摘要:

Ceric sulphate ie applied to the determination of ephedrine HCL and norephedrine HCl. The method depends upon titrimetric determination of these amines with ceric-sulphate In acid medium using ferroin as indicator.

ISSN:0003-2719    

DOI:10.1080/00032718408077182

出版商:Taylor & Francis Group    

年代:1984

数据来源: Taylor

摘要:

Chloranil (2,3,5,6-teterachloro-p-benzoquinone) reacts with certain aminoglycosoide antibiotics in borate buffer at pH 9.0 to give charge-transfer complexes. The complexes possese an absorption maxmum at about 350 nm with molar absorptivities of 3.78 × 105, 4.69 × 105, 5.0 × 105and 7.08 × 105for kanamycin, neomycin B, amikacin and tobramycin reepectively, These high intensity of the chargetransfor band and confirmity to beer's law enabled assay of these antibiotics with appreciable sensitivity and good precision.

ISSN:0003-2719    

DOI:10.1080/00032718408077183

出版商:Taylor & Francis Group    

年代:1984

数据来源: Taylor

摘要:

Six commercially available solid adsorbent materials were evaluated for their applicability as effective tools for extraction of drugs from aqueous and human plasma samples. Ten model compounds were selected as representative of the acidic, basic, amphoteric, hydrophobic, and hydrophilic drug classes. Percent recovery and precision data for each model drug on each solid adsorbent material were calculated using spiked water and/or plasma samples. Octadecylsilane materials were the best overall choice for extraction of the different chemical classes of drugs from water and plasma samples, giving both highest recovery and best reproducibility data for a majority of the drugs studied. It was also determined that good extractability for basic drugs could be obtained using either XAD-2, Clin-eht® or cyanopropyl columns. In addition, XAD-2 gave good recoveries of amphoteric compounds and Clin-elut® was good for hydrophobic drugs.

ISSN:0003-2719    

DOI:10.1080/00032718408077184

出版商:Taylor & Francis Group    

年代:1984

数据来源: Taylor

摘要:

A fluorometric method of Tb-SSA-EDTA-surfactant complex for determining salicylic acid in serum and urine is proposed. This method is fast, simple, sensitive in determining sallicylate ion in aqueous medium. Protein removal from serum is not required. 1.0 × 10−-4M- 1.0 × 10−-2M of salicylic acid in samples can be determined by the proposed procedure. The percentage of recovery was satisfactory. This procedure can be used in emergency toxicology.

ISSN:0003-2719    

DOI:10.1080/00032718408077185

出版商:Taylor & Francis Group    

年代:1984

数据来源: Taylor

摘要:

Primaquine in a flowing solvent is photolyzed with a 200 W Xe-Hg arc lamp and the intensely fluorescent photochemical product is measured in a fluorimeter. The effect of solvent composition and pH, irradiation time, and excitation conditions are evaluated. Primaquine in blood serum is measured by introducing a sample of blood serum into the flowing stream and performing the photochemical-fluorescence measurement. The fluorescence background of blood serum was very low allowing a detection limit of less than 0.1 μg/mL. Excellent recoveries were obtained.

ISSN:0003-2719    

DOI:10.1080/00032718408077186

出版商:Taylor & Francis Group    

年代:1984

数据来源: Taylor

摘要:

The separation of the three major isomers and the main photoproducts of bilirubin by Ion-Pair High Pressure Liquid Chromatography is described. Chromatographic procedures found in the literature proved difficult to reproduce, had unacceptably long elution times (over 2 hours), required complex gradients or two or more columns. As these reports provided no data to show why specific chromatographic conditions were chosen an optimization study was carried out. The separation is shown to be dependent on pH, organic content and buffer concentration of the mobile phase, ion-pairing agent concentration and injection solvent. A separation, carried out in less than thirty minutes on octadecyl silica using isocratic conditions with detection at 455 nm is demonstrated. This technique will be used in the elucidation of the mechanism involved in phototherapy treatment of neonatal jaundice.

ISSN:0003-2719    

DOI:10.1080/00032718408077187

出版商:Taylor & Francis Group    

年代:1984

数据来源: Taylor

摘要:

A liquid chromatographic differential refractive index detector (RI) and an ultraviolet absorption (at 195 nm) detector (UV) were studied for detection of triglyceride (TG) and phospholipid (PL) molecular species. It was found that the log UV/RI detector response ratio was linearly related to the log of the number of conjugated double bonds (degree of unsaturation) of the lipid in a predictable fashion, indicating the usefulness of the combination of both detectors in TG and PL analysis. The detection limit of the RI detector for TG and PL was found to be 5 × 10−7M or as sensitive as the UV detector for saturated TG and PL.

ISSN:0003-2719    

DOI:10.1080/00032718408077188

出版商:Taylor & Francis Group    

年代:1984

数据来源: Taylor

摘要:

The electrochemical activity determination of γ-glutamyl-transpeptidase is described using the electrogenic L-γ-glutamyl paraaminodiphenylamide substrate. In order to correlate the results with classical measurements involving chromogenic substrates, linear calibration curves for the amperometric detection of the released free amine, the kinetics of the catalysis and the enzyme level in one plasma sample have been compared to photometric experiments effectuated in parallel runs, in the same conditions, with L-y-glutamyl paranitroanilide as the substrate. Results show that this new technique applies successfully to such evaluations.

ISSN:0003-2719    

DOI:10.1080/00032718408077189

出版商:Taylor & Francis Group    

年代:1984

数据来源: Taylor

摘要:

A biometrical study carried out on 50 sera, concerning relationships between ELISA 31-13 passive hemagglutination (PHA), both previously standardized against the neutralization test (NT) for the evaluation of antitetanic imunity level, is described, The statistical analysis of intermethods regression and of variance allowed for the estimation of the immunological proteation limit established at a 0.3 IU/ml value (upper confidence limit, p ⩽0.05) in terns of PHA, corresponding to 0.015 IU/ml in terms of ELISA at the same probability level, The correlation coefficient was 0.94, The precision of ELISA was significantly higher in comparison with those of NT and PHA The reasons for a selective choice of the proper type of test in various circumstances are discussed.

ISSN:0003-2719    

DOI:10.1080/00032718408077190

出版商:Taylor & Francis Group    

年代:1984

数据来源: Taylor

摘要:

Amino acid esters and amides fail to produce strong fluorescence following their reaction witho-phthalaldehyde (OPA) in the presence of β-mercaptoethanol. The lack of luminescence appears to arise from a quenching phenomenon rather than from reduced reactivity of these substrates toward OPA. The amido hydrogen in the peptide linkage has been implicated in the loss in fluorescence yield.

ISSN:0003-2719    

DOI:10.1080/00032718408077191

出版商:Taylor & Francis Group    

年代:1984

数据来源: Taylor