摘要:

Immunohistochemical investigation of PCNA and Ki‐67, two diverse nuclear proteins essential to the cell cycle, was undertaken in archival, formalin‐fixed and paraffin‐embedded specimens of giant cell fibroma (GCF) and peripheral giant cell granuloma (PGCG). GCF multinucleated cell nuclei were mostly PCNA+, although there was variability in staining intensity. This indicates heterogeneity in nuclear PCNA metabolism of GCF multinucleated cells, and it is possible that the most intensely stained nuclei have passed through the cell cycle more recently compared to the less immunoreactive nuclei. However, the absence of Ki‐67 immunoreactivity in GCF multinucleated cells, and absence of mitoses in GCF multinucleated cells, suggests that cell cycling in the absence of cytokinesis is not involved in GCF multinucleated cell formation. Alternatively, GCF multinucleated cells possibly form by fusion of mononuclear cells previously identified as fibroblasts, although this theory cannot be confirmed by the data presented in this study, and the histogenesis of GCF multinucleated cells remains unclear. In contrast, absence of either PCNA or Ki‐67 immunoreactivity in PGCG multinucleated cells is consistent with an osteoclast lineage and formation from differentiated mononucl

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb01371.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

106 human TMJ specimens from 53 individuals (age: 65–85 years) were examined macro‐ and microscopically and measured for discal and condylar cartilage thickness at five points defined on a mediolateral axis along the middle dense pan of the disc. 27% of the discs showed lateral and laterocentral perforations. In 8% the lateral portion was thinned down to a translucent layer. 62% showed no gross destruction or deviation in shape and thickness. The remaining 3% displayed extensive destruction and their condyles were osteoarthrotic. All discs showed a significant decrease in thickness from their medial towards their lateral portions. The condyles displayed either a smooth articular surface (16%) or an irregular and progressively remodelled surface (81%) with histologically normal fibrocartilage. The condylar cartilage did not show a significant gradient of thickness in amediolateral direction. The results support our previously developed working hypothesis, that the joint is seemingly loaded along its entire articular surface and that a lateral disc perforation in older individuals can be due to a physiological process of wearing rather than to a pathological sequel of functional disord

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb01372.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

A follow‐up study was carried out to evaluate the prognostic value of hairy leukoplakia (HL) and oral candidosis (OC) in a cohort of 111 asymptomatic Mexican HIV infected patients. Oral exams were performed at baseline and every 6 months, from September 1989 to March 1994. Chi‐square contingency table test, the Kruskall‐Wallis one‐way analysis of variance, the Kaplan‐Meier product‐limit method and the log rank test were used for the analysis. Univariate and multivariate Cox's proportional hazards analysis were also performed. Fifty‐four patients (51%) progressed to AIDS (initially 36 CDC‐II and 18 CDC‐III). Individuals with HL and/or OC, showed faster development to AIDS than subjects without lesions or other HIV‐related manifestations (P=0.008). The presence of OC, HL or both always remained significant despite adjustment for total lymphocytes, CDC stage, zidovudine therapy or its combinations. Oral lesions in HIV infection may be regarded with other clinical and laboratory studies as markers of HIV disease progression and as indicators to begin antir

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb01373.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

In a preliminary study on anti‐human cytomegalovirus (HCMV) antibody (Ab) by ELISA. the serum anti‐HCMV/IgG Ab concentrations in 22 patients with recurrent aphthous ulcers (RAU) in the remission stage were found to be significantly higher than in 22 control subjects (121±42 vs 100±27, P<0.05) and in 39 patients with RAU in the active stage (121±42 vs 88±45, P<0.01). Therefore, the potential of HCMV as an etiologic agent in RAU was proposed and studies using the polymerase chain reaction (PCR) andin situhybridization (ISH) have been performed to investigate the possible presence of HCMV DNA in pre‐ulcerative oral aphthous lesions in patients with RAU or Behçet's disease (BD) of the mucocutaneous type. For this purpose, formalin‐fixed biopsy specimens were obtained from 13 pre‐ulcerative oral aphthous lesions, 2 samples of normal oral mucosa and 1 ileal mucosal lesion from 9 RAU patients and 4 BD patients. Five specimens of normal oral mucosa from 5 normal control subjects and 12 specimens of oral erosive or ulcerative lesions from 12 patients with erosive lichen planus (ELP) were also included. By PCR, HCMV DNA was detected in 5 of the 13 (38.5%) pre‐ulcerative oral aphthous lesions. 3 from RAU patients and 2 from BD patients. The ileal mucosa specimen was also HCMV DNA‐positive, whereas HCMV DNA was not demonstrated in any of the 7 specimens of normal oral mucosa from RAU patients and normal control subjects; 12 specimens of oral lesions from ELP patients were similarly negative. ISH did not detect HCMV DNA in any of the biopsy specimens from RAU patients and control subjects. Our findings suggest that HCMV may be an etiologic agent in some c

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb01374.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Oral lichen planus (OLP) is a T cell‐mediated inflammatory disease of the oral mucosa. T lymphocytes accumulate within OLP lesions by extravasation from the local microvasculature and subsequent migration to the oral epithelium. Tumor necrosis factor‐alpha (TNF‐α) is a cytokine involved primarily in T cell‐mediated immunopathological reactions, and it is implicated in diseases which bear clinical and histological similarities to OLP. This review examines the role of TNF‐α in the initiation and progression of OLP, and summarises evidence for a key role for TNF‐α in this disease. A unifying hypothesis for the involvement of TNF‐α in the immunopathogenesis of OLP is presented. Based on this model, a variety of current therapies are explained and several alternative app

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb01375.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Expression of mRNA for IL–α, IL‐1β, IL‐2, IL‐4, IL‐5, IL‐6 and TNF‐α in inflamed gingiva was quantitatively examined by ribonuclease protection assay andin situhybridization. The IL‐1β mRNA expression level was statistically high (P<0.05) in periodontitis‐affected tissues compared with that in gingivitis‐affected tissues. The densities of macrophages (identified as CD68‐positive cells) and CD45RO‐positive cells infiltrating in the inflamed gingiva correlated statistically with IL‐1β transcript levels (macrophages. P<0.001: CD45RO‐positive cells, P<0.002).In situhybridization revealed IL‐1β mRNA expression in infiltrating cells, presumed to be macrophages. The IL‐1α and IL‐6 mRNA expression levels were much lower than the IL‐1β transcript level, and mRNAs for IL‐2, IL‐4, IL‐5 and TNF‐α were negligible in these gingival tissues. The results indicate that IL‐1β is a cytokine expressed predominantly in inflamed gingiva and reflects the d

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb01376.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Exons 1–3 of the p16/CDKN2 gene, exons 4–9 of the p53 gene and exons 1 and 2 of H‐, K‐ and N‐rasgenes were screened for mutations by a combination of immunohistochemistry and single‐strand conformational polymorphism (SSCP) analyses of polymerase chain reaction products from human surgical samples of both frank oral squamous cell carcinomas and premalignant lesions. The samples included 20 squamous cell carcinomas. 10 epithelial dysplasias and 10 epithelial hyperplasias. No identifiable gene mutations were detected in any of the dysplasias or hyperplasias, while 2 (10%) deletions and 2 (10%) mutations of p16/CDKN2, along with 5 (25%) p53 mutations were found in the advanced carcinomas, yielding characteristic p16/CDKN2 and p53 changes. A mutation in the K‐rasgene was found in single carcinoma and dysplastic samples. From the data, it can be argued that p16/CDKN2 and p53 mutations are relatively late occurrences in human oral tumorigenesis and that genetic alterations of therasgenes may not play a significant role in squam

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb01377.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Clinical (n=281) and histopathological (n=141) characteristics of toombak‐associated oral mucosal lesions detected in an epidemiological study in northern Sudan in 1992/93 are described. The lesional site in the majority of toombak users was the anterior lower labial groove and the lower labial mucosa. 4 degrees (1–4) of clinical severity of lesions, similar to those used to characterise Swedish snuff‐dipper's lesion, were applied. An association between the severity of mucosal lesions and a longer lifetime duration (>10 years) of toombak use was found, but the severity was not related to the daily frequency of the habit. Parakeratosis, pale surface staining of the epithelium and basal cell hyperplasia were commonly observed, but epithelial dysplasia was infrequent (10/141). The most significant observation was a PAS‐positive amorphous deposit between the lamina propria and the submucosa, found in 25/141 biopsies. The clinical and histopathological features of toombak lesions are closely similar to Swedish moist snuff‐dipper's lesions and this may reflect the high alkalinity of these products, resulting in an alkaline burn on the oral mucosa following chronic exposure. The low prevalence of epithelial dysplasia implies a low risk of malignant transformation. Nevertheless, the high concentrations of tobacco‐specific nitrosamines present in toombak, and the high prevalence of oral cancer in Sudan, mandate biopsy and careful histopathological analysis of any such lesions detected

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb01378.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

Forty oral cancer patients identified consecutively in Changhua Christian Hospital between 1990 and 1992 were compared with 160 population‐based controls, matched for sex, age, area of residence, and educational background. Betel quid chewing was positively associated with the risk of oral cancer with an adjusted odds ratio of 58.4 (95% CI: 7.6–447.6). The greater the number of years of chewing betel quid, the higher the risk of oral cancer; the adjusted odds rations were 12.9, 93.7 and 397.5 for40 years of betel chewing as compared with the non‐users. The risk also increased with the quantity chewed per day; the odds ratios for those chewing20 quids/day were 26.4, 51.2 and 275.6, respectively. These odds ratio estimates were all statistically significantly different from the null value

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb01379.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY

摘要:

The expression of p53 protein was studied in odontogenic keratocysts (OKC, 11 solitary, 5 recurrent and 6 NBCCS cysts), radicular (RC,n=5) and dentigerous (DC,n=5) cysts, using a panel of antibodies to p53 (clone BP53‐12, clone 1801 and polyclonal CM1) and a sensitive biotin‐streptavidin method on paraffin embedded sections. Of the three antibodies tested, clone BP53‐12 gave the most intense and consistent nuclear staining pattern. Clone 1801 and polyclonal CM1 stained only 38% and 71% OKC linings, respectively, but not RC and DC linings. However, BP53‐12+cells were detected in the epithelial linings of all cyst types. Quantification of BP53‐12+cells was performed by manual counting and by relating cell number to unit length of basement membrane as determined by TV image analysis. BP53‐12+cell counts in solitary OKC linings (25.5 ± 11.0 cells/mmBM) were significantly greater than those in DC (9.3 ± 4.9 cells/mmBM, P<0.01) and RC (6.7 ±2.6 cells/mmmBM. P<0.01) linings. The epithelial distribution of positive cells in OKC was predominantly suprabasal, which also varied from that of DC and RC linings (P0.1). When data for the NBCCS‐related OKC group were excluded, there was a significant correlation (r=0.55. P<0.01) between p53 and Ki67 labelling. To detect the presence of p53 gene mutations, genomic DNA, extracted from paraffin sections of OKC (4 solitary, 2 recurrent and 4 NBCCS cysts). RC (n=3) and normal oral mucosa (n=1), was subjected to a combination of polymerase chain reaction and single‐stranded conformation polymorphism (PCR‐SSCP) analysis for exons 5‐10 of the p53 gene. Exon 4 was not analysed because of compromised DNA quality. No abnormality in banding patterns was found and all samples save results similar to DNA from known, sequenced, normal p53 gene controls. Absence of p53 mutations within exons 5–9 was confirmed by the direct sequencing of 2 fresh frozen OKC samples (1 solitary and 1 NBCCS cyst). These results suggest that over expression of p53 protein in OKC epithelium, detected by immunocytochemistry, is not reflected by alteration of the p53 gene and presumably reflects overproduction and/or stabili

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1996.tb01380.x

出版商:Blackwell Publishing Ltd    

年代:1996

数据来源: WILEY