摘要:

A number of changes occur in preneoplastic and neoplastic cells as they progress towards a greater degree of malignancy. These alterations include genetic changes, epigenetic changes, surface alterations and alterations in intercellular interactions. In some instances, these changes are contributing factors to the degree of pathology noted, whilst others are resultant. In many situations, the relationship between these changes and the progression towards neoplasia is not understood. Nevertheless, it seems probable that these changes are ultimately involved in driving cells further along the path to neoplastic transformation. It is the purpose of this review to consider the changes which occur as cells progress from normality to being neoplastic, with particular reference to the cells of the oral mucosa, and the use to which detectable changes can be used as prognostic indicators.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01534.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Early‐stage lesions of Kaposi's sarcoma (KS) are composed of single‐layered, highly flattened cells lining collagen bundles, whereas late‐stage lesions contain densely packed, spindle‐shaped cells. We examined the progression of KS lesions in oral mucosa and lymph nodes from patients with AIDS, using antibodies specific for blood vascular endothelial cells (Factor VHI‐related antigen) and their basement membrane (Type IV collagen and laminin). In addition, the plant lectinUlex europaeus, which selectively stains blood vessels, was also used. In early‐stage KS lesions, fibronectin, laminin and Type IV collagen were co‐distributed at the interface between KS cells and collagen bundles; Factor VHI‐related antigen andUlex europaeuslectin staining was present in vascular channels and in the KS cells. However, in late‐stage lesions, few if any KS cells stained with antibody to Factor VHI‐associated antigen, although endothelial cells lining blood vessels were positive. Strong staining for laminin and Type IV collagen was present in a pericellular pattern throughout the nodular late‐stage lesions. Since lymphatic capillary endothelium does not produce basement‐membrane‐specific macromolecules, these results support the conclusion that KS cells are related to blood vascular endothelium but eventually lose certain endothelium‐specific markers as the cells are transformed into t

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01535.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

A series of routinely processed, paraffin‐embedded biopsies from 73 surgically treated oral precancerous lesions (OPL) (22 cases), and oral squamous cell carcinomas (SCC) (51 cases), was first screened using an in situ DNA hybridization technique with a human papillomavirus (HPV) DNA probe cocktail containing the 35S‐labelled DNA of HPV types 6, 11, 13, 16, 18 and 30. The specific HPV types in lesions shown to contain HPV DNA in this procedure were further analysed by usingin situhybridization and the 6 HPV DNA probes separately. A total of 12/73 (16.4%) of the lesions proved to contain HPV DNA; 6/51 (11.8%) carcinomas and 6/21 (28.6%) dysplasias. The most frequent sites of HPV DNA‐positive lesions were palate (4/7; 57%), followed by the floor of the mouth (2/8; 25%), the tongue and gingiva (11.8%). HPV 13 or HPV 30 were not found in any of the lesions studied. HPV 11 DNA was demonstrated in 2 mild dysplasia lesions, but not in carcinomas. One additional mild dysplasia proved to contain HPV 6 DNA. HPV 16 DNA was present in 5 biopsies; 3 carcinomas and 2 dysplasias. In one of the HPV 16‐positive carcinomas, HPV 18 DNA was simultaneously present. HPV 18 alone was found in 3 additional carcinomas and in one moderate dysplasia lesion. The results confirm the recently reported evidence on HPV involvement in OPL and oral cancer. The implications of these findings are discussed in terms of the possible HPV etiology of oral SCC. The use of thein situDNA hybridization as a powerful tool (enabling the localization of specific HPV DNA sequences and the proper classification of the lesion at the same site) in the study of routinely processed oral biopsies is strongly ad

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01536.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Previous studies have shown that reduction of mastication has marked effects on the structure and biochemistry of the rat parotid gland. Acute starvation results in the formation in the acinar cells of large autophagic vacuoles which contain lysosomal hydrolases and within which secretory granules appear to undergo degradation. In this study we used electron microscopic immunocytochemistry and antibodies to two secretory proteins, oamylase and Brimmunoreactive protein, to determine whether secretory proteins are present in autophagic vacuoles of parotid acinar cells of starved rats. Small vacuoles were observed after 24‐h starvation; they increased in size and number up to 72‐h starvation. Both secretory proteins were present in the secretory granules and in the dense content of the autophagic vacuoles, as shown by immunogold labelling. The lighter matrix of the vacuoles was unlabelled. These findings confirm that secretory granules may fuse with lysosomal structures, where their content of secretory proteins is presumably degraded. Thus, the rat parotid appears to be similar to other secretory cells in which cellular levels of stored secretory proteins may be regulated by the process of crinoph

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01537.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

An immunoperoxidase staining technique was used for detecting alpha one‐antichymotrypsin (αl‐ACT), alpha one‐antitrypsin (αl‐AT), lactoferrin and transferrin in routine histological paraffin sections of 30 adenolymphomas, as well as in normal salivary gland tissue. Microscopically, the epithelial, component of adenolymphomas consisted of tall columnar luminal cells and irregularly shaped basal cells. αl‐ACT was detected in the luminal layer of epithelium in 27 (90%) of 30 adenolymphomas, while the basal layer was positive in 4 cases (13%). Lactoferrin could be observed in the columnar cells of 21 cases (70%) and was positive in the basal cells of 2 cases (7%). In normal salivary gland tissue, αl‐ACT and lactoferrin were observed in the intercalated duct and serous acinar cells. The staining pattern of αl‐AT in adenolymphoma was similar to those of αl‐ACT and lactoferrin, however, the number of positive cases for αl‐AT was fewer than in the αl‐ACT and lactoferrin. αl‐AT was not found in the normal salivary gland. On the contrary, the localization of transferrin in the epithelial component of adenolymphomas was exclusively different from those of αl‐ACT, αl‐AT and lactoferrin. Transferrin was found more often in the basal cells than in the tall columnar apical cells. The staining pattern of transferrin in the normal salivary gland was different from αl‐ACT and lactoferrin, and transferrin was positive in the cytoplasm of intercalated ducts, serous acinar and myoepithelial cells. Therefore, concomitant use of these 4 immunohistochemical stains may offer a way to study the functional condition of two‐layer epithelium of adenolymphoma. These results would indicate an intimate iinmunohistochemical expression between the normal intercalated duct and serous acinar cells and t

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01538.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

A flow cytometric analysis of peripheral blood lymphocytes was undertaken in recurrent aphthous stomatitis patients. The project aimed at detecting differences within lymphocyte subsets using type‐specific monoclonal antibodies. Peripheral blood samples were taken from RAS patients in both active and remission phases of the disease and from a group of healthy control subjects. There were no statistical differences between the active and remission phases within any of the lymphocyte subsets examined. There was, however, a significant difference between the RAS group and the control group. RAS patients have depressed CD4+ cell numbers and elevated CD8+ cell numbers. The CD4: CDS ratio is also depressed. A dissection of the CD4+ subset shows raised numbers of CD4+, 4B4+ lymphocytes and depressed numbers of CD4+, 2H4+ lymphocytes. Previous studies have shown disruption of peripheral blood lymphocyte numbers in Behcets syndrome. A similar pattern has now been shown in uncomplicated cases of minor RA

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01539.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

AbstractThe blood vessels in 3 cases of adenomatoid odontogenic tumour (AOT) were investigated ultrastructurally. An estimated 70–90% of the blood vessels found in the stroma showed degenerative changes which affected both the endothelial lining and the perivascular connective tissue. These vessels showed multiplication of basal lamina and were also encircled by concentric lamellae consisting either of collagen or fine filaments measuring 5–15 nm in diameter. Degradation of the layered collagen into fine filaments similar to those forming the concentric layers was observed. The present results suggest that the fine filaments of the concentric lamellae probably result from degradation of the layered collagen surrounding these vess

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01540.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

摘要:

Pediatric dentistry: infancy through adolescence. Pinkham JR

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01541.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1988.tb01542.x

出版商:Blackwell Publishing Ltd    

年代:1988

数据来源: WILEY