摘要:

Specific mRNA and protein for two major keratins, K14 and K19, were investigated in normal, dysplastic and malignant oral epithelia by combinedin situhybridization and immunohistochemistry. In normal epithelia. K14 mRNA and protein were present almost exclusively in the basal layer of non‐cornified, and in rete‐processes of cornified, sites. Dysplastic epithelium showed irregular extension of the K14 transcript and protein into superficial cells. In squamous cell carcinoma (SCC), K14 transcript was abundant in most samples whilst in one poorly differentiated carcinoma mRNA but no protein was detected. K19 mRNA and its protein were present predominantly in basal cells of noncornified epithelium, whereas in cornified epithelium only mRNA was detected. In dysplasias, K19 transcript was detected in all specimens but its protein was absent in most cases. Even more variations of K19 expression were observed in SSC. These findings indicate differences in the control of expression of K14 and K19 in normal epithelia and show that regulation is further disturbed during dysplastic change and maligna

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00265.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

The frequencies of mutations in the adenomatous polyposis coli (APC). p53, and p16 (MTS1: multiple tumor suppressor 1/CDK4I: cyclin‐dependent kinase 4 inhibitor) tumor suppressor genes were investigated in 23 oral squamous cell carcinomas (SCCs). Loss of heterozygosity (LOH) at the retinoblastoma (Rb) gene locus and on chromosomes 3p (VHL; von Hippel‐Lindau disease tumor suppressor gene locus). 5q (APC) and 9p (p16). and H‐rasoncogene mutations were also studied in the same samples. Techniques employed were polymerase chain reaction single‐strand conformation polymorphism (PCR‐SSCP), DNA sequencing and PCR‐microsatellite analyses. Mutations of the p53 gene were detected in 26% (6/23) of the tumor specimens. APC and p!6 were not mutated in any of the 23 oral SCCs studied. LOH was detected in 17% (2/12 informative cases) at the Rb, in 33% (4/12) on 3p, in 17% (4/23) on 5q and in 30% (3/10) on 9p. Mutations of the H‐rasgene were detected in 9% (2/23). The only correlation between these genetic alterations and clinicopathologic characteristics was that mutations of the p53 gene were detected more frequently in oral SCCs with lymph node metastasis than in those without it (P<0.05). These results demonstrate that mutations of the p53 gene and LOH on 3p and 9p frequently occur in oral SCC and play important roles in the development and/or progression of this comm

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00266.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

The carriage of fiveCandidaspecies in the mouths of normal and siaioadenectomised rats was determined for periods up to 30 days after inoculation into the oral cavity. In both test and control animals.Candida albicanswas the species recovered in greatest quantities at all periods, followed byC. parapsilosisandC. tropicalis.In contrast.C. gullliermondiiandC. kruseiwere isolatable only in small numbers and only from the 1st up to the 5th day; they were not present thereafter. Sialoadenectomy favoured oral colonisation only byC. albicans(P<0.05) and did not influence the carriage of the other species.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00267.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

This is a descriptive study of patients with symptomatic lichenoid‐like reactions of the lips in contact with composite restorations (n=12). Clinically, the lesions appeared as symptomatic single or multiple erythematous areas that included white papules.Candidawas found by cultivation or histopathological examination in 7 of the lesions. Patch testing (n=7) did not reveal any reactions to composite components, except for one patient who showed a delayed hypersensitivity reaction to formaldehyde. Replacement of existing dental materials parallel to antifungal treatment resulted in healing or significant improvement in 7 of 9 patients. This study shows that resin components can be associated with adverse reactions in the oral mucosa of the lips and that these reactions may be accompanied by an infection withCandid

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00268.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

To facilitate recognition of the oral mucosal lesion that develops in rats with graft‐versus‐host disease (GVHD) induced by injecting spleen cells of parental strain rats (Brown Norway) into non‐irradiated (Brown Nonvay×Lewis) F1 hybrid rats, we followed the development of the tongue lesion histologically and immunohistochemically. This assessment revealed an increase in the number of MHC class II+cells with dendritic shape in the lamina propria to be the earliest stage of the tongue lesions in GVHD rats. The subsequent mononuclear cell infiltration with epithelial cell destruction, characteristic of GVHD. consisted of CD8+cells and macrophages. Our findings seem to indicate that MHC class II+cells with dendritic shape may provide antigen presentation in the induction of local immunological responses, including tissue destruction, by CD8+cells and macrophages in the tongue of GVH

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00269.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

E‐selectin is an adhesion molecule, expressed by cytokine‐activated endothelial cells, that participates in the binding of neutrophils. Recent studies in our laboratory documented binding of the E‐selectin‐specific monoclonal antibody H4/18 to keratinocytes in inflamed human oral mucosa. particularly gingival epithelium. To determine whether this immunoreactivity was due to expression of authentic E‐selectin, the presence of E‐selectin mRNA in gingival epithelium was analysed using the polymerase chain reaction (PCR). Reverse transcription of epithelial RNA and amplification of cDNA with E‐selectin‐specific primers resulted in the formation of a 178 nucleotide PCR product identical to that obtained from cytokine‐activated endothelial cells. Sequencing of the PCR product revealed 100% homology between epithelial and endothelial E‐selectin fragments. Epithelial preparations did not contain mRNA for von Willebrand factor, excluding the possibility of contamination by endothelial cells. These results confirm immunohistochemical studies of E‐selectin immunoreactivity in human oral mucosa and demonstrate that E‐selectin expression is not

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00270.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

In consideration of the close relationship between radicular cysts and alveolar bone, it is important to evaluate the potential involvement of the neighbouring bone tissue in such lesions. In the present study, using cytochemical, immunocytochemical and morphological analyses, presumptive osteoclast precursors were revealed in the connective tissue of radicular cyst capsules. The osteoclastic nature of these cells was postulated by their positive staining for the enzyme tartrate‐resistant acid phosphatase (TRAP) and by the expression of vitronectin receptor (VnR) on their cell surface. However, these cells did not express the vacuolar‐type proton pump, suggesting that they may represent early osteoclast precursors infiltrating the cyst capsule. Cysts also contained activated small blood vessels whose endothelial cells expressed the VnR. This integrin receptor is important in the adhesion of preosteoclasts to the endothelial lining, a necessary step for their emigration out of the vasculature. Therefore, the intracystic vessels could represent a substrate for preosteoclast recruitment. These precursor cells may then reach the perialveolar bone surface and contribute to bone demolition together with those recruited by the resorbing surfaces. The bone‐destroying potential of radicular cysts was confirmed by the presence of numerous osteoclasts with large resorption areas on the perialveolar bone surfaces exposed to the cyst capsules. The resorbed surfaces were usually located around the vascular canals of the Haversian sy

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00271.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Immunoglobulin (Ig) kappa (κ) and lambda (λ) light chain mRNA‐expressing cells were investigated byin situhybridization (ISH) to assess the local humoral immune response in human dental periapical lesions. Twenty‐seven biopsy samples (17 periapical granulomas and 10 radicular cysts) were examined. Both types of light chain mRNA‐positive cells were detected in formalin‐fixed/paraffin‐embedded tissue sections in all samples. Plasma cells showed weak to strong cytoplasmic staining with both probes and background staining was negligible. The ISH methodology is specific and sensitive in detecting Ig light chain mRNAs and retains cell morphology well. κ to λ ratios showed moderate variability for both granulomas and cysts (mean=1.66±0.85 SD. 1.47±0.51, respectively). There was no significant difference in light chain distribution between granulomas and cysts. Ig κ and λ light chain proteins were also studied by immunohistochemistry (IHC) but the results were disappointing due to excessive background staining. This study confirms that Ig is locally produced in periapical lesions and that the ISH method localises Ig light chain‐containing cells better than IHC. The wide variability in κ/λ ratio may support the concept of non‐specific multibacterial inf

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00272.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Sialadenoma papilliferum (SP) is a rare, benign, salivary gland tumor which most commonly arises in the palate. It has a typical biphasic gross and microscopic appearance which distinguishes this tumor from other papillary‐like tumors of the oral cavity. This study reports the clinico‐pathologic features of 5 new cases and analyzes the morphologic and immunophenotypic features of their cell components. Adluminal epithelial cells of duct‐like structures appeared immunoreactive to cytokeratin 19 and to S‐100 protein antibodies; two subsets of basally‐located cells were identified by means of immunohistochemistry. One cell subset expressed cytokeratin 14, S‐100 protein. GFAP, vimentin and smooth muscle actin immunoreactivity: this antigenic profile is consistent with myoepithelial differentiation. The second subset of basal cells expressed eytokeratins 13 and 14 reactivity' but it was negative to all other antibodies. Anti‐CD la and anti‐S‐100 protein antibodies revealed distinct cells with dendritic processes which resembled Langerhans cells. The extralobular location of SP, the continuity between neoplastic duct‐like structures and the surface epithelium along with the presence, within the excretory ducts adjacent to the tumour, of lesions which possibly precede the development of SP give further strength to the hypothesis of an origin from the excretory ducts of this tumor. Langerhans cells seem to be present in Sialadenoma papilliferum but their role in this tum

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00273.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Reports on dental abnormalities in connection with hypophosphatasia almost exclusively describe changes in primary teeth. A 23‐year‐old man with hypophosphatasia, first diagnosed at the age of 8 months, is described; histologically and radiographically verified signs of the condition were present in the permanent dentition. The findings included a reduced level of the marginal alveolar bone supporting the upper central incisors, which had to be extracted. The molars displayed large coronal pulp chambers. Histologically, the upper incisors demonstrated abnormal root cementum, with areas of dentin resorption, as well as disturbances of the mineralization of the coronal dentin. The patient also had signs of abnormal root resorption of molars. The potential involvement of permanent teeth puts children with hypophosphatasia at risk of developing oral complications during adolescent and adult l

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb00274.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY