摘要:

Anxiety and salivary cortiso) were measured in two groups of patients with recurrent aphthous ulceration. One group of patients had persistent aphthae (Group 1) and the others had been relieved of their aphthae following correction of delected haematinic deficiency states (Group 2). Anxiety was measured using the Hospital Anxiety and Depression scale and radioimmunoassay of salivary cortisol. There was a statistically significant increased proportion of borderline or clinically anxious patients in Group 1 compared to Group 2 (P<0.05). Median salivary' cortisol levels also showed a statistically significant elevation in Group 1 (P<0.0 1). It is concluded that stress may play a role in the aetiology of recurrent aphthous stomatitis, particularly in patients who have an underlying anxiety trait.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00278.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Several salivary proteins were assayed in saliva from epileptic patients who were using different anti‐epileptic drugs, viz. phenytoin, valproate and carbamazepine, and were compared with levels in unmedicated healthy control subjects. Flow rate and pH of the patient groups were not different from the controls. In all patient groups the specific amylase activity was increased up to twofold. In the phenytoin group only, the salivary IgA concentration was strongly reduced. Levels of salivary cystatin C were similar among all patient groups studied, and were not different from those of the control group. In contrast, the absolute and relative concentrations of cystatin S were diminished, particularly in patients using either valproate or phenytoin. These data suggest that use of anti‐epileptic drugs over long periods may result in decreased levels of several salivary proteins such as slgA and cystatins. which are involved in the protection of the oral cavity against microbial infecti

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00279.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

A total of 102 rural Cambodian women with a mean age of 60×8.5 years (range 39 to 80 years) who chewed betel quid were examined for oral mucosal lesions, in particular betel chewer's mucosa. The average duration of betel quid chewing was 15.5 × 12.8 years. The average number of daily betel quids was 5.4 × 2.9. Forty women (39.2%) used betel quids overnight. Thirty‐eight (37.3%) did not show any oral mucosal lesion. Sixty‐two (60.8%) showed beiel chewer's mucosa. Homogeneous leukoplakia was found in three women (2.9%). Out of 130 sites affected by chewer's mucosa, the buccal mucosa was the most frequently involed (n= 68). In thirty‐two subjects (31.4%) more than one location was affected. The presence of a lesion was significantly associated with the duration of the habit (P<0.01) and the number of betel quids per day (P<0.001). Betel chewing seems to be prevalent in elderly Cambodian women, while younger people do not seem to have taken up this habit. The risk for oral cancer in the Cambodian population is presently

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00280.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Because betel quid (BQ) chewing has been linked to a higher prevalence of periodontal diseases, the pathobiological effects of arecoline, a main alkaloid found in areca nut, were investigated in cultured human gingival fibroblasts. At concentrations higher than 0.4 mM, arecoline inhibits cell attachment, cell spreading and cell migration in a dose‐dependent manner. These inhibitory effects were associated with intracellular depletion of glutathione (GSH). At concentrations of 0.4 mM and 1 mM. arecoline depleted about 26% and 45% of GSH after 2 h incubation. Exposure of cells to areeoline at concentrations lower than 0.4 mM for 2 h showed no significant decrease in either cell viability or intracellular GSH. However, incubation of cells for 24 h in 1 mM arecoline decreased the cell numbers to only 35% of those in the untreated control. Arecoline also decreased cell growth and collagen synthesis in a dose‐dependent manner. Because of repeated and long‐term exposure to arecoline. BQ chewers could be more susceptible to periodontal damage and less responsive to new attachment proce

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00281.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Inflammatory and developmental cysts of the jaws are relatively common bone destructive lesions in the human maxillofacial skeleton but their pathogenesis is still poorly understood. In this study the role of mast cells (MC), and mast cell tryptase in particular, was evaluated in the pathophysiology of bone resorption and jaw cyst formation in different types of cysts. The distribution of MC and the amount of tryptase in histological tissue sections were determined by immuno‐histochemistry using monoclonal antihuman tryptase antibodies and the results were quantitated by using an image analyzing system. The amount of tryptase was further studied by Western‐blotting and measurement of trypsin‐like activity from the neutral salt extracts obtained from different types of jaw cysts. In contrast to control tissue, high trypsin‐like activities and abundant immunoreactive tryptase were observed in the extracts of all types of cysts studied (radicular, dentigerous and keratocyst). In tissue sections the highest amount of tryptase‐positive staining was observed in radicular cysts (mean 6.2% of reference area) and the lowest amount in keratocysls (mean 2.1% of reference area.>O.O1). MC were found to be located in inflammatory cell‐rich tissue areas and just beneath the cyst epithelium. Importantly, MC located at the border of bone were observed to be degranulated. indicating high activity of MC and release of tryptase at the regions of early bone destruction, Based on previous findings addressing the role of mast eel! tryptase in proteolytic cascades, and the known association of MC with osteoporosis, we suggest that mast cells and mast cell tryptase may contribute significantly to jaw cyst tissue remodelling during growth of a cyst, and to the destruction of the surrounding bone, resulting in jaw cys

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00282.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

This study is the first to define the expression of hsp 27 in the pulp of the adult human third molar. Using a monoclonal antibody against human hsp 27, immuno‐reactivity was demonstrated in the odomoblasts. odontoblast processes, pulp fi‐broblasts, and smooth muscle and endothelial cells of vessel walls. Nerves were negative. Pulp fibroblasts were characterized by cytoplasraic staining and variable nuclear staining. Odontoblasts also displayed consistent cytoplasmic staining and variable nuclear staining. Western. Northern, and RT‐PCR analysis confirmed the expression of hsp 27 mRNA and protein. Hsp 27 was also shown to be present in both the unphosphorylated and phosphorylated isoforms. In general, nuclear localization and phosphorylation of hsp 27 has been correlated with cells responding to stress or other stimuli. This study demonstrates that pulp from a single human third molar provides sufficient material to support a detailed molecular analysis of gene expre

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00283.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

The aim of the present study was to investigate the sequential expression of placenta! glutathione S‐transferase (GST‐P) during 7, l2‐dimethylbenz[a]anthra‐cene (DMBA)‐induced hamster buccal pouch squamous cell carcinogenesis. Both immunohistochemical and immunoblot analyses were employed to detect the epithelial GST‐P in bamster buccal pouch mucosa over a 15‐week treatment regimen. No GST‐P positivity was demonstrated in the pouches of the control group. GST‐P positive cells were first noted as early as 1 week after DMBA applications. A gradual increase in both the mean number and size of GST‐P‐posi live foci was noted in the first 12 experimental weeks, but a plateau level was approached thereafter. The early GST‐P‐positive‐area were located in the basal layer, or occasionally in the middle layer, of DMBA‐treated hamster buccal pouch mucosa. Later, the stained sites became enlarged and were scattered randomly in different layers or in the whole thickness of the dysplastic and non‐dysplastic epithelium. The keratin layer was only occasionally involved during the first 12 weeks of DMBA treatment but positive staining was more noticeable in the final stage of the experiment. Both exophytic (8–12 weeks) and invasive (13–15 weeks) squamous cell carcinomas showed GST‐P positivity. in both cytoplasmic and nuclear components. Immunoblot analysis revealed no band in the crude tissue extracts of the control pouches whereas GST‐P polypeptide of molecular weight approximately 26 kD was demonstrated in DMBA‐treated pouches over the whole 15‐week treatment regimen. Results of the present work indicate that GST‐P is a stable and persistent label for almost all of the carcinogen‐altered cells during DMBA‐induced hamster buccal pouch carcinogenesis. Immunohistochemically detectable GST‐P may be a poten

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00284.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Verruciform xanthoma (VX) is a rare, benign lesion, mainly found in the oral mucosa. Histologically and ultrastructurally. the lesion is characteristic and well defined. However, the etiology of the lesion remains unclear. The purpose of the present study was to elaborate upon the pathogenesis of VX by evaluation of an additional series of oral examples for human papillomaviruses (HPV). using immunohistochemistry and in situ hybridization, and to further characterize the cellular components of VX immunohistochemically. Twelve specimens diagnosed as VX were retrospectively collected. One of the twelve specimens was positive for HPV types 6/11 by in situ hybridization. None of the twelve specimens demonstrated the presence of HPV antigen by immunohistochemistry. By immunohis‐tochemical studies, the predominant cells in the inflammatory infiltrate were T cells. The foam cells were of monocyte/macrophage lineage. S‐100‐positive (Langerhans) cells were occasionally found in the suprabasal layer of the epithelium. HLA‐DR‐positive keratinocytes were noted at the intense inflammatory sites. Taken together, these findings suggest that an immune response may play a role, at leas! in part, in VX pat

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00285.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

We present an unusual case of glandular odontogenic cyst (GOC) enclosing the crown of an impacted canine that developed in the anterior mandible in a 54–year‐old woman. Microscopically, it contained numerous glandular structures and hyaline bodies in the epithelial lining. The present rare case is sufficiently distinctive to be considered a dentigerous variant of

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00286.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

A desmoplastie variant of ameloblastoma with osteoplasia in the stroma is reported. This tumour presented in the canine/premolar region of the left maxilla of a 31 ‐year‐old woman. It was treated by partial hemimaxiilectorny and immediate reconstruction with a non‐vascularised iliac graft. The location of this lesion, its histology and radiological features differ from those of the conventional ameloblastoma. The behaviour and prognosis of the desmoplastie ameloblastoma (DA) cannot at this stage be predicted due to the small number of cases that have been reported and a lack of long‐term follow‐up. To our knowledge this is the first documentation of the CT and MRI features of desmoplastie ameloblastoma with pathologic co

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00287.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY