摘要:

The present study compared the alkaline phosphatase (ALPase) expression and DNA content at specific periods in cultured cells derived from non‐inflamed enlarged gingivae of idiopathic gingivofibromatosis (IGF) and phenytoin‐induced hyperplasia (PHG). Cultured cells from healthy gingiva or periodontal ligament (PDL) were used as controls. The DNA assay, ALPase assay and cytochemical staining for ALPase in cultured cells were performed at four, seven, and nine days. The presence of intense ALPase activity was a prominent feature in cultured IGF cells, whereas very low ALPase activity was detected in PHG cells. The cell lines tested showed no significant differences in DNA content. The expression of ALPase in these cells was population density‐dependent. The observation that cells isolated from both types of gingival overgrowth exhibited a different ALPase profile at variance with normal gingival fibroblasts suggested that a distinct pathogenic mechanism may be involved in each type of gingival overg

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01147.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Cleft lip, with or without cleft palate, is one of the most common defects in craniofacial formation. The primary palatogenesis of mice is similar to that of humans and spontaneous cleft lip is associated with genotype in both mice and humans. To investigate the temporal and spatial expression ofrasgenes in cleft (A/WySn) and non‐cleft strains of mice (BALB/cBy), a broad spectrumrasantibody was used. Positive staining was found in ectodermal, mesenchymal, and neuroepithelial cells of facial prominences before the primary palate formation stage (10 d 20 hr) in both strains. During the primary palate formation stage (11 d 20 hr), positive staining was found in the ectodermal and mesenchymal cells of the facial prominences of the non‐cleft strain but not in those of the cleft strain. These results suggestrasgenes may play a role in the primary palatogenesis of mice. Cleft lip could be associated with the deficiency ofrasgene expression during primary palate formation of m

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01148.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Epstein‐Barr virus (EBV) DNA was detected byin situhybridization at 3 sites of 30 samples taken from clinically normal lateral border of tongue mucosa from 15 AIDS autopsies and in none of 20 samples from 10 controls. The first positive case showed a thin layer of parakeratosis correlated with positive signals for EBV in one area and an adjacent area without obvious parakeratosis was also positive for EBV. These findings were present on both sides of the tongue. The second case was unilaterally positive for EBV and parakeratosis was absent. The hybridization signals were localised to koilocyte‐like cells in the stratum spinosum, as in oral hairy leukoplakia (OHL). These observations suggest that thein situhybridization technique can detect very early or subclinical OHL, and supports the role of EBV in the pathogenesis of this les

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01149.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The purpose of this study was to undertake preliminary analyses of the extracellular proteoglycans in carcinogen [4‐nitroquinoline N‐oxide (4NQO)]‐treated rat tongue mucosa. Experimental rats were exposed to twice‐weekly applications of 4NQO in propylene glycol for six months, after which the animals were killed. Control and 4NQO‐treated tissues were subjected to sequential aqueous extractions of proteoglycans under associative and dissociative conditions, followed by alkaline cleavage of protein‐glycosaminoglycan linkages to yield a glycosaminoglycan residue. Tissues subjected to 4NQO applications contained smaller proportions of proteoglycans which were readily soluble under associative and dissociative conditions. Proportionately more proteoglycan remained strongly associated with other intercellular tissue components, being released only by alkaline cleavage. These biochemical alterations in preinvasive 4NQO‐treated epithelium and connective tissues, together with an observed associated change in water retention by the connective tissue, occurred prior to actual neoplastic invasion and suggest differences in macromolecular conformation and orderliness. We hypothesize that these changes are related to the phenomenon of neoplastic epithe

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01150.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

To determine whether the local administration of 9,10‐dimethyl‐1,2‐benzanthra‐cene (DMBA) into the hamster maxillary sinus induced carcinoma at the injected site, hamsters were injected with 30 μl of 0.5% solution of DMBA in dimethyl sulfoxide (DMSO) through the infraorbital foramen into the maxillary sinus once weekly for 10 weeks (Group 2). Another group of hamsters (Group 1) received similar injections of 30 μl of DMSO only. In a third group of animals (Group 3), a roll of oxycellulose was inserted into the maxillary sinus and 40 μl of a 2% solution of DMBA in DMSO was injected once. Sinonasal carcinomas were demonstrated in 73% (8/11) of the hamsters in Group 2 and sarcomas were shown in 73% (8/11) of the hamsters in Group 3, as well as some carcinomas. No tumors were seen in the Group 1 hamsters. Histologic examination revealed squamous cell carcinomas arising from the surface epithelium and submucous glands of the nasal cavity and maxillary sinus. These findings indicate that the intrasinal administration of a 0.5% solution of DMBA in DMSO is a reliable method for inducing maxillary si

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01151.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The masseter and temporalis muscles were investigated ultrastructurally in 2 patients having fibrodysplasia ossificans progressiva (FOP). Results showed atrophy and necrosis of muscle fibres. Both cases contained cartilage which was calcified in the second case and was associated with degenerating chondrocytes. The blood vessels showed various changes, with haematoma formation and large fibrin deposits. Some of the fibrin deposits seen in the second case were surrounded with cartilage. This suggests that the cartilage probably forms as a reaction to an old haemorrhage. It is possible that the effect of the mutant gene found in FOP is on blood vessels rather than the normal regulatory mechanisms of the inducible osteogenic precursor cells. These degenerating blood vessels often result in haemorrhage and fibrin deposits; the fibrosis, cartilage or bone formation are probably secondary changes. Amianthoid fibres found in the tendon of the temporalis muscles of the second case, together with the other changes of fibrosis and cartilage formation, account for the severe restricted mouth opening suffered by these patients.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01152.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

The emerging synthesis of glycoconjugates containing specific oligosaccharides in developing human fetal labial and lingual salivary glands has been investigated by lectin histochemistry. An avidin‐biotin technique was used to study the binding of lectins fromUlex europeusI (UEA‐I),Dolichos biflorus(DBA),Glycine maximus(SBA),Helix pomatia(HPA),Arachis hypogaea(PNA) andTriticum vulgare(WGA) to specific sugars on sections of tissue from labial glands, glands of Blandin and Nuhn, glands of von Ebner and the dorsoposterior lingual salivary glands. Incipient synthesis of glycoconjugates in early glands and their presence in the cells and ducts of the later glands was shown. The study also showed a time‐related increase in both staining intensity and binding sites of serous acinar cells from all glands and for all lectins used. For mucous cells, peak intensity of staining was reached by the middle phase of development. During later gland development this intensity was maintained in dorsoposterior lingual glands but tended to decline in labial glands. The various lectins showed different degrees of binding but UEA‐I lectin generally bound the L‐fucose sugar group in all salivary glands at all gestational ages. The results showed that lectins appear to bind to the oligosaccharides on epithelial cell surfaces of fetal salivary glands at all stages of development. The degree of change depends upon the stage of differentiation and maturation of t

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01153.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Glycogen storage disease type 1b is a rare metabolic disorder which affects the transport system of glucose‐6‐phosphatase metabolism. As a result, hepatomegaly, failure to thrive, renal dysfunction and recurrent infections occur in affected patients. In this paper, the oral complications in three children with glycogen storage disease type 1b are discussed. Oral ulcers were a common rinding, probably due to severe neutropenia and impaired neutrophil migration which characterises the onset of this rare disor

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01154.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY

摘要:

Oral manifestations of infantile systemic hyalinosis in a child of Asian origin are presented. Infantile systemic hyalinosis is a rare fatal condition with probably an autosomal recessive mode of inheritance. The symptoms become apparent soon after birth and death usually occurs before the age of two years. The systemic features are essentially due to widespread deposition of hyaline material in tissues. These include thickening and nodularity of skin, growth failure, joint contractures, osteoporosis, diarrhoea and recurrent infections. The oral changes in the case reported here included thickening of the oral mucosa, extensive overgrowth of gingival tissue, osteoporosis, marked curvature of the dental roots, and replacement of periodontal ligament by hyaline fibrous material. Immunohistochemistry revealed widespread presence of Type VI collagen in the connective tissue with particularly intense staining in the hyaline material.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1995.tb01155.x

出版商:Blackwell Publishing Ltd    

年代:1995

数据来源: WILEY