摘要:

Routinely formalin‐fixed and paraffin‐embedded material of 22 squamous cell carcinomas of the floor of the mouth (T2NoMo, Ro), together with adjacent dysplastic or normal mucosa, were immunohistochemically investigated using a panel of four anti‐p53 antibodies (CM1, PAbl801, DO7, PAb240) subsequent to wet autoclave pretreatment for antigen retrieval. p53 immunoreactivity was detected in 9/22 (40%) carcinomas with PAbl801 and DO7 antibodies, and in 8/22 cases using CM1 and PAb 240. p53‐positive tumour cells accumulated predominantly at the invasive front of the carcinomas. A focal or scattered p53 immunoreactivity was observed in the adjacent normal and/or dysplastic mucosa in 17/22 (77%) cases using both CM1 and PAbl801 antibodies, in 10/22 with DO7, and in 8/22 with PAb240. This study has demonstrated examples of different p53 immunophenotypes in the non‐tumorous and neoplastic oral mucosa of the same patient without significant correlation to the clinicopathological parameter

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01196.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The expression of extracellular matrix (ECM) proteins (fibronectin, laminin, collagen IV) and ECM receptors of integrin type (α2β1, collagen receptor; α6chain of the fibronectin receptor; α6chain of the laminin receptor) were examined in normal oral squamous epithelium and in invasive areas of oral squamous cell carcinomas with various differentiation and proliferation activities (Ki‐67 antigen labelling), evaluating the presence, quantity (using an image analysis system) and distribution of the integrin subunits. In the mucosa, there was uniform immunostaining for α2β1and α6concentrated at the cell membrane in the basal/supra basal cell zone, whereas, α5showed a discontinuous staining of the basal cell‐basement membrane interface. α2and α6could be visualized in all carcinomas α5showed low expression preferentally in less differentiated carcinomas. In contrast to normal mucosa, there was an increase in α6staining in well‐differentiated carcinomas. Dedifferentiation of oral carcinomas was accompanied by an increase in cellular proliferation and with a decrease in α2β1and α6staining. This reduction of α6staining was shown to be statistically significant, suggesting that this integrin may be a valuable grading parameter for oral sq

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01197.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The purpose of the investigation was to evaluate and compare the proliferation (growth) of mouth fibroblasts and skin fibroblasts from patients with oral submucous fibrosis (OSF). Material comprised fibroblasts from fibrous bands situated in the buccal mucosa and from the inner aspect of the forearm of 8 patients with classic features of OSF as well as fibroblasts from 6 buccal mucosa and 8 skin biopsy specimens from healthy non‐areca nut chewing individuals. Cells were cultured for 8 days according to standard techniques. Their growth was monitored daily, under optimal conditions as well as exposure to concentrations of arecoline. The data were analyzed using regression analysis, analysis of variance and the Kruskal‐Wallis test. We found no statistically significant differences between the proliferation patterns of oral and skin fibroblasts from patients or between those from patients and controls. The reaction of the cells exposed to concentrations of arecoline was similar; at low concentrations (0.1–10 μg/ml) normal growth was maintained, while 100 μg/ml inhibited growth. It is concluded that fibroblasts from mouths affected by OSF have proliferation patterns which fall within normal parameters, that the excessive collagen formation in established OSF is not due to increased fibroblast proliferation and that arecoline does not stimulate fibroblast prolif

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01198.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

We have investigated the correlation between the prevalence of hepatitis C virus (HCV), which is detectable in saliva, and oral cancer and other digestive tract cancers in the Northern Kyushu region of Japan. Anti‐HCV antibodies were detected in sera from 24 of the 100 patients with oral cancer (24%, p<0.05 vs the control group, p<0.01 vs the stomach cancer group), in 11 of 104 patients with non‐malignant diseases receiving dental treatment (the control group, 10.6%), and in 12 of 113 patients with stomach cancer (10.6%). HCV‐RNA was detected in sera from 17 of 100 oral cancer patients (17%, p<0.05 vs the control group) and 4 of 104 patients of the control group (3.9%). These results indicate a high prevalence of HCV infection in oral cancer patients, which warrants a systematic study of etiological associations between oral cancer an

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01199.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Asymptomatic oral candidal carriage has been extensively studied in adults, but only rarely in infants. The present investigation aimed to determine the asymptomatic oral carriage of candidal species in healthy infants and its relationship to age, sex, feeding pattern and use of a pacifier. The swab technique was used for oral candidal isolation. Candidal species were isolated from 48% of the infants without a significant relationship to age, gender or between breast‐fed or bottle‐fed infants. Similarly, a history of maternal vaginal candidosis, or presence of nappy rash, had no significant relationship to oral candidal carriage. Infants who routinely sucked a pacifier had a significantly higher rate of oral candidal carriage, suggesting a reservoir of infect

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01200.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

A functioning dental follicle and bone resorption are necessary for tooth eruption. In the microphthalmic (mi) mouse, bone resorption is defective and teeth fail to erupt. Local bone metabolism involves the production of cytokines such as interleukin‐1 alpha (IL‐1α) and transforming growth factor‐β (TGF‐β). The production of these cytokines by dental tissues during eruption may be fundamental to tooth movement. Molars frommimice were cultured and supernatants tested for the presence of these cytokines using bio‐dot blotting and ELISA. A thymocyte bioassay was used to test supernatants for IL‐1 bioactivity and IL‐1 inhibition bioactivity. IL‐1α and TGF‐β were detected in all supernatants. Supernatants demonstrated no IL‐1 bioactivity but inhibited IL‐1 bioactivity which varied with concentration of supernatant, age and animal. This study demonstrated that cultured developing teeth secrete IL‐1α and TGF‐β however, concentrations varied in normal and pathological states. While IL‐1α was present in the supernatants, all demonstrated a variable ability to inhibit IL‐1 bioactivity. This ability may influence local bon

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01201.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Two cases of adult linear IgA disease (LAD) with oral and colonic involvement are presented. Oral findings, for both patients, were of widespread painful ulceration present for two to three months and diagnosis was confirmed by biopsy and direct immunofluorescence. Colonic symptoms preceded oral symptoms in both cases and a number of investigations were undertaken, including routine histological examination, which suggested either lymphocytic colitis or Crohn's disease. Immunofluorescence studies showed linear IgA deposition confined to the distal colon. These cases highlight that large bowel involvement can be a feature of linear IgA disease and for the first time demonstrate IgA deposition at that site.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01202.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

We describe a vascular tumor classified as SCH by histological criteria that was found in the mandibular‐buccal fold of a 12‐year‐old girl. Microscopically, the lesion consisted of thin‐walled cavernous spaces containing thrombi and phleboliths, and cellular areas composed of spindle‐shaped, epithelioid and vacuolated cells. Immunohistochemically, the endothelial vascular lining was highly reactive with HAM56 antibody, while variable reactivity was observed for factor VIII‐associated antigen. All cell types were positive for vimentin and anti‐PCNA stained less than 3% of the tumor cells. This is the first report of SCH in th

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01203.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

A 9‐year old Black boy with gigantism of the hands and feet, and recurrent gingival hyperplasia, diagnosed as Proteus syndrome is presented. The oral manifestations of this syndrome are described. To the best of our knowledge, this is the first reported case of gingival hyperplasia associated with Proteus syndrom

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01204.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY