ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1983.tb00353.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The recovery of the rat submandibular gland after retrograde infusion of water‐soluble radiographic contrast medium was studied using an experimental model. During continuous monitoring of the developing intraglandular pressure, the glands were subjected to ductal and slight parenchymal filling or heavy parenchymal filling with the medium. The animals were killed after varying recovery periods, and the tissue was prepared for light and electron microscopic examination. Dilation of the ductal lumina, induced during ductal and slight parenchymal filling, was successively reduced and, generally, the parenchyma had a normal appearance at 30 h. In glands subjected to heavy parenchymal filling, the changes in the intralobular ducts were more pronounced and were also seen at later times after infusion. Alterations in the acini, comprising fusion of secretory granules, vacuole formation and dilation of the acinar lumina and intercellular canaliculi, were observed. At later times, atrophy of the parenchymal cells occurred together with an apparent proliferation of the connective‐tissue stroma, as well as an increase in the number of small blood vessels. An inflammatory cell‐infiltrate was seen in both groups of animals, but was most prominent in glands subjected to heavy parenchymal filling. The infiltrate, comprised primarily of macrophages and polymorphonuclear leukocytes, reached a peak at 20 h after infusion. At later times, mast cells and occasional eosinophils were seen. The observed alterations and the pattern of recovery are most likely due to the induced intraglandular pressure and the following inflammatory reaction. It is also possible that the changes, to some extent, are influenced by the presence of the contrast medium in the t

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1983.tb00354.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Lipid‐soluble radiographic contrast medium was infused retrogradely through the main excretory duct of the rat submandibular gland until ductal and slight parenchymal filling or heavy parenchymal filling was achieved, as determined by the developing intraglandular pressure. The glandular tissue was fixed by vascular perfusion at different times following infusion, and examined by light and electron microscopy. Dilation of the intralobular ducts and scalloping of the luminal border were pronounced after both degrees of filling. Widening of the acinar lumina and intercellular canaliculi occurred early. Masses of fused secretory granules were commonly seen in the cytoplasm of the acinar cells and formation of vacuoles occurred frequently. In some acinar cells, densely packed granules filled the major part of the cytoplasm. Large lysosomes and autophagic vacuoles, frequently present in the various parenchymal cells, often contained apparent contrast medium. In addition, widening of the intercellular space between parenchymal cells was seen, particularly in the acini and intercalated ducts. An inflammatory cell‐infiltrate, primarily comprised of polymorphonuclear leukocytes, was particularly prominent at 20 h and 30 h after infusion. The changes were generally more pronounced and persisted for a longer time in glands subject to heavy parenchymal filling. Large cyst‐like spaces surrounded by inflammatory cells were seen in the lobules and in the interlobular connective tissue of these glands. At later times, areas containing apparent contrast medium surrounded by macrophages, were frequently observed in the connective‐tissue stroma. Atrophy of the parenchymal cells was seen later, after heavy parenchymal filling, and a proliferation of the connective tissue had occurred. The changes were most probably caused by the elevated intraglandular pressure induced during infusion, and the subsequent; cell damage and inflammatory reaction. A foreign body reaction, induced by the retained lipid‐soluble contrast medium, was probably partly responsible for the morphological alterations observed, following

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1983.tb00355.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Using the G‐banding technique the chromosomal patterns in 10in vitro‐cultured human mixed salivary gland tumours were studied. When surveying the results from the present 10 adenomas together with previous results from 31 adenomas, the following characteristic features were found. Mixed tumours probably always originate with a normal diploid stemline. This stemline type was also found to predominate during progression. However, about 41% of the tumours showed abnormal stemlines characterized by highly specific, usually structural, clonal aberrations. The deviations in these abnormal stemlines seemed to preferentially affect certain chromosome types, viz. Nos. 8 and 12. This non‐random pattern of deviations ‐ not found earlier in either benign or malignant tumour types in man ‐indicates that certain regions of, especially, chromosomes 8 and 12 are selectively affected in mixed tumours. Among other things, changes of gene dosage and/or oncogene activation are mentioned as factors possibly influencing the characteristics of this non‐ran

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1983.tb00356.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Usingin vitromethods, the G‐banding pattern in a highly differentiated adenocarcinoma of the parotid gland is described. Results from two consecutive preparations of the carcinoma indicated that it had originated with a normal diploid stemline and, subsequently, during progression had developed an aneuploid stemline deviating from the normal only by loss of the Y chromosome. From this hypodiploid stemline some clones and variant cells had been generated, all of them characterized by numerical deviations, particularly various trisomies. Important cytogenetic similarities were found between the present adenocarcinoma and other types of salivary gland neoplasms, in particular pleomorphic adenoma

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1983.tb00357.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

T and B lymphocyte (sub)populations were identified by monoclonal hybridoma antibodies (the avidin‐biotin‐peroxidase complex method), in the periductal lymphocyte‐rich infiltrates in the labial salivary glands of 8 patients with secondary Sjögren's syndrome (2°SS) associated with systemic lupus erythematosus (SLE). 59 ± 7% and 17 ± 3% of inflammatory round cellsin situwere T3‐positive and surface(SIg)‐ or cytoplasmic(CIg) immunoglobulin‐positive, respectively. This suggests a local T lymphocyte dominance in salivary glands in 2°SS associated with SLE. The local ratio of cells expressing T inducer/helper: T suppressor/cytotoxic phenotype was 3.5 ± 0.8 (range 0.9–7.6) indicating large variations between individual patients. 46 ± 9% of all inflammatory cellsin situwere endogenous peroxidase‐negative, Ia‐positive cells, suggesting an active role for the locally a

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1983.tb00358.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Twenty‐five patients with chronic oral lichen planus, usually of the atrophic‐erosive type, were treated for 2 months with Etretinate® 0.6 mg/kg b.w./day, followed for 4 months by Etretinate® 0.3 mg/kg/day, or 0.1% Tretinoin® in an adhesive base. Complete resolution or improvement was seen in 85% of the lesions after first treatment. The number of patients with oral soreness and pain was significantly reduced (p<0.001). During the second treatment, the improvement was maintained or even increased in about 70% of the patients, irrespective of the mode of treatment. Treatment was discontinued in one patient owing to moderately increased serum transaminase levels. The number of drug‐related drop‐outs was significantly lower than in a previous study utilizing an etretinate dose of about 1 mg/kg/day (p<0.05). Other adverse effects were minor and tolerable. Retinoids offer an effective mode of therapy for severe oral lic

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1983.tb00359.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

Tyrosine‐rich crystalloids are described in an example of an adenoid cystic carcinoma arising in an oral minor salivary gland of the hard palate. This represents the first reported case of an adenoid cystic carcinoma of oral minor salivary gland in which tyrosine‐rich deposits have been identified. The tyrosine deposits appear refractile but not birefringent at the light microscopic level; and histochemical findings indicate a relatively simple, homogeneous chemical composition rich in tyrosine. Their ultrastructural nature is that of a finely granular, but otherwise patternless, highly electron‐dense material. The electron microscopic features of the neoplasm are similar to previous reports. Based upon the findings of this and previous investigations, it is not as yet possible to state whether the tyrosine‐rich deposits truly represent a crystalline or amorphou

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1983.tb00360.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

A large single dose (15 mg/kg body wt, ip) of the antitumor agent adriamycin (ADR) caused a marked increase in calcium concentration of submaxillary gland of female mice, and a smaller increase in the parotid gland within 2 days of injection. A small dose (2.5 mg/kg body wt) had no effect. The histological appearance of the glands was also changed and included an increase in size of granules and acinar cells of the submaxillary glands and a decrease in size of acinar cells of the parotid. At the EM level, there was evidence of mitochondrial alteration in the parotid but not in the submaxillary glands. Rough endoplasmic reticulum (RER) was markedly disorganized in the parotid, and abnormal whorls of RER were evident. Submaxillary glands showed no change in RER. Water content of either gland was unchanged from that of controls. Heart ventricles, unexpectedly, showed no change in calcium concentration from that of control tissues, at 3 h, 1, 2 or 4 days after ADR administration. The [Ca] changes induced by ADR in the submaxillary glands are not mediated via β‐adrenoceptor activation since propanolol did not alter the ADR‐induced changes. The marked difference in response of the glands (and heart) to ADR, suggests that the mechanisms invlolved in calcium homeostasis in these organs are very diffe

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1983.tb00361.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY

摘要:

The ultrastructural and enzyme cytochemical features of two follicular ameloblastomas were investigated. The peripherial cells of the follicular areas in both lesions had several types of tall columnar cells which were highly polarized and showed varying degrees of cellular differentiation. These polarized cells had their nuclei situated away from the basal lamina, and often contained dilated strands of endoplasmic reticulum in the subnuclear cytoplasm. Some of these cells also contained dense‐cored secretory granules, condensing granules and coated vesicles in the cytoplasm adjacent to the basal plasma membrane. These cells bore a striking resemblance to pre‐ameloblasts and early secretory ameloblasts. Alkaline phosphatase and ATPase cytochemistry supported these morphologic observations. Interestingly, the central cells of the follicular areas were consistently negative for alkaline phosphatase activity as were the peripheral cells, while both cell types had ATPase activity demonstrable at their cell surf

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1983.tb00362.x

出版商:Blackwell Publishing Ltd    

年代:1983

数据来源: WILEY