摘要:

The synthesis of hyaluronic acid and proteoglycans by rat mucosal keratinocytes of an established cell line (CCL‐10) has been investigated. Proliferating cultures at or near confluency were grown in the presence of [35S] sulfate or D‐[1‐3H] glucosamine for 24 h, and the glycosaminoglycan composition of cells and medium was determined. Characterization of the35S‐laballedglycosaminoglycans showed that heparan sulfate was the major component (∼90%) and that small amounts (∼10%) of galactosaminoglycans had also been synthesized. Analysis of cultures labelled with D‐[1‐3H] glucosamine demonstrated that hyaluronic acid was also present, most prominently in the medium where approximately one third of the radioactivity in the glycosaminoglycan pool was found in the hyaluronic acid fraction. [35S]‐labelled proteoglycans extracted from the cell layer in the presence of protease inhibitors showed substantial heterogeneity upon chromatography on Sepharose CL‐6B. In contrast, the proteoglycans in the medium gave a major peak which was eluted at a Kavof 0.28. Gel chromatography of the glycosaminoglycan chains in the latter, isolated after proteolytic digestion, indicated a molecul

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01322.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The purpose of the present report was to document the stress response produced by physical and chemical abuses to human periodontal ligament cells, and to review some of the known functions of stress response proteins produced as a result of such treatments. For these studies human PDL cells were exposed to sublethal challenges of 43°C heat, sodium arsenite and the amino acid analog L‐azetidine‐2‐carboxylic acid (AZC). The cells were labelled with [35S]‐methionine and the proteins produced were examined by autofluorography of SDS‐PAGE gels. Heat challenges were shown to induce hsps with an apparent mol. wts. of 90K, 68‐72K, 41–47K, and 36 K. Arsenite‐treated cells produced similar hsps including a 30k protein not produced by other forms of stress. AZC treatment resulted in the production of apparent functionless hsps with apparent molecular weights of 90,000, 72,000, 68,000 and 36,000. The function of these proteins and their possible role in periodontal dise

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01323.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The maintenance of alveolar bone is a major clinical objective in dentistry. This is particularly difficult following such local inflammatory episodes as those of periodontitis or the loss of dentition (residual ridge resorption). We present evidence from beagle dogs that local infusion of prostaglandin E1(PGE) for 3 weeks at doses of 500 to 2000μg per week produces a dramatic, localized formation of alveolar bone in the mandible which exhibits a normal lamellar architecture and mineralization pattern when evaluated by fluorescence microscopy and microradiography. Whether this newly formed bone becomes functionally integrated into the skeleton and can replace bone lost from surgical resections or trauma remains to be established. Nevertheless, these data indicate that predictable local osteogenesis may eventually be produced by infusions of PGE

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01324.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Cell surface carbohydrates are excellent markers for cellular differentiation and maturation due to great structural and antigenic diversity and to known precursor/product relations. Several blood group related carbohydrate antigens were analyzed in human labial stratified non‐keratinized epithelium from 16 healthy individuals by immunohistology using monoclonal antibodies. The expression of these antigens was correlated with erythrocyte phenotype and saliva secretor status. Three distinct compartments of the epithelium were found and defined by the sequential expression of derivatives of Type 2 chain structures: lower, confined to basal cell layers (N‐acetyllactosamine), middle, to parabasal cell layers(H)and upper, to spinous cell layers (Ley/Lex). Although the antigens are related to blood group antigens they are largely expressed independently of the ABO, Lewis and secretor types, and may therefore serve as “universal” markers in differentiation studies of normal and pathological epi

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01325.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

The permeability of skin and oral mucosa to various compounds has been measured but the actual pathways substances take in traversing the epithelia have not been identified. In this study, radiolabelled cholesterol, ethanol or water were placed on the surface of porcine skin, keratinized gingiva, or nonkeratinized floor of mouth mucosa, and incubated at 37°C for 2 h. The tissue was then snap‐frozen, and sectioned in a cryostat, picked up on precoated slides and exposed at ‐20°C for 40 days for light microscopic autoradiography. Some tissues were freeze‐dried and directly embedded in low viscosity resin and prepared for electron microscopic autoradiography Examination of autoradiographs revealed silver grains over, or adjacent to, intercellular spaces. Counts of the grains over the extra‐ and intracellular compartments were made in random light and electron microscope fields. For all compounds and tissue regions, there were significantly more (p<0.05) grains over the intercellular spaces than over the cells. The results indicate that the intercellular compartment is the predominant route for compounds moving across the superficial barrier layer of epidermis and oral epithelia. The nature of the intercellular material is, thus, a primary determinant of epithelial per

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01326.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Cheek pouches of male Syrian golden hamsters were topically treated with a single dose of TPA (.5 μg), calcium ionophore A23187 (75 μgs) or Sn‐1,2‐dioctanoylglycerol (DiC8) (500 μg) dissolved in 0.25 ml acetone. Acetone‐treated animals served as controls. After 48 h the mitotic index for the control group was 1.1 ± 0.1 per 1 mm of the basement membrane length. All the test congeners exhibited higher mitotic indices than controls: TPA (4.8 ± 0.4), A23187 (3.9 ± 0.3), DiC8 (2.1 ± 0.2). All groups exhibited an increase in the epithelial thickness manifested by cellular hyperplasia. The treatment of the pouches with the anti‐inflammatory agent fluocinolone acetonide inhibited the milogenic and hyperplasiogenic affects on the epithelium induced by the various test chemicals. These studies indicate a possible role of calcium‐phospholipid dependent protein kinase (protein kinase C) in the mediation of oral epithelial ce

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01327.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

This study was undertaken to test the hypothesis that deregulated expression of the angiogenic phenotype by tumor cells is due to loss or inactivation of an angiogenesis suppressor gene(s). We used the technique of somatic cell hybridization to test the ability of untreated or chemical carcinogen‐initiated hamster pouch keratinocytes, when fused to squamous epithelial neoplasms, to suppress tumor angiogenic activity by assaying hybrid‐conditioned media (CM) in the avascular cornea of rat eyes. A non‐angiogenic keratinocyte line, CL‐2, derived from cultures of untreated epithelium and 3 lines of carcinogen‐initiated keratinocytes, PN3, 5, and 7, of varying angiogenic potential were fused, using polyethylene glycol, to 3 tumorigenic, potently angiogenic, drug‐resistant, hamster Serum‐free 48‐h CM from hybrid clones was prepared and assayed for angiogenic activity in rat corneas. CM from 5 hybrid clones derived from normal × neoplastic keratinocytes failed to induce an angiogenic response in 28 of 29 (97%) corneas tested. In contrast, CM from 4 hybrid clones derived from fusions between carcinogen‐initiated and tumor cells were potently angiogenic in 24 of 25 (96%) corneas tested. Two angiogenesis suppressed hybrids clones were propagated in culture for an extended period of time, to permit chromosome segregation, and were found to re‐express the angiogenic phenotype. These result indicate that angiogenesis is a recessive trait in normal hamster keratinocytes which is regulated intransin these hybrid cells. It would also appear that loss or inactivation of angiogenesis suppressor function occurs early in th

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01328.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Quantitative analysis of rat palatal mucosa after the carcinogen 4‐nitroquinoline‐1‐oxide had been applied to the epithelium for varying periods of time showed that there was a significant increase in epithelial thickness, due largely to an increase in thickness of the basal compartment. This alteration was measurable before epithelial dysplasia could be recog

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01329.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Complementary DNA (cDNA) clones corresponding to the 55 kDa (K 14) and 59 kDa (K 10) keratins were used as probes forin situhybridization analysis for the expression of keratin genes in human ameloblastomas and in oral mucosa. Transcripts for either the K 14 keratin or the K 10 keratin were restricted in their spatial distribution within stratified epithelia consistent with the stage of differentiation of the keratinocyte: the K 14 keratin gene transcript was restricted to the basal cell layers of the mucosa, while the K 10 kerattranscript was expressed predominantly in suprabasal cells, within the granular and prickle layers. In contrast, only the K 14 keratin transcript could be indentified within the epithelial cells of human ameloblastomas. The differentiation‐specific keratin transcript (K 10) was not present at detectable levels in this type of odontogenic tumors. In an atypical, infiltrating ameloblastoma, either the K 10 nor the K 14 transcript could be identified. Granular cells within one ameloblastoma expressed the K 14 transcript. A detailed examination of the pattern of gene expression in these unique tumors may lead to a better understanding of their pathogenesi

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01330.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

A clinicopathological study of 57 unicystic ameloblastomas has been undertaken, which represents 15% of all cases of ameloblastoma accessioned in our department over a 30‐yr period. Of the cases where gender was recorded: 30 were male and 23 female. The majority of patients were black (51 cases) and most occurred in the mandible (52). This distribution conforms to that of solid and multicystic ameloblastomas. The mean age at diagnosis was 23.8 years (S.D. 14.9) which is significantly younger than for the conventional counterpart (p<0.1%). The lesions were classified histologically into 3 groups: Group 1 (42%) cyst lined by a variable often non‐descript epithelium; Group 2 (9%) cyst showing intraluminal pelxiform proliferation of epithelium; Group 3 (49%) cyst with invasion of epithelium into the cyst wall in either follicular or plexiform patterns. While Group 1 and 2 lesions may be treated by enucleation, Group 3 lesions should be treated aggressively as for conventional ameloblastomas. The objectives of correct histological diagnosis, sub‐classification and appropriate therapy are best achieved by enucleation biopsy. There is little evidence to support origin from pre‐existing odontogeni

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01331.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY