摘要:

Dentin phosphophoryn is a highly phosphorylated protein which has a hydrophilic character but is not soluble in dilute acetic acid. A histochemical method was developed for staining this protein with Stains‐all® in situ utilizing those chemical properties. We have succeeded in detecting the presence of this protein in circumpulpal orthodentin of human permanent and deciduous teeth, but not in mantle dentin, secondary dentin and reparative dentin. Phosphophoryn staining was also absent in the dentin ofdentinogenesis imperfecta(DI) Type II, a genetic disorder of dentin formation. From these results, it is suggested that phosphophoryn is synthesized and secreted only by physiologically‐differentiated odontoblasts and that the mineralization processes of mantle, secondary, reparative and DI dentins may be different from that of circumpulpal orthode

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00659.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Pharmacogenic pigmentation of the oral mucosa has been reported following the use of a number of anti‐malarial drugs. The nature and distribution of the pigment is inconclusive in the literature. The aim of the present study was to document pigment deposition within the oral mucosa of DA rats following prolonged chloroquine and pyrimethamine administration. The drugs were given as a combined dosage and separately to different groups via stomach gavage tube. After 12 weekly administrations the palatal mucosa was examined histochemically and ultrastructurally for changes in numbers and size of active melanocytes using the dopa‐oxidase technique. The serum was analysed for changes in ACTH and testosterone levels. Morphometric analysis of cells incubated for dopaoxidase showed a significant increase in the size of dopa positive cells with both drugs but an increase in the number of active melanocytes with chloroquine only. Serum levels of ACTH remained unchanged with both drugs but pyrimethaminc caused an elevation in testoster

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00660.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Tissues from patients with oral verrucous carcinoma were examined for the presence of human papillomavirus (HPV). The tissues were stained for the presence of the type common papillomavirus antigen by immunohistochemical staining and the presence of HPV DNA was determined byin situhybridization with biotin‐labelled HPV DNA probes. Seventeen tissue specimens were obtained from 9 patients, and included pre‐malignant lesions and primary and recurrent tumors. One pre‐malignant lesion was positive for papillomavirus structural antigen. This lesion and lesions from 2 other patients hybridized at low stringency (Tm‐35°) to 3 different HPV probes. By hybridization under high stringency conditions (Tm‐20°), the virus in each case was identified as

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00661.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Platelet‐derived growth factor (PDGF), which is released during the “clotting cascade”, has a mitogenic effect on a variety of mesenchymal cells including those of the periosteum. Cell culture provides a method of studying these effects. In the present study, PDGF was studied for its ability to stimulate quiescent calvarium periosteal cells to enter G1, indicated by new protein synthesis. After addition of platelet poor plasma, these cells later entered S phase, indicated by uptake of 3H‐thymidinc. Three hours after PDGF stimulation, there were quantitative increases of at least 18 protein bands as compared to cells left in serum starvation medium. Two of these new protein bands (31K and 45K) required transcription. These data suggest that PDGF in extravasated blood may stimulate appositional bone formation beneath intact periosteum at the site of

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00662.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Biopsies from normal oral mucosa and oral mucosa affected by candidosis, lichen planus or gingivitis were compared with respect to the expression of two Class II transplantation antigens, HLA‐DR and HLA‐DQ, by epithelial cells and the relationship of these antigens to the distribution and frequency of T‐lymphocytes. Indirect immunohistochemistry with different mouse monoclonal antibodies was used on frozen and acetone‐fixed sections. To evaluate the results, a score system based upon the expression of the Class II transplantation antigens by epithelial cells and the frequency of T‐lymphocytes was used. In oral candidosis there was a marked expression of HLA‐DR antigens throughout the epithelium. In addition, this type of epithelium was the only one that expressed HLA‐DQ antigens. An intense intraepithelial infiltration of T‐lymphocytes was observed. Oral lichen planus and gingivitis did, to a much lesser extent, cause the expression HLA‐DR antigens by the epithelial cells. In both lesions, the number of T‐lymphocytes within the epithelium did not exceed the number found in epithelium of normal mucosa. In these types of lesions, the subepithelial infiltrate varied in intensity but was mainly composed of T‐lymphocytes reactive with anti‐Leu 3a antibodies. The results of the present study imply that epithelial expression of the two different Class II antigens arc related to the frequency of the T‐lymphocytes and to the proximity of these cell

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00663.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

One thousand seven hundred and three consecutive cases from a microscopic slide exchange with 10 institutions were evaluated for extent of agreement (or disagreement) defined as: A) complete agreement (85.8%). B) Disagreement ‐difference in diagnostic terminology, no significant pathologic or clinical implications (1.4%); C) disagreement ‐ difference in diagnosis with pathologic significance only (7.7%); C1disagreement ‐ difference in diagnosis with pathologic significance only, radiographic interpretation necessary (1.1%); D) disagreement ‐ major significance for prognosis and/or treatment (4.0%). Trends in disagreement were indentified in the following tissue/location categories: mesenchymal lesions, minor salivary gland tumors, odontogenic tumors, fibro‐osseous lesions, and benign epithelial lesions. These are discussed in relation to the oral pathology literature. All disagreements with significance for prognosis and/or patient treatment were evaluated retrospectively in an attempt to resolve the disparities. The implications for peer review, continuing education, and teaching programs are

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00664.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Ten instances of a white plaque of the lateral tongue unique to homosexual males and referred to as oral hairy leukoplakia were analysed ultrastructurally. The surface epithelial layer exhibited extracellular, intracellular and intranuclear penetration by hyphae ofCandida albicans, sometimes accompanied by coccobacilli in the extracellular space. The subcorneal epithelial layer included koilocytoid ballooned cells which had a paucity of cytoplasmic organelles and displayed condensation and emargination of the chromatin. Cells that exhibited these nuclear changes were found to be infected by a herpes‐type virus which was visualized by electron microscopy in all ten cases. Clusters of nucleocapsids (86‐110 nm in diameter) occurred in the nuclei and enveloped virions (111‐175 nm in diameter) occurred in the cytoplasm and extracellular spaces. Virions showed budding from the nuclear envelope. Bundles of tubular structures (20 nm diameter) arranged in parallel occurred in the cytoplasm of some koilocyloid cells. There was no evidence by electron microscopy of the presence of papilloma virus within koilocytotic n

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00665.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

A histologic and histometric study of the effect of EHDP (ethane‐ 1–hydroxy‐1,1–diphosphonate) on the alveolar wound healing of irradiated animals is presented. This drug was selected based on its osteosclerotic effect when applied at low doses. The three left mandibular molars of 80 male Wistar rats were extracted. The rats were grouped into 4 sets of 20 rats each. Group I received no further treatment; Group II received daily intraperitoneal injections of EHDP (7.5 mg/Kg of body weight during 10 days); Group III: 15 Gy of X‐radiation were applied to the head; and Group IV were irradiated and injected with EHDP as were animals of Groups III and II. Half of the animals were killed after 14 days and the other half 30 days post‐extraction. Sections were made at the level of the mesial socket of the first molar in a buccolingual orientation, on which histometric determinations of bone activity were made. The results showed the attenuation of the inhibitory effect of radiation on bone formation when the animals were treate

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00666.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY

摘要:

Surgical pathology of the head and neck, Vols. 1&2. Barnes L, ed.Review of dental hygiene: Questions and answers.3rd Edition, Steele PEAIDS and the dental team.Greenspan D, Greenspan JS, Pindborg, JJ, Schiødt M

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1986.tb00667.x

出版商:Blackwell Publishing Ltd    

年代:1986

数据来源: WILEY