ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01205.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Fibroblast growth factor‐1 (FGF‐1) and FGF‐2 are mitogenic polypeptides that may contribute to neoplastic cell proliferation. In the present study, we established a serum‐free culture system for ameloblastoma cells and demonstrated that the addition of FGF‐1 and FGF‐2 enhanced cell growth in a dose‐dependent manner. Immunoperoxidase staining of cultured cells demonstrated strong expression of FGF‐1 and FGF‐2. In tissue specimens, FGF‐1 was localized in epithelial cell components of ameloblastomas, whereas FGF‐2 was mainly found in the basement membranes with only moderate staining in epithelium. These data suggest that both FGF‐1 and FGF‐2 may contribute to the growth and deve

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01206.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Forty‐five examples of epithelial odontogenic lesions (9 ameloblastomas (AB): 13 odontogenic keratocysts (OKC): 15 dentigerous cysts (DC): 6 radicular cysts (RC): and 2 odontogenic carcinomas (OC)) were immunohistochemically analyzed for the presence of p53 protein (p53P) and proliferative activity as indicated by positivity for Ki‐67 antigen. p53P+cells, detected as dense and/or faint nuclear staining, were found in 42 of the 45 odontogenic lesions examined. Dense p53P reactivity was most commonly detected in OKC, AB and OC, with other lesions generally exhibiting only weak nuclear reactivity. Numbers of Ki‐67 positive cells as well as p53P+cells were scored semiquantitatively. Although the presence/absence of densely stained p53P+cells was broadly related to Ki‐67+cell numbers, there were no differences in p53P+cell numbers between lesions exhibiting differences in proliferative activity. These results suggest that overexpression of p53P, rather than increased numbers of p53P+cells, is related to proliferation in odontogenic epithelial

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01207.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Aberrations of chromosomes 3 and 17 were studied by FISH using centromere‐specific DNA probes in 11 salivary adenoid cystic carcinomas (ACC) and 8 salivary pleomorphic adenomas (PA), with 3 lymph nodes as controls. Two hybridized signals were detected in 92.8±2.7% of controls, 73.2±7.0% of PA and 66.8±7.9% of ACC cells for chromosome 3, and in 90.4±2.3% of controls, 59.5±25.0% of PA and 44.8±20.2% of ACC for chromosome 17. More than 3 hybridized signals, which indicate polysomy, were observed in 3.1% of controls, 15.5% of PA and 22.9% of ACC cells for chromosome 3, and in 1.2% of controls, 10.3% of PA and 23.1% of ACC cells for chromosome 17. A single hybridized signal was much more frequent for chromosome 17 than for chromosome 3. These findings suggest that polysomy of both chromosomes occurs during the development of salivary gland tumors, and its frequency is increased in adenoid cystic carcinoma as compared to pleomorphic adenoma. In addition, monosomy of chromosome 17 could possibly be significant in salivary gland

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01208.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Over a 10‐year period (1982–1991), a total of 150 patients divided into two groups with varying degrees of oral submucous fibrosis (OSF) were treated by either medical or surgical therapies. Medical treatment involved (a) conservative oral administration of vitamin B‐complex, buflomedial hydrochloride and topical triamcinolone acetonide 0.1%, or (b) conventional submucosal injections of a combination of dexamethasone and hyaluronidase, or (c) a combination of both (a) and (b). The surgical group was treated by the excision of fibrotic tissues and covering the defect with split‐thickness skin, fresh human amnion, or buccal fat pad (BFP) grafts. Treatment was chosen according to the stage of clinical progression to gain maximal interincisal distance (ID). The cases were followed up by monthly examinations for at least two years, or when possible even longer. A combination of (a) and (b) medical treatment was satisfactory in cases of mild impairment (ID>20 mm) but in the long term it led to symptomatic relief only. Surgical therapy, on the other hand, when accepted by the patients, led to a significant improvement of trismus in cases of severe limitation (ID<20 mm). Following this strategy, an additional ID increase was observed in all patients. BFP grafting was particularly successful in diminishing scarring after two years as compared with the other two grafts. Together with a cessation of the betel quid chewing habit before and after therapy, these treatment regimens combined with daily mouth opening exercises were found to be necessary to manage OSF cases in early and advanced stages of prog

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01209.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Growth characteristics and lysyl oxidase activity of fibroblasts derived from human normal mucosa (NM) and oral submucous fibrosis (OSF) associated with betel nut chewing were compared in cell cultures. The growth rates of cultured cells were identified by plating 5×105 cells/35 mm culture dish (Day 0) and every 24 hours cell proliferation was determined by quantifying the cell number (using a hemocytometer). The third to seventh passages were used. A medium without serum but supplemented with 5 mg/ml bovine serum albumin was substituted for the original medium at the subconfluent period and cultured for an additional 24 h. The medium was collected and used for assays of protein content and lysyl oxidase activity. Lysyl oxidase activity was assayed with [4,5‐3H] ‐lysine labelled purified chick ‐ embryo aorta elastin substrate. After incubation for 10 h at 37°C, the enzyme activity was measured from 3HHO (tritiated water) separated by ultrafiltration using Amicon C‐10 micro‐concentrators. The results showed the mean doubling time of OSF fibroblasts was 3.2 days and of NM fibroblasts was 3.6 days. NM fibroblasts became confluent at day 6 as determined by cell number, while OSF fibroblasts were confluent by Day 5. Furthermore, the immunoenzymatic assay for BrdUrd incorporation revealed that OSF fibroblasts proliferate significantly faster than NM fibroblasts under standard culture conditions. Both total protein content (10.84±1.15 mg/ml) and lysyl oxidase activity (3558.6±345.5 cpm/106 cell) in OSF fibroblasts were greater than in NM fibroblasts (6.35±0.96 mg/ml and 2436.0±352.6 cpm/106cell). The results of this study provide evidence that fibroblasts derived from oral submucous fibrosis (OSF) tissue and normal mucosa (NM), although similar in many respects, exhibit specific differences in proliferation rates and lysyl oxidase activity. Moreover, collagen deposition in OSF tissue may, at least in part, be ascribed to increased lysyl o

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01210.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

A quantitative, immunohistologic evaluation of CD3+, CD4+and CD8+cells was carried out on gingival biopsies from 25 HIV‐infected persons with gingivitis or periodontitis and 13 HIV‐seronegative persons with periodontitis. CD3+T cells were found in all biopsies. CD8+cells were significantly more numerous and the CD4+/CD8+ratio was significantly decreased in the gingival connective tissue of the HIV+patients (P<0.05). The number of CD4+lymphocytes subjacent to the pocket epithelium was moderately lower in the HIVH patients as compared to the HIV+patients (P<0.05). HIV+patients with a history of necrotizing periodontal disease had fewer CD4+cells subjacent to the oral gingival epithelium than patients without such disease (P<0.05). The general HIV‐related changes in T lymphocyte numbers were therefore reflected in inflamed gingival tissues. HIV+patients had, however, significantly higher CD4+/CD8+ratios in gingiva than in peripheral blood (P<0.05), indicating that CD4+T cells are actively recruited to gingiva, even in cases of extreme CD4+T lymphocyto

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01211.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The effects of recombinant human interleukin‐1β (rhIL‐1β) on alveolar bone resorptive activity in rats were examined. Continuous administration of rhIL‐1β or phosphate‐buffered saline (PBS) was given via osmotic pumps for 3, 7 and 14 days to rats with silk ligatures around second maxillary molars. Other animals without ligatures received insertion of pumps containing rhIL‐lp or remained untreated. Sections were subject to three different stains:–hematoxylin and eosin (H‐E) for histology, acid phosphatase (ACPase) activity for osteoclast detection, and immunohistochemistry using anti‐rat monocyte/macrophage monoclonal antibody (ED 1). In addition, body weight, plasma calcium and phosphorus levels were monitored. The mean body weight of rats receiving rhIL‐lp was significantly lower (P<0.05 toP<0.01) compared with untreated rats throughout the experimental period. On Day 7, plasma calcium and phosphorus levels were significantly lower in rats receiving rhIL‐1β than in rats receiving PBS only (P<0.05). Sections revealed a moderate inflammatory cell infiltrate reaching near the alveolar crest in both groups with ligatures on Day 3. Only rats receiving rhIL‐lp exhibited enhancement of inflammatory cell invasion on Days 7 and 14. In rats receiving rhIL‐lp with ligatures, numerous resorption lacunae containing ACPase‐positive multinucleated giant cells (MNGCs), coinciding with ED1‐positive cells, were located on the mesial side of the septum where extensive bone resorption had occurred throughout the experimental period. In animals receiving rhIL‐β without ligatures, compared with untreated rats, increased ACPase‐positive cells were observed on the mesial side of the septum on Day 3. In animals receiving PBS only, a few ACPase‐positive cells were observed confined to the mesial regions where slight bone resorption occurred on Days 7 and 14. These results indicate that the administration of rhIL‐1β accelerated alveolar bone destruction in ligature‐induced periodontal

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01212.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01213.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY