摘要:

This report describes the simultaneous radioimmunoassay of plasma testosterone (T) and 5a-dihydrotestosterone (DHT), using as binding reagent a highly specific antiserum which reacted significantly with T and DHT. Utilizing a one step celite microcolumn to separate T from DHT, we have measured these two androgens accurately in small aliquots of plasma from both male and female subjects, Plasma concentrations of T and DHT expressed as mean ± S.D. in ng/ml were respectively:1) in male subjects:a. normal adult: 4.9 ± 1.6 (T) and 1.3 ± 0.8 (DHT); b. hypogonadal: 0.24 ± 0.08 (T) and 0.20 ± 0.10 (DHT); c. prepubertal: 0.14 ± 0.02 (T) and 0.05 ± 0.02 (DHT).2) in female subjects:a. normally menstruating, irrespective of time of cycle: 0.22 ± 0.07 (T) and 0.34 ± 0.17 (DHT); b. postmenopausal: 0.29 ± 0.08 (T) and 0.15 ± 0.07 (DHT); c. ovariectomized: 0.24 ± 0.06 (T) and 0.09 ± 0.01 (DHT). When plasma levels of T and DHT were measured during a complete menstrual cycle, the levels of these steroids were found to be higher during the luteal than during the follicular phose. The ratio of plasma T:DHT concentrations was five times higher in normal adult male (3.1) than in menstruating female subjects (0.6).

ISSN:0003-2719    

DOI:10.1080/00032717208066108

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

Plasma or serum is diluted ten times with 0.1 M NaOH. Bile acids are extracted and purfied by passing the sample through a column of Amberlite XAD-2. The resin adsorbs the bile acids, which are then eluted with ethanol. After addition of sodium hydroxide and hydrolysis, the free bile acids are extracted by Amberlite XAD-2 and are then methylated and analyzed by gas chromatography. The degree of purification obtained in the first extraction step is sufficient to permit an analysis of conjugated bile acids or bile acid sulfates by thin-layer or Sephadex LH-20 chromatography.

ISSN:0003-2719    

DOI:10.1080/00032717208066109

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

O-Butyloximes and O-pentyloximes are useful derivatives for gas chromatography of steroids, by virtue of the convenient increments in retention time accompanying their formation from reactive keto-steroids. These increments are adequate to permit analytical separations of keto- from hydroxysteroids, but are also moderate enough to allow the elution of steroidal di-O-alkyloximes at normal operating temperatures. The molecular weights of all the derivatives lie in a range very suitable for mass spectrometry, and the fragmentation modes are informative.

ISSN:0003-2719    

DOI:10.1080/00032717208066110

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

An initial investigation of a pulsed hollow cathode lamp has been made to determine its advantage in decreasing the limit of detection in atomic fluorescence flame spectrometry. From the results obtained with the pulsed system utilizing a boxcar integrator readout, it can be concluded that pulsed hollow cathode lamps provide a greatly improved signal-to-noise ratio as compared with the conventional hollow cathode system.

ISSN:0003-2719    

DOI:10.1080/00032717208066111

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

A method has been developed at the Oak Ridge Y-12 Plant1that will determine the percentage quantities of boron in borosilicate glass by flame emission spectroscopy. The sample is dissolved in hydrofluoric acid, nebulized into a nitrous oxide-acetylene flame, and a measurement made of the BO2band emission at 5180 angstroms. Glass containing at least 1.0 weight percent boron can be analyzed. The relative standard deviation of the method is 5.1 percent. Approximately 10 samples (2 aliquots each) can be analyzed in four hours.

ISSN:0003-2719    

DOI:10.1080/00032717208066112

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

Four different transport mechanisms can be applied for substance transport in TAS-procedure. Propellents are of considerable importance in “Jet” -distillation or -sublimation. The special aptitude of neutral, basic and acid propellents has been thermogravimetrically tested. Molecular sieve 4Å, saturated with water, hexamminenickel (II) chloride and malonic acid have proved to be especially favourable in comparison to the propellents employed hitherto.

ISSN:0003-2719    

DOI:10.1080/00032717208066113

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

“Low Temperature Ashing” pretreatment of natural carbonate ores suppressed interferences due to oxidizable organic impurities on DTA curves. Experiments involving calcite, dolomite, ankerite, siderite, rhodocrosite and spherocobaltite indicated that the basic DTA patterns were not adversely affected by the pretreatment outlined in this paper.

ISSN:0003-2719    

DOI:10.1080/00032717208066114

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

Kinetics of the dissociation of ethy1 N-hydroxymethylcarbamate, and monomethylolethylcarbamate (MMEC) at various temperatures and pH values have been studied by measuring the formaldehyde concentration in solution via an electrochemical technique. Formaldehyde and ethyl carbamate (EC) are the products of the dissociation. Formaldehyde concentration as a function of time has been used in a computer program designed to calculate the rate constant, kf, for the formation of MMEC form formaldehyde and EC and the rate constant, kr, for the dissociation of MMEC.

ISSN:0003-2719    

DOI:10.1080/00032717208066115

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor

摘要:

This is a scanned image of the original Editorial Board page(s) for this issue.

ISSN:0003-2719    

DOI:10.1080/00032717208066107

出版商:Taylor & Francis Group    

年代:1972

数据来源: Taylor