摘要:

Abstract.Ultrastructural studies have been carried out on epithelium taken from the oral lesions of tylosis‐related leukoplakia and preleukoplakia in a group of patients known to be at high risk for esophageal carcinoma. Several structural abnormalities are described; in particular, the presence of intranuclear electron dense particles 90 nm in diameter. Similar particles have been found in the nuclei of epithelial cells from an esophageal carcinoma developing in one of the patients under stud

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1975.tb01852.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Abstract:The keratohyalin granules from 25 human oral leukoplakias, showing benign hyperorthokeratosis histologically, were examined employing a series of histochemical techniques. The tissues were fixed in 10% neutral buffered formalin, 80% methanol, or Carnoy's fluid. The keratohyalin granules stained intensely with Pauly's reagent, Congo red and Harris hematoxylin, indicating the presence of proteins. This was confirmed by abolishing the staining reaction by pretreatment with proteolytic enzymes. The keratohyalin granules also reacted with methyl green‐pyronin by staining pink at their peripheries; this staining was abolished by pretreatment with ribonuclease, indicating the presence of ribo‐nucleotides. The keratohyalin granules partially stained with toluidine blue and colloidal iron, indicating the presence of acidic polysaccharides. The keratohyalin granules did not react with the Feulgen reagent, suggesting the absence of DNA. Our studies indicate that the keratohyalin granules in human oral leukoplakia are primarily protein(s) complexed with polyribonucleotides. The presence of a carbohydrate moiety suggests the possibility of a protein‐polysaccharide component in the gra

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1975.tb01853.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Abstract:The pathology of the fibrous epulis in the dog is described and found to be comparable to that seen in humans. Emphasis is placed on the apparent relationship between the hyperplasia of the covering epithelium and calcification of the connective tissue component, and a theory to explain this relationship is proposed.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1975.tb01854.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Abstract.Human supragingival dental plaque was collected from patients with various degrees of caries and periodontal disease. Plaque extracts, prepared in five different solutions (four varied from pH 1.8 to 12.7; one contained urea), were analyzed by polyacrylamide gel electrophoresis, and tested for amylase and lysozyme enzyme activity. Because no qualitative or quantitative advantages of using the extremes of pH or urea were observed, all subsequent extracts were prepared in phosphate buffered saline at pH 7.3. Concentrated extracts were fractionated by gel filtration and characterized by polyacrylamide gel electrophoresis, peptide mapping, molecular weight estimation, determination of enzymatic activities and amino acid and carbohydrate analyses. Regions of similarity among the gels were revealed by comparing the electrophoretic patterns of pooled plaque extract, normal serum and whole saliva. The elution pattern of pooled plaque extract from a standardized Sephadex G‐200 column indicated the presence of both high and low molecular weight proteins that might have correlated with the components of normal serum and saliva. A predominant and dialyzable third fraction had no correlate in either serum or saliva. The small peptides in this fraction were subjected to amino acid, carbohydrate and peptide map analyses. The most abundant amino acids were alanine, glutamic acid, glycine, valine, leucine, lysine and serine. These small components contained no neutral or amino sugars. Pooled plaque extract and the small peptides exhibited similar peptide map

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1975.tb01855.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Abstract.From collected supragingival plaque, extracts were prepared for immunochemical analyses. Extracted sediments were examined by fluorescein‐labeled antibodies for the presence of immunoglobulins. Precipitation with monospecific and polyvalent antisera revealed IgA, IgG, secretory component, C3, α2macroglobulin, lactoferrin, and albumin in the extracts. Gel filtration of pooled plaque extract yielded two fractions that contained the aforementioned proteins and a prominent, dialyzable third fraction that was immunochemically nonreactive. IgA, IgG, secretory component, and light chains were shown, by immunofluorescence, to be present in washed, pooled plaque sediment. Release of these immunoglobulins by urea treatment indicated their probable participation in immune complex

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1975.tb01856.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY

摘要:

Abstract.Separate plaque samples (collected from 13 patients who had experienced caries and various degrees of periodontal disease) were each dispersed in 1 ml of distilled water, homogenized and lyophilized. Each lyophilisate was extracted in 1 ml of buffered saline, concentrated and analyzed. Enzyme activity studies revealed amylase in all plaque samples. Lysozyme was present occasionally. By radial immunodiffusion, IgG and IgA were shown to be in plaque extracts of some patients. Immunofluorescence examination of the sediments of individual plaque samples revealed that IgG and IgA occurred frequently. Polyacrylamide gel electrophoresis patterns of the extracts of plaque from different individuals exhibited marked variability despite some zones of similarity. Peptide maps of the dialysable material of the individual plaque extracts were remarkably similar.

ISSN:0904-2512    

DOI:10.1111/j.1600-0714.1975.tb01857.x

出版商:Blackwell Publishing Ltd    

年代:1975

数据来源: WILEY