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1. |
A Simple Electrochemical Method for the Quantitative Determination of Acetaminophen in Serum |
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Analytical Letters,
Volume 14,
Issue 13,
1981,
Page 1003-1012
Andrew Falkowski,
Robert Wei,
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摘要:
A simple electrochemical method for the determination of acetaminophen in serum is described. The eleotrode and associated electronics are simple, reliable, and inexpensive to build. The apparatus can be operated at a rate of 2–3 determinations per minute using only 10 μl serum per determination. The procedure includes extraction of acetaminophen in ethyl acetate and subsequent oxidative amperometric detection of the drug by a form of flow-injection analysis. The system parameters of buffer, pH, and redox potential have been optimized to permit measurement of less than 10 μg/ml of acetaminophen. The determination is linear over the range of 10–300 μg/ml with a C.V. of less than 3% for replicate analysis of the same sample.
ISSN:0003-2719
DOI:10.1080/00032718108081444
出版商:Taylor & Francis Group
年代:1981
数据来源: Taylor
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2. |
An HPLC Procedure for the Analysis of Furosemide in Pharmaceuticals-Analysis of Furosemide Tablets and Furosemide Injection |
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Analytical Letters,
Volume 14,
Issue 13,
1981,
Page 1013-1030
Jeri Roth,
RaoS. Rapaka,
VadlamaniK. Prasad,
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摘要:
A simple and specific procedure was developed for the analysis of furosemide from tablets and injections. The procedure consists of extracting furosemide into aqueous sodium hydroxide, addition of the internal standard, appropriate dilution and injection onto a u Bondapak C18reversed phase column. The mobile phase consisted of a solvent containing acetonitrile and aqueous sodium acetate and the eluate was monitored by either U.V. absorption or spectrofluorimetry. A standard linear calibration curve was obtained for direct standard solutions containing 75 ng to 500 ng on column. This procedure was successfully used to analyze furosemide tablets (individual assay) and injections.
ISSN:0003-2719
DOI:10.1080/00032718108081445
出版商:Taylor & Francis Group
年代:1981
数据来源: Taylor
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3. |
Sodium Coumarin 6-Sulfonate, A Fluorometric Ion-Pairing Reagent for Tertiary Amines. Application to the Determination of Chlorpheniramine Maleate |
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Analytical Letters,
Volume 14,
Issue 13,
1981,
Page 1031-1046
LemseulL. Dent,
JamesT. Stewart,
IrwinL. Honigberg,
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摘要:
A study of sodium coumarin 6-sulfonate as a fluorescent ionpair reagent indicated that it could be useful in the analysis of tertiary amines such as chlorpheniramine maleate. The physical properties of the coumarin sulfonate that make it suitable as a fluorescent ion-pair reagent for basic drugs are its high relative quantum yield (0.76) and its acidity (pKa of −7.66). Methylene chloride containing 5%n−pentanol was used to extract the coumarinchlorpheniramine ion-pair from aqueous solution. It was found that a 10−2Mcoumarin concentration yielded a 92% recovery of chlorpheniramine at pH 5. Following phase separation, the coumarin species was completely ionized by the addition of tetrabuty1 ammonium hydroxide to the organic phase. After irradiation for 30 min using long wavelength ultraviolet light (365 nm), the fluorescence intensity of the sample was measured using excitation and emission wavelengths of 400 and 540 nm, respectively. Comparison of fluorescence data of spiked aqueous samples to that of a chlorpheniramine maleate standard curve performed concurrently gave drug concentration in the samples. The calibration curve was linear in the 50–1000 ng/ml range (0.13 − 2.6 × 10−6M). The procedure allowed the determination of chlorpheniramine maleate with an accuracy of 4–6% and a precision of 2–6% RSD (relative standard deviation). The minimum detectable concentration of drug (S/N = 2) that can be assayed by this method is 50 ng/ml of the maleate salt (35.3 ng/ml of chlorpheniramine free base).
ISSN:0003-2719
DOI:10.1080/00032718108081446
出版商:Taylor & Francis Group
年代:1981
数据来源: Taylor
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4. |
Application de la Polarographie Sinusoidale au Dosage Sanguin de L'indomethacine |
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Analytical Letters,
Volume 14,
Issue 13,
1981,
Page 1047-1070
M. Alkayer,
J.J. Vallon,
Y. Pégon,
C. Bichon,
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摘要:
Alternative current polarography has been applied to indomethacin determination in serum, after dichloromethan extraction at pH 3.0. Solvent is evaporated and the residue submited to hydrolysis by NaOH. The indole derivative obtained is nitrosated by nitrous acid at pH 3,5. The indole-nitrosamine formed is measured by polarography at pH 1,3 and compared with an added quantity of the pure nitrosated product. The proposed method is selective and sensitive; it allows measurement of concentrations in serum as low as o,30 μg ml−1(0.83. 10−9moles ml−1).
ISSN:0003-2719
DOI:10.1080/00032718108081447
出版商:Taylor & Francis Group
年代:1981
数据来源: Taylor
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5. |
Development of a Laser Nephelometric Method for the Quantitation of Human Glycohemoglobins |
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Analytical Letters,
Volume 14,
Issue 13,
1981,
Page 1071-1087
PeterP. Chou,
Julius Kerkay,
ManjulaK. Gupta,
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摘要:
A nephelometric procedure for quantitative measurement of glycohemoglobin (Hb A1) was developed, evaluated, and compared with the semi-quantitative mini-column chromatographic procedure. Hb A1was purified from human red cell hemolystate by Bio-Rex 70 ion-exchange liquid chromatography and was used for standards and immunization. The antisera raised in rabbits showed high cross-reactivities with normal human hemoglobin (Hb A). The latter was separated by mixing 25 μl of patients' hemolystate with 2 ml ion-exchange resin suspension for 15 minutes. One hundred microiters of the supernatant was incubated with 900 μl antiserum (dilution 1:50) for 45 minutes at room temperature. Samples were then read on a laser nephelometer. The sensitivity of the assay was found to be 0.1 mg Hb A1. The intraassay relative standard deviation (RSD) was 5.6% and the interassay RSD was 6.5%.
ISSN:0003-2719
DOI:10.1080/00032718108081448
出版商:Taylor & Francis Group
年代:1981
数据来源: Taylor
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6. |
Cadmium, Copper, Lead, Manganese, and Selenium Levels, and Glutathione Peroxidase Activity in Human Kidney Cortex |
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Analytical Letters,
Volume 14,
Issue 13,
1981,
Page 1089-1109
PatriciaA. Pleban,
Julius Kerkay,
KarlH. Pearson,
Julius Kerkay,
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摘要:
Cadmium, copper, lead, manganese, and selenium concentrations and glutathione peroxidase (EC 1.11.1.19) activity have been determined in human kidney cortex specimens obtained at autopsy. Trace metal concentrations for each specimen were determined in the same digest, while glutathione peroxidase activity was assayed in tissue collected at a site adjacent to that selected for the trace metal determinations. Glutathione peroxidase activities were determined with two substrates, hydrogen peroxide and t-butylhydroperoxide. The effects of age, smoking history, sex, race, and blood pressure on kidney cortex trace metal levels and glutathione peroxidase activity were investigated. Mean kidney cortex cadmium concentration was found to be significantly higher in smokers than in nonsmokers (p=0.010). In addition, the mean kidney cortex lead concentration of hypertensive individuals was found to be significantly higher than that of non-hypertensive individuals (p=0.017), Glutathione peroxidase activities utilizing hydrogen peroxide substrate were inversely correlated to kidney cortex manganese levels (p=0.004).
ISSN:0003-2719
DOI:10.1080/00032718108081449
出版商:Taylor & Francis Group
年代:1981
数据来源: Taylor
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7. |
Preliminary Evaluation of Mixed Dyes for Fingerprinting Non-Fluorescent Bacteria |
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Analytical Letters,
Volume 14,
Issue 13,
1981,
Page 1111-1124
DennisC. Shelly,
JohnM. Quarles,
IsiahM. Warner,
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摘要:
A rapid scanning fluorometer is used to obtain fluorescence spectral data characteristic of bacteria which do not readily produce fluorescent pigments. The data is generated in the form of an array of fluorescence intensity as a function of multiple excitation and emission wavelengths. Fluorescence spectral properties are introduced into the bacterial specimen using multiple dye mixtures for staining. The characteristic fluorescent fingerprints are believed to occur by preferential adsorption and reaction of bacterial cellular components with the individual dyes of the mixture. The procedure is found to be rapid and reproducible for the bacteria examined.
ISSN:0003-2719
DOI:10.1080/00032718108081450
出版商:Taylor & Francis Group
年代:1981
数据来源: Taylor
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8. |
Editorial board page for “Analytical Letters”, Volume 14, Number 13 |
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Analytical Letters,
Volume 14,
Issue 13,
1981,
Page -
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摘要:
This is a scanned image of the original Editorial Board page(s) for this issue.
ISSN:0003-2719
DOI:10.1080/00032718108081443
出版商:Taylor & Francis Group
年代:1981
数据来源: Taylor
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