摘要:

Microcolumn chromatography and a new test tube method for quantitation of hemoglobin A2were compared with column chromatography on DEAE-Sephadex, starch block and cellulose acetate elec-trophoresis to ascertain their relative accuracy, precision, reproducibility and speed. One hundred seventy-four blood specimens, including 90 samples from genetically provenβ-thalassemia hetero-zygotes were examined. The mean Hb A2values in normal andβ-thal-assemia heterozygotes were: 2.3Z and 4.7%, respectively, determined by microcolumn chromatography; 2.3% and 4.9%, respectively, determined by a new test tube method; 2.5% and 4.6%, respectively, determined by column chromatography on DEAE-Sephadex; 2.6% and 4.8%, respectively, determined by starch block electrophoresis; and 2.4% and 4.8%, respectively, determined by cellulose acetate electrophoresis. Although all five methods were found to be reliable and reproducible, the microcolumn chromatographlc method and the newly developed test tube method using DE-52 cellulose are the most rapid, reproducible, economical, and well suited for large scale surveys.By microcolumn chromatography, 7,953 school children and 2,710 other cases were screened for the quantity of Hb A2. In these samples, 578β-thalassemia heterozygotes were detected.

ISSN:0363-0269    

DOI:10.3109/03630267709003911

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

摘要:

The a andβchains of the hemoglobins A, S, Leslie and N-Baltimore have been isolated as PMB derivates by CM-cellulose and DEAE-cellulose chromatography. The relative affinities of theβA,βs,βLeslieandβN-Baltimore chains forαchains were measured through quantitation by chromatography of Che hemoglobins A and Leslie, A and S, and A and N-Baltimore that were formed when variable amounts ofαchains were added to a mixture of equal amounts of the appropriateβchains. The data indicate a greatly decreased affinity ofβLesliechains forachains; a similar preference of a chains forβAchains was observed for mixtures Involvingα,βA, andβschains, but the affinity ofβschains for a chains was higher than that ofβLesliechains. TheβN-Baltimore chains assembled with a chains at a similar rate asβAchains. The data as Interpreted Indicate that the affinity of certainβchains for a chains can be a major post-translatlonal control mechanism which regulates the level of aβchain variant in heterozygotes.

ISSN:0363-0269    

DOI:10.3109/03630267709003912

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267709003913

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267709003914

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267709003915

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267709003916

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267709003917

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor