ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1984.tb01418.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Carbohydrate analyses were performed on dental plaque collected from the teeth of irradiated monkeys, non‐irradiated monkeys and a group ofStreptococcus mutansfree animals, all of which were fed the same standard cariogenic diet. Glucose was the predominant sugar constituent in plaque and was detected in highest concentration in the irradiated animals. Small amounts of pentoses and other hexoses were also present. Plaque from irradiated animals contained, by comparison with the other groups, higher levels ofStrep, mutansand lower levels ofStreptococcus sanguisand Actinomyce

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1984.tb01419.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Surface markers of human gingival fibroblastsin vitrowere investigated using monoclonal and helerologous unlisera against a range of cell surface antigens, together with resetting techniques to characterize surface receptors for IgG and C., WI‐38 fibroblasts and human peripheral blood monocytes were used as control cells. Human gingival fibroblasts exhibited complement receptors and (β2‐micro‐globulin, as did W1–3S cells. Ten per cent of the human gingival fibroblasts were positive for HLA‐DR antigens and additionally exhibited a granulocyte antigen not apparent on WI‐3K cells. Monolayers of the gingival fibroblasts were further exposed for short periods to varying concentrations of enzymes (trypsin, collagenase and neuraminidase), bacterial extracts (lipopolysaccharide and lipoteichoic acid) and crude supra‐ and subgingival plaque sonicates. Surface‐marker analysis was then carried out. The most noticeable effects were obtained withVibrio choleraeneuraminidase which enhanced C3, receptor and surface antigen expression, and supragingival plaque sonicate which depressed the expression of HLA‐DR and granulocytc antigens while not affecting β2microglobulin expression. Trypsin reduced antigen expression to a degree, but its effects were mainl

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1984.tb01420.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

One of the features of epithelial dysplasia at the histological level is known as “loss of cellular adherence” in which adjacent epithelial cells appear more widely separated from each other than in normal tissues. In this study we examine the effects of the carcinogen DMBA on the epithelium of the hamster cheek‐pouch with particular emphasis on the dimensions of the intercellular spaces. DMBA‐induced lesions were processed for electron microscopy and assigned to hyperplasia, dysplasia and carcinoma groups, using defined criteria on toluidine blue‐stained 1 urn Araldite sections. Untreated pouches were used as a control. At the light‐microscopical level, intercellular spaces in hyperplastic epithelium appeared similar to those present in untreated tissue but increased progressively in dysplastic and car‐cinomatous lesions. Spaces were generally wider between basal and spinous cells than between granular cells, although wide variations were observed between tissue blocks demonstrating similar histological features and also within adjacent areas of the same block. At the ultrastructural level, untreated and hyperplastic tissue showed only occasional focal separations of adjacent plasma membranes; these spaces were more frequent between cells of lower strata. In sections from dysplasia and carcinomas, spaces were always extensive and were occupied by numerous villous or foliate membrane‐bound cytoplasmic extensions. These were often attached to each other by desmosomcs of apparently normal morphology but of a lower frequency than in untreated epithelium. The increased epithelial separation as indicated by the increased intercellular spaces during chemical cardnogenesis may be a result of any or all of the following factors: desmosomal disruption or their failure to develop; the production of cell‐surface molecules which are less adhesive; inflammatory oedema and direct alterations on intercellular junctions and cell‐surface components by infiltrating

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1984.tb01421.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

The present report investigated the extent of the epithelial dysplastic feature known as “loss of cellular adherence” at the ultrastructural level by quantifying the volume of the intercellular space during hamster cheek‐pouch carcinogenesis. Following topical application of DMI3A to cheek‐pouches, lesions were classified as hyper‐plasia, dysplasia and carcinoma, with untreated pouches serving as a control. Stereological point counting procedures were used to determine the volume density of intercellular space in defined basal, spinous and granular layers for each group. In general, progressive increases in volume density were detected within each stratum during carcinogenesis. These results indicate that increasing separation of epithelial cells occurs during carcinogenesis, although it is not yet known whether this results from loss of cohesion between specialised (i.e., desmosomal) or non‐specialised membrane areas. In addition, a simple indicator of pathological alteration, the Pathological Alteration Ratio (PAR), is described and was used to evaluate existing published data for intercellular spaces in various oral mucosal conditions. Values of the PAR were found to be substantially higher in carcinogen‐treated epithelia than in reports describing changes in wound healing, lichen planus and leukoplakia simplex. These objective techniques are of value for investigating the pathogenesis of diseased epithelium and may find applications in the diagnosis of oral premali

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1984.tb01422.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

This paper describes the ultrastructural, topographical changes seen in rat gastric mucosa following application of 0.1 N HC1 or 1,10 or 50 niM NaF in 0.1 N HC1 to the stomach. No morphological differences were noticed between the 0.1 N HC1 (control) and 1 mM NaF in 0.1 N HCI specimens. Ten milliomolar NaF in 0.1 N HC1 produced some desquamation of surface mucous epithelial cells while 50 mM NaF produced extensive damage to cells surrounding the gastric gland openings (foveolae) as well as inlerfoveolar cell loss.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1984.tb01423.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

To find out whether smoking affects the prevalence and intraoral distribution ofCandida albicans, swabs and saliva samples from 100 healthy persons, smokers and non‐smokers, were cultured for the presence of this fungus. The prevalence was the same (35%) in both smokers and non‐smokers. Among carriers, the mean concentration ofC. albicanscolony‐forming units in saliva of smokers was twice that of the non‐smokers, and the isolation frequency ofC. albicansat each of 5 mucosai sites was also higher in smokers than in non‐smokers. However, a wide variation was found, and these differences were not significant at the 0.05 level. Men were carriers more often than women (p<0.025), and the mucosal site from whichC. albicanswas recovered most often was the posterior dorsum of the tongue. Although it has previously been claimed that cigarette smoking influences the carrier state ofC. albicans, the present study suggests that the effect is on

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1984.tb01424.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Electron microscopic, histochemical and light‐microscopic studies were carried out on biopsy specimens of 12 eases of leukoedema and on the desquamated epithelial cells of a further 50 examples. For comparison, similar observations were made of 4 examples of healthy cheek mucosa and 29 cases of a variety of pathological conditions of the oral mucosa. Only in leukoedema were a series of structures encountered which consisted of an aggregation of ribosomes and electron dense masses, or a network of electron‐dense material, or an electron‐dense network with a central dense core or a solid body with a few central cavities. Histochemical stains and RNA extraction studies showed that they are probably abnormal forms of keratohyalin granules and that ribosomes is an important component of their compos

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1984.tb01425.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

Fibrous histiocytomas of the oral and maxillofacial regions arc rare. The present study analyzes a sample of 63 cases from the literature together with 7 personally observed cases. Age, sex, site, size, clinical behaviour, treatment and survival were evaluated and related to a histological classification based on that of Rosai (1981). Lesions were assessed for histological criteria which would place them in one of 2 groups: fibrous histiocytoma (FH) or pleomorphic fibrous histiocytoma (PFH). Within these 2 histological groups the cases were subdivided into clinically benign, aggressive or malignant lesions. Thirty‐nine cases (56.0%) were classified as FH and 31 eases (44.0%) as PFH. The mean age of male patients with PFH was significantly higher than female patients with FH (t = 3.05; d.f. 37; p<0.0025). Lesions in the PFH group involved bone more frequently than those in the FH group (Yale's Chi2 = 16.66; d.f. 1; p<0.00025). Lesions involving bone for both histological groups were more likely to be aggressive or malignant than soft‐tissue lesions (Yale's Chi2 = 29.9; d.f. 1; p<0.00025). Soft‐tissue lesions were usually less than 5 cm in greatest diameter. Radiographic features of malignancy for lesions with bone involvement is of prognostic importance regardless of the histological appearance of the lesion. The majority of the lesions under study were from the deep tissues of the oral and maxillofacial re

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1984.tb01426.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY

摘要:

In 2 parotid adenolymphomas, the composition of lymphoid stroma was analysed, using Stereologic point‐counting methods at the electron microscopic level. In the 2 lesions, the stroma was almost identical with respect to organization and composition. Most areas of the stroma showed accumulation of mixed cell populations. On average, the latter were comprised of 5.1 % fibroblastic/relicular cells, 2.7% macro‐phages, 68.9% small and 18.8% medium/large lymphocytes, 0.5% blastforming T‐lymphocytes, 2.3% blastforming 13‐lymphocylcs, and 1.6% plasma cells. Polymorphonuclear leukocytes and mast cells were extremely rare. Areas corresponding to the mantle zone of lymphoid follicles were found to contain 83.0% small and 15.8% medium/large lymphocytes. The present data seem to be in accordance with the speculation that the lymphoid stroma of adenolymphoma represents an immune r

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1984.tb01427.x

出版商:Blackwell Publishing Ltd    

年代:1984

数据来源: WILEY