellulose sheet as the solid support and a protein A-horseradish peroxidase conjugate. Bound conjugate was detected using a para-iodophenol enhanced luminescent assay. Light emission from the glowing dots on the membrane were recorded using high speed instant photographic film (ASA 20,000). Photographic results of CMV antibody assays were assessed visually by comparison with results of standards run on each membrane.

ISSN:0003-2719    

DOI:10.1080/00032718508066212

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

Integration methods for measuring substrates via homogeneous immunoassays are presented along with their theoretical bases. Integrals of differences in measured responses over time are used with enzyme multiplied immunoassay techniques and show unique relationships between the net integral data and analyte concentrations. Changes in indicator response signals are integrated over several minutes for drugs of forensic interest, and results for integrations of both published response curve data and laboratory assays are evaluated.

ISSN:0003-2719    

DOI:10.1080/00032718508066213

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

A Simpler method for the preparation of monomeric affinity-purified Fab'-ß-D-galactosidase conjugate is described. Rabbit (anti-human IgG) serum was subjected to successive processes of pepsin digestion to convert IgG to F(ab')2′reduction with 2-mercaptoethy on a column of human IgG-Sepharose 4B. The affinity-purified Fab' thus obtained without using gel filtration was reacted with excess of maleimide groups introduced into ß-D-galactosidase fromEscherichia coli.The monomeric Fab'-ß-D-galactosidase conjugate formed was separated from unconjugated Fab' by gel filtration and from unconjugated ß-D-galactosidase by affinity chromatography on a column of goat (anti-rabbit IgG) IgG-Sepharose 4B. By immunoenzymometric assay technique for human IgG, the monomeric conjugate was compared with a monomeric conjegate prepared by a previously reported complexmethod and non-monomeric conjugate which contained 3.7 Fab' molecules per ß-D-galactosidase molecule. The present monomeric conjugate provided as sensitive a dose-response curve as the previously reported monomeric conjugate and a more sensitive dose-response curve than the non-monomeric conjugate.

ISSN:0003-2719    

DOI:10.1080/00032718508066214

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

A sensitive and reliable liquid chromatographic assay procedure for the quantitation of oxamniquine in plasma or urine was developed. Chromatographic separation was achieved on a reversed-phase phenyl colum using U.V. Detection at 254 nm. The eluting solvent was the mixture of 0.05 M acetate buffer pH 5 and acetonitrile (3:7). With this mobile phase the drug and its external standard were well separated from the interference of the blank samples. The average recovery of oxamniquine from 3 or more replicate dog plasma samples of different concentration (0.125 − 4.00 μg/ml) was 95.5% and its coefficient of variation was 4.17%. The reproducibility of the assay was confirmed by the analysis of variance test for the slopes of the three standard plots obtained from plasma samples at three different occasions (F=4.2, p > 0.01). The detection limit for plasma samples was approximately 20 ng/ml. The method was applied to measure the plasma level vs, time profile of this drug following a single bolus intravenous dose of 16 mg/kg to a dog.

ISSN:0003-2719    

DOI:10.1080/00032718508066215

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

A Kinetic method is based on the degradation rate of tetracycline (TC) or oxytetracycline (OTC) to their anhydro derivatives with subsequent spectrophotometric measurements. With optimized degradation rate parameters, calibration curves relating decrease in absorbance difference (of alkaline solution versus acid solution) as a funotion of concentration in a range of 0.8-4.0 mg% are linear with negligible interecept. The method is applicable for powder and capsule assays and gives results within the pharmacopoeial limits.

ISSN:0003-2719    

DOI:10.1080/00032718508066216

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

The first derivative curve (D1) of absorption spectrum of Phenytoin in buffer pH 10 develos negative peaks at 244, 263 and 270 nm. D1at 244 nm was found linearly related to concentration over a range 0.4 − 1.4 mg per 100 ml and highly reproducible (C.V. %=0.62). Tablets and capsules have been analyzed using D1at 244 nm and the mean percentage found were 99.7 ± 0.81 and 103.3 ± 0.44, respectively. The B.P. method gave 100.9 and 103.8 %, respectively.

ISSN:0003-2719    

DOI:10.1080/00032718508066217

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

Conductometric titrations of metformin and moroxidine are reported. The procedure is based on the copper-biguanide reaction which gives a pink soluble complex. Studies at several pH values and presence of NH3are carried out. NaOH-NH3(1:15) are added to 5 ml of biguanide aqueous solution and diluted to 60 ml and titrated with copper sulphate. Concentrations of metformin in 205x10−4-1,7x10−3M range are determined. Foreign species presence is studied too.

ISSN:0003-2719    

DOI:10.1080/00032718508066218

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

A simple and sensitive spectrophotometric method is described for the assay of some tranquillizers and antidepressants, namely, chlorpromazine, imipramine, amitriptyline and chlorprothexine. The method was based on the interaction of these drugs as n-electron donors with 2,3-dichloro-5,6-dicyano-p-benzoquinone (DDQ) as λ -acceptor to give a highly coloured radical anion which exhibits maximum absorption at 460 nm. The radical anion was proved by electron spin resonance measurements. The proposed method has been successfully applied for the determination of the examined drugs in tablets. The assay results were in accord with the pharmacopoeial results.

ISSN:0003-2719    

DOI:10.1080/00032718508066219

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

Eosin reacts with phenothiazine derivatives in dichloroethane to give intense fluorescent compounds having their excitation maximum at 318 nm and emission maxima at 450, 460 and 465 nm for prochlorperazine dimaleate, thiethylperazine dihydrochloride and trifluoperazine dihydrochloride, respectively. Under the optimum conditions, samples of 1-10 μg ml−1could be determined rapidly with coefficient of variation less than 1.8 %. This fluorescence reaction was also applied successfully to the determination of phenothiazine drugs in some pharmaceutical preparations.

ISSN:0003-2719    

DOI:10.1080/00032718508066220

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor

摘要:

A sensitive spectrophotometric method for the determination of three kdetosteriod drugs is suggested. The method is based on oximation, separation and acid cleavage of their oximes followed by spectro-photometric measurement of the liberated hydroxylamine through oxidation and diazo coupling reactions. The method gave mean percent recoveries of 98.92 ± 0.58, 99.26 ± 0.96, and 99 10 ± 0.47 for progesterone, testosterone propionate and nandrolone phenyl propionate respectively in ampoules. Results were in good agreement with B.P. 1980 method.

ISSN:0003-2719    

DOI:10.1080/00032718508066221

出版商:Taylor & Francis Group    

年代:1985

数据来源: Taylor