摘要:

The etiology of secondary retention is not well understood, but ankylosis is often considered to be an important factor in primary molars. Data concerning the mechanism of secondary retention in the permanent dentition are insufficient, although a possible role of ankylosis has been suggested. In order to analyze the frequency of occurrence and localization of ankylosis in secondarily retained permanent teeth, 26 secondarily retained molars and for comparison six normal molars were studied histologically. These data were compared with the clinical and radiographic findings. Areas of ankylosis were observed along the roots of all secondarily retained molars. In 81% of the cases these areas were located at the bifurcation and interradicular root surface. No signs of ankylosis were found in normal molars. When these results were compared with the clinical and radiographic data, it was obvious that the latter gave many false negative results, because the areas of ankylosis were often too small to be detected clinically or radiographically.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1989.tb01338.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The prognostic value of histopathologic grading of oral squamous cell carcinomas (SCC) has varied from not any to highly significant. We have retrospectively studied all (130) SCCs registered in Norway 1963‐72 in the buccal and maxillary alveolar mucosa. From 68 of these cases biopsy specimens of accetable quality were obtained. Broders’method of grading was compared with a modification of a recent malignancy grading system recommended by Annerothet al.which was performed only within the histologically most invasive areas of the tumors. Cox's multivariate survival analyses showed that this grading in the invasive sites had highly significant prognostic value. Brodersgrade had no prognostic value. The stage of tumor had also prognostic value. These highly significant results indicate that the histologically invasive areas may be primarily responsible for the clinical behavior of the tumor, and this may be of importance for the choice of therapy for oral

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1989.tb01339.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

In order to establish a useful and objective marker of malignancy of oral mucosa, the binding sites forUlex europaeusagglutinin I (UEA‐I),Bandeiraea simplicifoliaagglutinin I (BSA‐I) and peanut agglutinin (PNA) were comparatively examined in the surgical materials from the normal, dysplastic and cancerous epithelium of the oral mucosa by a novel lectin‐antilectin immunoperoxidase method. Based on the staining patterns of the normal keratinized epithelium, UEA‐I was regarded as the marker for the prickle cells, BSA‐I for the cells in the upper prickle to the horny layers, and PNA for those in the basal layer. As the degree of dysplasia advanced, all layers of epithelium came to react with UEA‐I and PNA, whereas the BSA‐I binding was negative. Positive reactions for UEA‐I and PNA were seen in most carcinoma cells other than the keratinizing foci stained by BSA‐I. The results indicate that a UEA‐I‐positive reaction in the basal cells, a PNA‐positive in the prickle cells and loss of receptor for BSA‐I occur in the course of malignant transformation of oral mucosa, and that these lectins may be regarded as useful markers of oral epithelial cy

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1989.tb01340.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The distribution pattern of certain monosaccharides in the epithelial cells of the hamster buccal pouch was studied during carcinoma development induced by 9,10–dimethyl‐l,2–benzathrancene (Dmba). An avidin‐biotin‐peroxidase complex (ABC) immunohistochemical technique with high affinity biotinylated lectins was employed to identify monosaccharides. Lectins used in this experiment included Concanavalin A (Con A), for identifying mannose or glucose, Ricinus communis agglutinin I(RCA‐I), for identifying galactose, and Ulex europaeus agglutinin I(UEA‐I), for identifying fucose. The results show that in normal buccal pouch epithelial cells, fucose or galactose were concentrated predominantly on the cellular membrane, while mannose and glucose were distributed in the cytoplasm. In the epithelial cells undergoing neoplastic transformation induced by Dmba, most cells showed decreased staining of the above‐mentioned monosaccharides, while in other areas the cells were heavily stained. However, the most striking change which occurred was that galactose and fucose shifted from the cellular membrane to the intracytoplasmic area during the malignant transformation. Thus, the changes of anatomic location and intensity of staining of monosaccharides in the buccal pouch epithelium may be used as a criteria for early histochemical diagnosis of malignant

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1989.tb01341.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Cultured baby Syrian hamster kidney cells (BHK 21), synchronized with hydroxyurea (HU), were treated with varying amounts of the carcinogen diethylstilbestrol (DBS) during the first mitosis. DES‐induced morphologic transformation was assessed by fluorescence microscopy of acridine orange, supravitally stained cells. Traditional karyotyping techniques were used to monitor DES‐induced aneuploidy in parallel cell cultures. A total of 618 photomicrographs were obtained by systematic manual scanning of three replicate experiments each using three different DBS treatment levels and a no DES control. These photomicrographs were randomized and graded blind by three independent observers. Each photomicrograph was graded for cellular morphologic transformation and a judgement as to whether DES treatment had been used. A definite dose‐response relationship for both morphologic transformation, as well as for aneuploidy was observed. These findings portend a significance for use of fluorescence microscopy of morphologic transformation of unfixed supravitally stained mammalian cells, for the rapid assessment (24 h or less) of compounds that promote aneuploidization and carcinoge

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1989.tb01342.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Sjögren's syndrome (SS) is an autoimmune exocrinopathy. The salivary glands are the site of activated T‐ and B‐lymphocytes, along with gradual parenchymal destruction, diminished flow and altered composition of the secretory product. At present, Sialochemistry has achieved no significance for the evaluation of SS patient. However, the number of sialochemical publications is steadily growing. This study review current sialochemical findings in patients with SS and relate the observations to the present concept of diagnosis, pathogenesis and prognosis of SS. An ideal combination of the collection of low‐stimulated pure secretion, measurements of absolute flow‐rates, and biopsy from the same glands seem to be unobtainable in SS patients. But two procedures may be appropriate: stimulated parotid secretion combined with parotid biopsies, or absorbance of low‐stimulated labila saliva combined with labial gland biopsy. Sufficient data on disease‐specific alterations in salivary composition in SS are still lacking. However, detection of specific changes in protein synthesis or in glycosylation as well as the detection of inflammatory cell products should be possible with the use of sensitive bioche

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1989.tb01343.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Parotid and submandibular gland function were evaluated in 12 HIV‐1 antibody‐positive men at two visits separated by a median interval of 14.5 months (range 6–22 months). Unstimulated and stimulated flow rates, and the concentrations of total protein, lysozyme, albumin and lactoferrin in these secretions, were determined. Parotid and submandibular gland secretions changed in a specific fashion with time. Lysozyme levels in both glandular stimulated secretions showed significant changes (∼40% and 70% elevated, between visits, in parotid and submandibular saliva, respectively). In addition, the frequency with which albumin was detected in unstimulated parotid secretions increased with time. These findings support earlier results suggesting the presence of alterations in major salivary gland function following HIV‐1 infection. Submandibular gland function appears to manifest these alterations earlier, but with time the parotid secretions show simila

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1989.tb01344.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Oral hyperpigmentation has been observed in six HIV‐infected patients, in two of whom systemic medication (ketokonazole, clofazimine) was supposed to be etiologically involved. Histologically, pigment was found in epithelial basal cells and particularly in subepithelial connective tissue. Ultrastructurally, the presence of premature melanosomes in subepithelial keratinocytes was of interest. Stimulation of melanocytes during HIV infection may occur in association with immunopathologic changes in the oral mucos

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1989.tb01345.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

The occurrence of central papillary atrophy of the tongue among tobacco users, its clinical characteristics and the long term behavior in relation to changes in tobacco use was studied in 182 individuals in Ernakulam district, Kerala, India. Almost all (98%) lesions occurred among bidi smokers. Clinically, about 31% occurred in combination with bidi smoking associated lesions such as palatal erythema (14%), leukoplakia (8%) or both (3%). Histologic evaluation in 12 biopsies using single PAS stained sections showed candidal hyphae in 67%. A 10–yr follow‐up (mean: 6.7 yr) of the 182 lesions showed that the regression was highest (87%) among those who stopped their smoking habit and persistence among those who did not reduce or stop their smoking habits. The findings from this study confirm a strong link between bidi smoking and central papillary atrophy of the tongue in rural Indian populati

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1989.tb01346.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

Cultures of proliferating epithelial cells were established from explants of normal human oral epithelium from healthy young volunteers. The epithelial cells were found permissive for herpes simplex virus type 1 and type 2, coxsackie virus A‐4 and A‐16, adenovirus type 5, measles vaccine, rubella and influenza type A virus‐. Medium from DEAE‐pretreated epithelial cultures infected with two subtypes of human immunodeficiency virus‐1 showed an increasing content of virusprotein with time by antigen ELISA testing. In contrast there was no evidence of infection with coxsackie virus type B‐2, cytomegalovirus, Epstein‐Barr virus and varicella zoster virus. Treatment of the epithelial cells with a non‐cytotoxic dose of cancer chemotherapeutic prior to or after infection with coxsackie virus A‐4 or herpes simplex virus type 1 influenced the virus production dependent on both compound, mode of application, and virus. Adriamycin (doxorubicin) in low dose was found to stimulate the production o

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1989.tb01347.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY