摘要:

Oral lichen planus (LP) is a common mucosal disorder in which cell mediated immunity is thought to play a major role. In this paper, a unifying hypothesis which attempts to integrate cellular and molecular signals in the local immune response in oral LP is presented. In this model, modified keratinocyte surface antigens are the target for the cytotoxic cell response which characterizes oral LP, whereas mast cells and antigen presenting Langerhans cells are key cellular elements in the evolving lesion. It has been established that mast cell degranulation induces adhesion molecule expression on endothelium which facilitates lymphocyte homing to the tissues. These adhesive interactions between lymphocytes and keratinocytes are postulated to be important determinants in the effector phase of the lesion. Cytokines produced by both lymphocytes and keratinocytes which influence the local immune response could promote chronicity. Accordingly, modulation of immunologic events is a potential therapeutic approach for oral LP.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1990.tb00866.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Cyclosporin A (CSA)‐induced gingival overgrowth was immunohistochemistry compared with that phenytoininduced and nonspecific inflammatory gingiva, and CSA concentration was determined for dental plaque. Leu‐6+epithelial dendric cells (EDC) were found to significantly decrease in number in CSA‐induced gingival overgrowth, while the ratio of HLA‐DR+ EDC to Leu‐6+EDC did not change significantly. The expression of class II major histocompatibility complex antigens, such as HLA‐DR, ‐DP and ‐DQ on keratinocytes did not change by CSA‐treatment. Leu‐4+mononuclear cells in CSA‐induced gingival overgrowth were located primarily in the connective tissue far outside the epithelium. CSA concentration was much higher in dental plaque than in blood and other tissues. Immune response thus appears to be suppressed in the epithelial layer of CSA‐induced gingival overgrowth through decrease in Leu‐6+HLA‐DR+EDC and T cell infiltration, both due to CSA in dental plaque. DNA polymerase a was detected in much fewer basal keratinocytes of CSA‐ and phenytoininduced gingival overgrowth. Epithelial hyperplasia may thus be not due to increased keratinocyte proliferation, but rather to en

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1990.tb00867.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Phenytoin (PHT), a widely used anticonvulsant, and nifedipine (NF), an antianginal drug, cause clinically similar gingival overgrowths in some patients. The aim of this work was to investigate their effects on collagen and protein synthesis and cellular proliferation in normal human gingival fibroblasts in vitro. Gingival fibroblasts were cultured from biopsies taken from three healthy individuals during operations on maxillary canines and incubated with various concentrations of NF (100 and 200 ng/ml) and PHT (5 and 10 ng/ml) for up to 7 days. The results showed that NF and PHT have a specific effect in reducing total protein and collagen synthesis but do not influence cell proliferation in healthy gingival fibroblasts in vitro. In addition the level of mRNA for type I collagen was decreased after incubation of the cells with the drugs for 1 or 2 days. The decrease in the level of type I collagen mRNA seemed to be specific since the level of type IV collagenase mRNA used as a reference RNA did not decrease.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1990.tb00868.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Normal tongue and cervical mucosa, premalignant cervical and vulvar lesions, primaries and metastases of squamous cell carcinomas from the oral, laryngeal, cervical and vulvar mucosa were analyzed for c‐erbB2 and c‐myc transcription with northern‐blots using 32P single‐stranded RNA probes. Transcription of c‐erbB2 and c‐myc could be detected for almost all tissues including normal samples. A slightly enhanced transcription level was found in three cervical intraepithelial neoplasias of Grade IIT (CIN III) but in none of the malignant lesions. Increased transcription of c‐myc was observed in premalignancies and malignancies. It was more frequent in oral and laryngeal squamous cell carcinomas (SCC) (8 of 9 cases) than in genital SCC (3 of 11 cases) or premalignancies (3 CIS of 14 CIN/VIN). No relationships of c‐myc enhanced transcription level with tumor grading and staging were noticed. Thus, mere oncogene expression is a widespread phenomenon in tissues of different histogenesis and quantitative analysis is necessary prior to ascribe any diagnostic or prognostic relevance. Moreover, the frequency of tumors with enhanced transcription may vary for phenotypically closely related tumors of d

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1990.tb00869.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

We assessed the ability of monoclonal antibody (MAb) 17.13 to react with human oral squamous cell carcinoma (OSCC) and other oral tissues. MAb 17.13 was reacted with acetone‐fixed, serial frozen sections of 21 Oscc, 7 fibromas, 2 squamous papillomas, 1 melanosis and 3 normal oral mucosa, by means of an avidin‐biotin‐immunoperoxidase assay. Twenty‐one of 21 tumors (100% sensitivity) reacted strongly with MAb 17.13, with a homogenous staining pattern. Individual tumor cells could be clearly seen, improving the detection of microinvasion or borderline lesions. The epithelium of benign lesions and normal oral mucosa showed staining of the basal cells only. We conclude MAb 17.13 could be used as an accurate diagnostic tool f

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1990.tb00870.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Intravascular papillary endothelial hyperplasia (IPEH) is usually a cutaneous lesion thought to represent a peculiar manifestation of an organizing thrombus. IPEH can be subdivided into a pure form that occurs within a dilated vascular space and a mixed form that appears as a focal change within a preexisting vascular lesion, such as hemangioma. The principle significance of the lesion lies in the fact that it may be mistaken for angiosarcoma. Oral lesions are uncommon and the present paper reports on 16 new cases of oral IPEH, analyzing their clinical and histomorphologic features. Histologically, 11 lesions appeared in a pure form and 5 lesions in a mixed form. Thrombi were detected in all but one case. The most common location was the lip, followed in decreasing frequency by the tongue and buccal mucosa. Local excision was the treatment of choice.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1990.tb00871.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

The masseter and temporalis muscles were studied ultrastructurally in eight cases suffering from restricted mouth opening and TMJ ankylosis. The changes varied from slight degeneration of muscle fibers, such as accumulation of lipid or lipofuscin deposits, Z‐band streaming and derangement of myofilaments, in one case to moderate or severe changes of muscle atrophy and necrosis in the remaining seven cases. Nemaline bodies were also observed in one of these seven cases. In addition to these muscle changes neural degeneration especially of myelinated nerves were found in three of the cases studied. The present findings showed that in spite of the diversity of the underlying factors affecting the joint, there was many similarities in the changes observed within the muscle. It is postulated that these degenerative muscle changes probably play an important role in restricted mouth opening and TMJ ankyiosis, and that some of these muscle pathology may be the result of neural involvemen

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1990.tb00872.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

Young albino mice, 4‐days‐old, exposed to 0.1% nicotine sulfate on gestational days 6–20 were compared with untreated pups to determine nicotine's effects on the animals. Pregnant mice were given intraperitoneal injections of nicotine at a dose of 1.67 mg/kg/day. Data of pups weight, CRL, distance between the anterior and posterior limbs, maximum head height and width as well as head circumference were collected. The neonates were then decapitated, their entire mandibles were excised, routinely prepared, sectioned and stained with H and E for histologic examination of the developing incisors. All the parameters were significantly reduced in the treated group. Histologically, the processes of odontogenesis appeared retarded in nicotine treated animals, whereas the control group demonstrated normal dentin and enamel formation. Thus nicotine has a detrimental effect on the development of newborn offspring in general and incisor development in particular. This effect begins prenatally and extends postna

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1990.tb00873.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

A variant of calcifying epithelial odontogenic tumor (CEOT) with Langerhans cells is reported. Compared to a typical CEOT, the tumor islands of this case were thin and composed of a small number of polyhedral epithelial cells. Almost no calcification of homogenous eosinophilic materials was observed. In addition, clear cells which structurally corresponded to Langerhans cell were intermingled in the epithelial islands. These cells stain positively for S‐100 protein, lysozome, MT 1, LN‐3 and OKT 6 antibodies, but not for keratin antibody. Electronmicroscopic examination revealed the rod‐shaped and racket‐shaped structures called Birbeck's granules in the cytoplasm of these clear cells. Our observations indicate a variant case of CEOT with Langerhans cells in tumo

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1990.tb00874.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY

摘要:

This study in scanning electron microscopy (SEM) was carried out to observe the possible pathologic aspect of five teeth grossly arranged in normal sequence and of their surrounding tissues, found inside an ovarian cystic teratoma. Though the general morphology of the teeth was nearly normal, several anomalies affected the different mineralized dental tissues such as enamel hypoplasia, irregular growth of cementum, altered predentin layer, and immature osteofibrous bony outgrowths.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1990.tb00875.x

出版商:Blackwell Publishing Ltd    

年代:1990

数据来源: WILEY