摘要:

Shuler CF, Mostofi R, Lin L‐M, Schwartz SA. Keratin protein identification in dimethylbenzanthracene‐induced hamster cheek‐pouch squamous cell carcinomas. J Oral PatholIndividual keratin proteins were identified in 10 DMBA induced squamous cell carcinomas (SCC) of the hamster cheek pouch. SDS‐polyacrylamide gel electrophoresis of water‐insoluble cytoskeletal extracts from the tumor tissue demonstrated alterations in the protein distribution normal for the site. Immunoblot analysis with a broad spectrum polyclonal antikeratin antiserum identified the keratins in the preparations and confirmed changes in their distribution in the tumor preparations. The major keratin species for all the tumor tissues ranged in molecular weight from 45 to 57kd. The normal tissues had keratins with molecular weights from 45 to 73kd. The absence of high molecular weight keratins was a prominent feature in all the cancers. The histologic appearance of the tumors was varied but the distribution of the keratins was not correlated with the various histologies. The results demonstrate that changes in keratin gene expression occur in DMBA‐induced cheek‐pouch carcinomas but the precise alterations in the keratin proteins from those seen normally are no

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1987.tb02059.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Abstract—Tooth eruption is a localized, bilaterally symmetrical series of events which involves resorption and formation of alveolar bone on opposite sides of the tooth and requires the presence of the dental follicle. We examined the effect on eruption of selective surgical removal of parts of the follicle. Removal of either the basal or coronal halves of the follicle prevented eruption. Bone resorption and formation of an eruption pathway did not occur after removal of the coronal part of the follicle and bone formation did not occur after removal of the basal part of the follicle. Exposure and incisions of the follicle had no effect on eruption. We interpret these data to mean that the polarized resorption and formation of alveolar bone that occur around a tooth during eruption are regulated by the adjacent parts of the dental follicl

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1987.tb02060.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Abstract—Nine cases of homogenous leukoplakia and 21 cases of lichen planus (11 reticular and 10 erosive) were studied under the electron microscope. The changes found in leukoplakia were limited to occasional breaks in the basal lamina and modest changes in the cytoplasm of the basal cells. The basal lamina in lichen planus was found to exhibit 3 distinctly different pictures reflecting the clinical types of lichen planus. The structural abnormalities in the basal cells increased with increasing severity of the lesions. The inflammatory infiltrate in lichen planus was found to contain mainly small‐to‐medium‐sized lymphocytes but plasma‐cells with widely dilated rough endoplasmic reticulum containing a granular substance were frequently seen. Similar changes are seen in other disorders and, therefore, specific diagnostic criteria cannot be established on the basis of the present

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1987.tb02061.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1987.tb02062.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Presidential address presented by Dr. B. G. Radden at the Opening Ceremony of the Biennial Congress of the International Association of Oral Pathologists held in association with the British Society for Oral Pathology in Edinburgh, Scotland in September 1986.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1987.tb02063.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1987.tb02064.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

The most commonly used immunocytochemical techniques and their applications are described. Problems relating to retention of tissue immunoreactivity and specificity of antibodies are discussed with special reference to the effects of tissue processing, presence of cross‐reacting antibodies and lineage non‐specificity of monoclonal antibod

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1987.tb02065.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1987.tb02066.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Abstract—Despite the apparent complexities of vocabulary and techniques involved in nucleic acid hybridization, these methods should become important new weapons in the pathologist's armoury. The particular strengths lie in the analysis of genes whose protein products defy detection either because they are absent (due, for example, to cellular controls as found in viral latency), to a point mutation, or to gene deletion (as is often the case with cellular oncogenes). However, the mere application of this technology will not solve all diagnostic problems. One must be aware, particularly in applyingin situhybridization to a new system, of the artefactural binding of the probes, and suitable control and duplicate experiments should be performed. In addition, it is vital to verify the identity of the probes and the specificity of the reaction by filter hybridization. The latter procedure may seem unwieldy, but is, in fact, no more complex than the recommended procedures for immunohistochemistry, as discussed by Matthews (this symposium). Sadly, for both hybridization and immunological detection, operator inexperience has often prevented such checks of specificity and as a result many spurious results populate the scientific literature, for example a recent controversy over the detection of Human T cell Lymphotrophic Virus Type 1 in multiple sclerosis patients (31). With careful application, however, these techniques will permit the detection of viral molecules under conditions where traditional electron microscopy/histology have failed, and may reveal possible viral aetioligies for a range of hitherto non‐viral disea

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1987.tb02067.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY

摘要:

Recent progress in understanding the biology of keratins together with the development of monoclonal antibodies to individual keratin proteins provide the foundation for studying keratin expression in normal and pathological oral epithelia. Areas of oral pathology in which the examination of epithelial keratin profiles may yield information of potential diagnostic value are discussed. Examples of altered keratin expression in epithelial dysplasia, oral cancer and odontogenic cysts and tumours are presented. Immunocytochemical demonstration of individual keratins can clarify the composition of complex epithelia and may help to establish epithelial lineage or indicate progression changes in tumours. Some problems in the application and interpretation of keratin immunocytochemistry are also considered.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1987.tb02068.x

出版商:Blackwell Publishing Ltd    

年代:1987

数据来源: WILEY