摘要:

Proliferative verrucous leukoplakia (PVL) is a recently described clinical entity characterized by multifocal oral lesions that frequently progress to oral cancer despite abstinence from tobacco use by most patients. To determine if this condition is associated with human papillomavirus (HPV), a polymerase chain reaction (PCR) for HPV DNA was performed on 9 lesions from 7 patients with PVL, histologically diagnosed with focal keratosis (1), papilloma (1), epithelial dysplasia (5) and squamous cell cancer (2). Eight (89%) were HPV positive, 7 for HPV 16. For comparison, we studied 55 non‐PVL‐associated oral specimens, including 24 oral squamous cell cancers. Of the cancers, 8 (33%) were HPV positive, 4 for HPV 16. These data suggest that HPV 16 infection may play an important role in the pathogenesis of PVL‐associated oral dysplasia and possibly cancer, but is found in only a small proportion of the more common, non‐PVL associated‐ora

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01165.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Histopathologic examination of oral leukoplakias has a major impact on the assessment of prognosis and treatment planning. We investigated the extent of agreement in grading epithelial dysplasia between pathologists with the same or different educational backgrounds. Two general pathologists and two oral pathologists were each given 100 sections of oral leukoplakia to grade from no dysplasia to carcinomain‐situ.The interobserver agreement rates were in the range of 49% to 69%. The calculated kappa values were in the range of 27% to 45%), showing poor to moderate agreement between the pathologists. When comparing the kappa values between the two pairs of pathologists with the same education, these values did not diverge from the general level of kappa values, indicating that the interobserver variability was due to individual differences rather than to educational backgroun

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01166.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

An immunohistochemical study of p53 protein was carried out on 45 salivary gland lesions using a monoclonal antibody, Bp53–12, raised to the intracellular domain of the p53 protein. p53 protein expression was found in 34.4% of 32 salivary gland carcinomas. Nuclear p53 expression was detected in tumor cells but not in non‐neoplastic cells, except in one salivary duct carcinoma. The perinuclear cytoplasm of luminal duct cells was specifically positive for the antibody used here. Cytoplasmic p53 expression was observed mostly in non‐neoplastic cells. There was a tendency for the Cytoplasmic staining of p53 protein to be observed in the normal cells adjacent to p53‐positive carcinomas, but none of the normal cells were positive in the tissues surrounding p53‐negative carcinomas. Cytoplasmic expression of p53 protein in salivary gland tissues seems to be correlated with tumo

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01167.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Earlier studies have described an antibody that recognized salivary ductal epithelium in sera from 15–50% of patients with primary Sjögren's syndrome; however, the specific salivary antigen in those studies was not identified. The present study further investigated this unknown salivary antigen. Twenty‐nine of 31 patients (94%) with primary Sjögren's syndrome demonstrated IgG antinuclear antibodies that bound to an epithelial cell line with ductal characteristics derived from a human salivary gland. Seventy‐seven percent of these patients had serum antibodies that bound to ductal cells of normal human parotid tissue after formalin fixation. Western blots of cell extracts, immunofluorescence, and adsorption studies indicated that SS‐A/Ro and SS‐B/La were the antigens recognized in the salivary cell line. The pattern of fluorescence seen when anti‐SS‐B/La bound to normal parotid tissue was identical to the fluorescence pattern of the anti‐salivary ductal antibodies described in e

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01168.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The effect of cognitive therapy (CT) on resistant burning mouth syndrome (BMS) was studied. Thirty patients with resistant BMS after odontological and medical treatment were randomly divided into two equal groups; a therapy group (TG) was treated with CT and an attention/placebo group (APG) served as a control group. The intensity of BMS, which was estimated by the use of a visual analogue scale, was significantly reduced in the TG directly after CT was completed and was further reduced in a 6–month follow‐up. The APG did not show any decrease in intensity of BMS. The results of this study indicate that, in some cases, resistant BMS probably is of psychological ori

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01169.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

In an attempt to verify the nature of bone resorption processes on the bone surrounding radicular cysts, fragments of cysts with the adjacent bone tissue were studied by morphological, cytochemical and ultrastructural methods. Cyst fluid was analysed for its content of cytokines with osteolytic activity. The cyst wall exhibited several connective tissue extensions which penetrated the adjacent bone. Numerous multinucleated tartrate‐resistant acid phosphatase (TRAP)‐positive cells were seen at the tip of the intraosseous extensions of the cyst capsule and in direct contact with the bone tissue. Typical resorption lacunae were identified on the bone surface by scanning electron microscopy. Moreover, mononuclear TRAP‐positive cells were seen within the cyst capsule. High levels of prostaglan‐din E2 (PGE2) and interleukin‐6 (IL‐6) were detected in the cyst fluids. In conclusion, active bone resorption may contribute significantly to the growth of these lesions with

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01170.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The aim of the present study was to investigate epithelial cell proliferation in the linings of odontogenic cysts, including three different subtypes of odontogenic keratocyst (OKC), namely simple (non‐recurrent), recurrent and basal cell naevus syndrome (BCNS)‐associated lesions. Ki67 immunoreactivity in OKC (simple, n = 10; recurrent, n = 8; syndrome, n = 9), dentigerous cysts (DC, n = 5), radicular cysts (RC, n = 5) and normal oral mucosa (n = 7) was studied using a biotin‐streptavidin‐peroxidase method on paraffin sections after microwave treatment. Ki67+epithelial cells were counted manually and related to the length of basement membrane (BM) as determined by TV image analysis. Data were analysed by the Mann‐Whitney U test. The number of Ki67+ cells in simple OKC linings (53.1 ± 17.8 cells/ mmBM) was similar to that in oral epithelium (42.5 ± 12.7 cells/mmBM; P>0.2). However, both contained significantly more Ki67+ cells than DC (3.9 ± 1.3 cells/ mmBM) and RC linings (6.7 ±4.8 cells/mmBM; P<0.006). The epithelial distribution of Ki67+cells differed between groups, with the percentage of positive cells in basal layers in OKC linings (7.0 ± 2.1%) being significantly lower than that of oral epithelia (35.9 ± 5.6%), DC (78.4 ± 8.4%) and RC (80.6 ± 7.7%) linings respectively (P0.3). However, OKC associated with BCNS contained significantly higher numbers of Ki67+ cells (91.8±35.6 cells/mmBM; P<0.01). There was no difference in epithelial distribution of positive cells between the OKC groups. The results are consistent with OKC having a greater proliferative capacity than DC and RC and that its recurrence is not associated with a subgroup of lesions exhibiting increased proliferation. The increased proliferation in OKC associated with BCNS presumably reflects the underlying genetic defect(

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01171.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

One hundred and eight ameloblastomas diagnosed in a rural black Africa population were analysed for clinicopathologic findings other than those classically described. One patient had a polycystic ameloblastoma adjacent to an ameloblastic fibroma. Two other polycystic ameloblastomas showed aneurysmal bone cyst formation and one mandibular tumour was diagnosed as a keratoameloblastoma. Microscopic changes resembling an adenomatoid odontogenic tumour were present in association with two unicystic ameloblastomas and a HPV18‐positive verrucous lesion occurred in the lining of a cystic space of a polycystic ameloblastoma. Two ameloblastomas contained eosinophilic granules in all tumor cells and melanocytes were diffusely present in another. One case exhibited a focus of mucous cell metaplasia. Two polycystic ameloblastomas showed diffuse interstitial ossification. One mandibular tumor was diagnosed as a desmoplastic ameloblastoma and another as an odontoameloblastoma. This study demonstrated that although ameloblastomas are regarded as a fairly homogeneous group of neoplasms, detailed investigations prove clinicopathologic diversity in a significant number of lesion

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01172.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The results of a histological and histochemical study of an ameloblastic fibroma in the maxilla of a young cat are presented. Staining with Picrosirius red revealed the presence of collagen fibers of abnormal packing in the ectomesenchy‐mal component of the tumor. A discussion relating to the classification of the tumor with reference to those in humans is presente

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01173.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The fungusFusarium moniliformecauses fusariosis, which can be invasive and fatal in immunocompromised patients. We report a case of oralFusariuminfection in a granulocytopenic patient with acute myelogenous leukemia who developed necrotic ulceration of the gingiva, extending to the alveolar bone, but was otherwise free of any active systemic lesions.Fusarium moniliformewas identified, by histopathology and culture, to be present in the lesion and was deduced to be the causative organism for this invasive oral infection.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01174.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY