摘要:

A spectrofluorimetric method for protein estimation based on the use of l-4-diamino-2-3-dich1oro-anthraquinone as a fluorogenic reagent is presented and applied to renin study. The method is optimized and the variables that influence the development of the fluorescence are studied. Data obtained by this method for Renal Renin Activity (RRA) are compared with those obtained by Bioassay (BA) and Radioinmunoassay (RIA), finding that this spectrofluorimetric method is a good one related to Radioinmunoassay.

ISSN:0003-2719    

DOI:10.1080/00032718108059831

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

A highly sensitive sandwich enzyme immunoassay (EIA) for human ferritin was developed using rabbit anti-ferritin IgG-coated polystyrene balls and affinity-purified rabbit anti-ferritin Fab' labelled with β-D-galactosidase fromEscherichia coliand compared with the corresponding sandwich radioimmuno assay (RIA). The specific and nonspecific binding of labelled anti-ferritin to the polystyrene balls were examined in relation to the amount of labelled anti-ferritin used per tube, and the highest sensitivity of each immunoassay (0.2 amol/tube in EIA and 2.5 amol/tube in RIA) was obtained by using the minimal amount of the corresponding labelled anti-ferritin (0.71 fmol in EIA and 4.5 fmol=4436 cpm in RIA) which gave a reliable calibration curve. The sandwich RIA was less sensitive, largely because the specific radioactivity of125I-labelled anti-ferritin used was not sufficiently high.

ISSN:0003-2719    

DOI:10.1080/00032718108059832

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

High performance thin layer chromatography (HPTLC) under controlled relative humidity conditions was employed in order to separate phospholipids of amniotic fluid. Quantitative determination was accomplished after visualization by copper acetate/phosphoric acid (CA) and by phospho-molybdic acid (PMA). In both cases reflectance was found to follow the Kubelka-Munk theory in the Treiber and Pollak approximation. PMA gives fairly better results as it concerns reproducibility. A method of analysis is proposed that allows to use 500 μl or less of amniotic fluid. A complete phospholipid analysis can be performed in 40 min. Proposed method was tested on simulated and real samples and results compared with conventional spectrophotometry analysis.

ISSN:0003-2719    

DOI:10.1080/00032718108059833

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

The effect of various soft acid cation acetate salts on the retention of the peptides Phe-(Gly)n, where n=1–4, and on phenylalanine, indicates a correlation between the softness character of the cation of the acetate salt used in the mobile phase and retention in reversed-phase high performance liquid chromatography. Inorganic soft acid cation salts did not exhibit the same increased retention properties. By using silver(I) valerate or silver(I) octanoate as ion-pairing reagents in the mobile phase for RP-HPLC, static retention of peptides was achieved.

ISSN:0003-2719    

DOI:10.1080/00032718108059834

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

A new simple and fast micro method has been established to determine the optical purity of a small amount of amino acids (0.1–1 μg/ml). The amino acids react readily with fluorescamine to form pyrrolinone-type chromophores, which show strong CD bands at 400–300 nm and much larger optical rotation than the free amino acids in the visible spectral range.

ISSN:0003-2719    

DOI:10.1080/00032718108059835

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

A simple and specific method for the assay of cephalexin in human serum by high performance liquid chromatography is described. Cephalexin was extracted from serum with 5 fold volume of methanol and chromatographed on a reversed-phase column using 30 % aqueous acetonitrile solution containing 0.005 M sodium 2-propanesulfonate (pH 3.0) as the mobile phase. Cephalexin was detected by the absorbance at 254 nm. Only 20 μl of serum was required and all of the operations for analysis were completed within 10 min. This method was proved to be effective in the rapid monitoring of serum cephalexin concentration in humans who received its ordinal and long-acting preparations.

ISSN:0003-2719    

DOI:10.1080/00032718108059836

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

A chemical ionization and an electron impact GC/MS assaying approach is presented for determining dibucaine concentrations in biological fluids. Both use deuterium-labeled drug as the internal standard and rely on the same sample extraction and sample preparation procedure. Under chemical ionization conditions (CH4), the assaying limits are in the range of 1–80 ng/ml of serum. Under electron impact conditions, the analytical range is 20–800 ng/ml. The chemical ionization procedure has been found suitable for monitoring drug levels in man. One volunteer, who received a single 5-mg oral dose, showed peak serum drug concentrations of 23 ng/ml attained 2 hr after drug administration. The biologic half-life (ß phase) was 11 hr.

ISSN:0003-2719    

DOI:10.1080/00032718108059837

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

A unique detector for a specific ionic drug has been fabricated. This sensor has been used to quantitatively determine naproxen in either tablet or capsule form. Detector sensitivity for naproxinate ion was ≤ 10−5M. with very good selectivity in the presence of most inorganic ions. Assay times were short and appeared amenable to automation.

ISSN:0003-2719    

DOI:10.1080/00032718108059838

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

摘要:

Authors propose a simple method for U. V. determination of blood paracetamol in emergency toxicology. First, diethy1-ether extraction allows salicylate elimination (especially salicylic acid which is the most frequently used in therapy). Secondly paracetamol is extracted by ethyl-acetate and measured by U. V. spectrophotometry at 244 nm. The method provides good repeta-bility (C V=3.3 %) and reproducibility (C V u 4.3 %) with a detection limit of 2 mg L−1in plasma.

ISSN:0003-2719    

DOI:10.1080/00032718108059839

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor

ISSN:0003-2719    

DOI:10.1080/00032718108059840

出版商:Taylor & Francis Group    

年代:1981

数据来源: Taylor