摘要:

An international group of epidemiologists, clinicians and pathologists with a special interest in oral white lesions and their precancerous significance has reviewed earlier work on this topic and identified some of the problems associated with previous definitions, descriptions and classifications. Modifications to these definitions, descriptions and classifications have been proposed, accompanied by explanations of the reasons for identifying the need for changes to be made. Leukoplakia may be a provisional or definitive diagnosis dependent upon the circumstances of oral examination and the availability of other information. Guidelines are provided to assist in the application of the definitions of oral leukoplakia and illustrations depict the homogeneous and non‐homogeneous clinical variants. Consideration is also given to the importance of a red component in a white lesion, or a lesion that is entirely red (erythroplakia). A new clinical staging procedure for oral leukoplakia is also propose

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00191.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Sixty squamous cell carcinomas of tongue and buccal mucosa were examined for expression of p53 protein by using an immunohistochemical technique improved by an antigen retrieval method. Twenty‐seven (45%) tumors demonstrated strongly positive staining. Thirteen of p53‐positive tumors (48%) also exhibited Overexpression of p53 in immediately adjoining hyperplastic or pre‐malignant epithelium. All 22 metastatic lymph nodes and 18 local recurrent lesions (except two) had an identical p53 immunophenotype to their corresponding primary sites. Mitotic indices were significantly higher in p53‐positive tumors (P0.1). There was no correlation between p53 Overexpression and tumor grade, size and staging, vascular invasion, lymph node metastasis, and early local recurrence. Overexpression of p53 was found to be relatively higher, although not statistically significant, in nonsmokers than in heavy smokers (66.7% vs. 42.9%). and in non‐betel‐quid chewers than in heavy chewers (62.5% vs. 34.2%). These data are consistent with the hypothesis that inactivation of p53 protein may occur in the early phases of oral tumorigenesis. It may not be a useful prognostic marker but could possibly be used for risk assessment and surveillance of local

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00192.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

The tumour suppressor gene p53 is frequently mutated in neoplasia. Since mutant p53 protein is often over‐expressed, mutation can be indirectly detected by immunocytochemical techniques. As microwave antigen retrieval is becoming a widespread method for increasing the antigenicity of paraffin sections, we investigated the application of this technique to p53 immunohistochemical staining of oral mucosa specimens. Paraffin sections of 22 squamous cell carcinomas (SCC) and 36 benign lesions were immunohistochemically stained with and without antigen retrieval. Without antigen retrieval p53 over‐expression was observed in 6/22 SCC and 1/36 benign lesions. Following antigen retrieval positive staining was observed in 15/22 SCC and 35/36 benign lesions. Staining in benign lesions was confined to basal and parabasal cells and could reflect normal functioning of wild‐type p53. We conclude that antigen retrieval increases the sensitivity of p53 immunoreactivity. but such staining is not specific for malignancy and should be interpreted with ca

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00193.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

The purpose of the present study was to determine the minimal arecaidine concentrations showing a synergistic effect on DMBA‐induced hamster cheek pouch carcinogenesis. One hundred and twelve male adult Syrian golden hamsters were divided into 16 groups, each containing seven animals. After eight weeks of DMBA initiation and then four weeks of arecaidine promotion. 100% tumor incidence was found with arecaidine concentrations of 400 μg/ml and 500 μg/ml; average tumor numbers were 1.86 ± 0.63 and 1.86 ± 0.93 respectively (P<0.05). After four weeks of DMBA and a subsequent eight weeks of arecaidine painting, all hamsters developed visible tumors with arecaidine concentrations of 900 μg/ml and 1000 μg/ml: average tumor numbers were l.86 ± 0.82 and 2.14 ± 1.09 respectively (P<0.05). The tumor dimensions varied little and differences were not statistically significant. Without DMBA pretreatment, regardless of the high arecaidine concentrations (1000μg/ml, 2000μg/ml and 3000μg/ml) applied, no visible tumor growth was observed: only hyperkeratosis and inflammation could he discerned histologically. Thus, the minimal concentrations of arecaidine displaying a synergistic effect in the DMBA‐induced hamster cheek pouch of carcinogenesis were found to be 400 μg/ml applied for four weeks after eight weeks of DMBA application, and 900 μg/ml applied for eight weeks after four weeks of DMBA painting. These findings may be useful for other studies concerning the tumorgenici

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00194.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

The prevalence and related risk factors of betel quid chewing among adolescent students were studied in a junior high school (group 1) and in a vocational school (group 2) in southern Taiwan. Group 1 consisted of 3548 participants (89.7% response rate) and group 2 of 1358 (97.6% response rate). The students were asked to complete a questionnaire anonymously. In the junior high school 1.9% of students including all grades (13‐15 years old) and both sexes was found to be a current betel quid chewer and 14% was an ex‐chewer, whereas 10.2% of vocational school students (16–18 years old) was a current chewer and 31% was an ex‐chewer. The prevalence of betel chewing was significantly higher among boys than girls. A high proportion of chewers were also a smoker and alcohol drinker. A statistical analysis of sociodemographic factors showed that male students who smoked tobacco, consumed alcohol and were friends or classmates of students who were betel quid chewers, were the likeliest adolescents to chew bet

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00195.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

An experimental model was established in which the decrease in surface area of CO2laser wounds on rat tongues was measured during the healing process. Scanning electron microscopy was used to measure and delineate the outlines of the initial wounds and those of the residual wounds. A 50% decrease in the surface area of the wounds was observed 10 days after surgery during the healing process. At day 30, the scar surface area was less than 10% (7.4%) of the wound surface area at day 2. This contrasts with studies reported elsewhere where only minimal contraction was observed during the healing of CO2laser wounds. Immunoperoxidase techniques revealed that three different phenotypes of myofibroblasts were present inside the healing area, expressing vimentin, actin and desmin. These persisted until day 30 after CO2irradiation.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00196.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

The purpose of this study was to determine the effect of surgical induction of anterior disk displacement (ADD) on type‐Ill. VI and IX collagens of the rabbit craniomandibular joint (CMJ) tissues using an immunohistochemical technique. The right joint was exposed surgically, all discal attachments were severed except for the posterior discal attachment (bilaminar zone). The disk was then repositioned anteriorly and sutured to the zygomatic arch. The left joint served as a sham‐operated control. Ten additional joints were used as non‐operated controls. Deeply anesthetized rabbits were perfused with 2% buffered formalin 2 weeks (10 rabbits) or 6 weeks (10 rabbits) following surgery. The articular disk, bilaminar zone, mandibular condyle and articular eminence were excised. The last two were decalcified in EDTA. All tissues were then sectioned at 10 um in a cryostat. Sections were incubated with monoclonal antibodies directed against type‐Ill. VI or IX collagens. Following incubation in the appropriate FITC‐fabelled secondary antibodies, all sections were studied under the fluorescence microscope. The results showed a reduction in immunostaining for type‐VI and IX collagens in the condylar cartilage, disk and articular eminence at 2 weeks, followed by an increase in their immunostaining at 6 weeks and the appearance of ade novotype‐Ill collagen in the condylar cartilage and the articular eminence. It is concluded that surgical induction of ADD in the rabbit CMJ leads to alterations in its type‐III. VI a

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00197.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

This paper reports a clinical and roentgenological examination of the teeth, jaws and saliva of 29 Scandinavian individuals with Laurence‐Moon‐Bardet‐Biedl (LMBB) syndrome, whose cardinal signs are retinal dystrophy, polydactyly, obesity, hypogenitalism and mental retardation. All subjects had at least three of these signs, including retinal dystrophy. Compared with normal subjects, the group had statistically significantly higher frequencies of hypodontia. small teeth and short roots. In addition, the saliva showed a buffering capacity higher than normal. In conclusion, there seem to exist disturbances of both dental and skeletal formation in the LMBB syn

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00198.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

An unusual case of polypoid carcinoma in a 72‐year‐old woman is reported. The tumor showed a pedunculated growth on the lateral border of the tongue and was composed of a malignant basaloid component in intimate association with definable foci of epithelial dysplasia of the surface mucosa. It was considered that histlogic features of the present case corresponded with those of basaloid squamous carcin

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00199.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

An ameloblastic fibro‐odontoma which occurred in the mandible of a 3‐year‐old Japanese boy is reported together with immunohistochemical findings. Histologically, the tumor consisted of an ameloblastic fibroma‐like area and some typical complex odontoma‐like areas. The epithelial islands in the ameloblastic fibroma‐like area showed different developmental stages of the epithelial‐connective tissue interface. Immunohistochemical examination revealed that all epithelial components in the ameloblastic fibroma‐like area showed expression of CK 8, CKs 13, 16, CK 14, CK 18 and CK 19, and coexpression of these cytokeratins and vimentin. These findings suggest that even the epithelial component without obvious epithelial‐mesenchymal induction showed the final cell differentiation of the enamel organ with the potential for epithelial‐me

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00200.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY