摘要:

This study investigated 84 patients with burning mouth syndrome (BMS), who were asked to complete a hospital anxiety and depression (HAD) scale questionnaire. A control group of 69 patients was also included. All patients were interviewed regarding parafunctional habits and were subjectively examined for signs of occlusal wear of the natural teeth or dentures. The results demonstrated that parafunctional habits were present in 61% of patients with BMS. There was a statistically significantly relationship between parafunctional habits and anxiety as indicated by the HAD scale, but not with depression.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01186.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

To investigate the role of herpes viruses in Sjogren's syndrome, minor (labial) salivary gland tissues from Sjogren's syndrome and from non‐specific sialadenitis were examined for Epstein‐Barr virus (EBV) and human cytomegalovirus (HCMV) DNA by the polymerase chain reaction. Almost half of all salivary glands studied contained EBV and/or HCMV. There was, however, no significant difference between the detection of EBV or HCMV in salivary glands from patients with Sjogren's syndrome or non‐specific sialadenitis. The findings are consistent with the persistence of EBV and HCMV in minor salivary glands following primary infection, but do not indicate a direct role for either virus in the aetiology of Sjogren's syndrome, and do not exclude reactivation of the viruses in this di

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01187.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

We examined the content of type IV collagenases and plasminogen activators (PAs) in luminal fluid of four extravasation mucoceles by means of gelatin or casein zymography. Immunohistochemical staining for type IV collagenases and PAs was performed to identify the possible source of these enzymes. The luminal fluid examined by gelatin zymography showed a high level of type IV collagenases compared with saliva from Wharton's duct. PAs were detected in only one of the cases by casein zymography, and none was detected in the saliva. By immunohistochemical staining, type IV collagenases and PAs were detected in duct and myoepithelial cells of the adjacent salivary gland and in macrophages and fibro‐blasts of the cyst wall. The results suggest that proteolytic enzymes are involved in the pathogenesis of mucocele

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01188.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

To determine whether testosterone administration was capable of modifying salivary gland carcinogenesis, female mice were given 1 mg of 9,10‐dimethyl‐1,2‐benzanthracene (DMBA) into the left submandibular gland and then Group 1 mice received 5 mg of testosterone propionate and Group 2 mice received vehicle, olive oil, subcutaneously for 8 weeks. Twelve weeks after the start of the experiment, the weight of the left submandibular gland of the Group 2 mice was greater than that of the Group 1 mice. The incidences of submandibular gland carcinoma in Groups 1 and 2 were 41% (12/29) and 57% (17/30), respectively. Epidermal growth factor (EGF) levels of the left submandibular gland were significantly higher in Group 1 as compared with Group 2. These findings indicate that testosterone increases the production of EGF in the DMBA‐injected submandibular gland, but does not promote the development of submandibular gland ca

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01189.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The purpose of this study was to determine whether a consistent association exists between mucous cells and clear or vacuolated cells in the epithelial lining of radicular and residual cysts and to consider whether the vacuolated cells may represent a stage in the histogenesis of mucous metaplasia in these linings. Single sections from each of 154 mandibular radicular and residual cysts were stained with periodic acid‐Schiff (PAS) after diastase digestion. Fifteen cases which showed mucous metaplasia were included in the study and were examined for the presence of vacuolated cells associated with the mucous cells. Mucous cells were present singly or in groups within all layers of the stratified squamous epithelial lining except the basal cell layer. In nearly all instances small to large ovoid vacuolated cells were found closely associated with the mucous cells. Occasional vacuolated cells contained sparse mucin granules or a delicate network of PAS‐positive, diastase‐resistant material. It is suggested that the clear cells may represent a stage in the histogenesis of mucous metap

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01190.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Proliferating cell nuclear antigen (PCNA) is a nuclear protein synthesized in the late G1and S phase of the cell cycle which can be detected immunohistochemically in paraffin‐embedded tissue as a useful marker for the proliferating fraction of cells in tissue specimens. Thirty‐eight cases of oral squamous cell carcinoma were studied in groups according to clinical staging and histological grading (UICC system), these included 5 cases of T1, 13 cases of T2, and 20 cases of T3, and 19 cases of G1, 12 cases of G2, and 7 cases of G3. The mean percentage of PCNA positively stained tumor cells in this series was 29.2. The mean percentages of PCNA‐positive cells in each stage and grade were: T1 (13.7%), T2 (24.4%), and T3 (34.3%); G1 (30.5%), G2 (248%), and G3 (33.2%). The results indicate that the percentage of PCNA positively stained tumor cells in oral squamous cell carcinoma was significantly greater when tumor size was larger than 2 cm (T1 versus T2/T3). However, there was no significant difference between T2 and T3. There was no correlation between the number of PCNA‐stained tumor cells and histologica

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01191.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The expression and localization of glutathione S‐transferase (GST) isoenzymes in the epithelium of normal oral mucosa (n= 9), overlying reactive fibrous hyperplasia (n=9), and of potentially malignant [leukoplakia (n=25), submucous fibrosis (n=12), verrucous hyperplasia (n=16)] and malignant [squamous cell carcinoma (n=36), verrucous carcinoma (n=13)] oral lesions were examined immunohistochemically using polyclonal antibodies raised against GST isoenzymes (alpha, mu and pi) with the standard avidin‐biotin‐peroxidase complex (ABC) method. GST alpha, mu and pi were almost completely absent in the epithelium of normal oral mucosa and overlying benign fibrous tissues. GST alpha staining was cytoplasmic and focally positive, while GST mu staining was similar to but weaker than that seen for GST alpha. GST pi showed both cytoplasmic and nuclear staining and was expressed in 60% of leukoplakias with mild dysplasia (n=15), 80% of leukoplakias with moderate to severe dysplasia (n=10). 75% of submucous fibrosis samples (n=12), 75% of verrucous hyperplasias (n=16), 77% of verrucous carcinomas (n=13), 81% of well‐differentiated squamous cell carcinomas (n= 26) and 70% of moderate‐ to poorly‐differentiated squamous cell carcinomas (n=10). In addition, GST pi expression was independent of the state of differentiation of oral cancers. Since GST pi was significantly over‐expressed in the oral premalignant and malignant lesions, the kinetics of GST pi‐positive cells and the value of GST pi as a tumor marker in oral carcinogenesis need furthe

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01192.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Intracellular levels of ornithine decarboxylase (ODC) are raised following mitogenic stimulus and in neoplasia. Because lesions of the oral cavity are often difficult to assess histologically, we have determined the value of immunocytochemical detection of ODC as a prognostic indicator in 74 routinely fixed and paraffin‐embedded oral biopsies using peroxidase‐antiperoxidase and immunogold‐silver staining. The latter proved more sensitive, yielding positive reactions in 32 of 43 oral carcinomas (11/14 well differentiated, 16/21 moderately differentiated and 3/5 poorly differentiated) and 7/11 potentially malignant lesions, compared with 19/45 carcinomas and 1/15 potentially malignant lesions, by peroxidase anti‐peroxidase. Hyperplastic lesions (n= 7) and normal non‐keratinized buccal mucosa (n= 7) were all negative. Follow‐up was possible in 13 of the carcinoma patients. Of 7 positive ODC reactions but clinically node‐free at biopsy, 2 died and 2 had recurrences within 3 years, whereas all of 6 with no immunoreactivity were symptom‐free after 3–5 years. Immunostaining for ODC may be helpful for the prognostic assessment of routinely processed oral lesions and in c

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01193.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The polymerase chain reaction (PCR) and direct DNA sequencing have been used to identify strain variants of HPV types 2a/57 in formalin‐fixed sections of human oral verrucae, where the virus had previously been detected by both immunofluorescence andin situhybridization. By employing type‐specific and type‐common PCR primers we show that these lesions contain a mixture of viral DNAs which vary by up to 27% in DNA sequence, in a region where the variation between HPV types 2a and 57 is only 4%. The extra discriminatory power of fluorescent sequencing indicates that the lesions may also contain wild‐type HPV2a/57 DNA which could provide a helper function for defective viral DNA molecules or indicate a mosaic origin for the

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01194.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01195.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY