ISSN:0363-0269    

DOI:10.3109/03630268008996228

出版商:Taylor&Francis    

年代:1980

数据来源: Taylor

摘要:

A newly developed HPLC procedure (1) was used to study the percentages ofGγandAγchains in the Hb F from newborn and patients with sickle cell anemia. The method gives data comparable to those obtained with a more laborious chemical procedure. However, the presence of theAγTchain, i.e., theAγchain with an Ile→Thr substitution at position 75, interferes with the determination of theGγtoAγratio because theaγtandGγchains have the same chromatographic mobility.

ISSN:0363-0269    

DOI:10.3109/03630268008996229

出版商:Taylor&Francis    

年代:1980

数据来源: Taylor

摘要:

Restriction enzyme digestion analysis and direct human globin gene cloning have permitted analysis of the physical arrangement of nucleotide sequences within and surrounding the human globin genes. With these methods it has been shown that the linear arrangement 5′to 3′of the globin genes isGγ-Aγ-δ-β. TheGγandAγgenes are separated by about 3.5 kilobases (kb), while theAγandδgenes are 15 kb apart, and theδandβ6.5 kb apart. Each of these genes contains a large intervening sequence (IVS) of approximately l kb in precisely the same position between codons 104 and 105. In addition, each of these genes has a small IVS between codons 30 and 31.

ISSN:0363-0269    

DOI:10.3109/03630268008996230

出版商:Taylor&Francis    

年代:1980

数据来源: Taylor

摘要:

Gel blot hybridization studies on DNA from individuals heterozygous for different types of hereditary persistence of fetal hemoglobin (HPFH) have shown a good correlation in gene structures with those deduced from data obtained in studies of the hemoglobins found in members of the families. The recent data have also shown that in certain individuals additional strong hybridizing bands are present. The origin of these bands is as present unresolved, but it may be that they represent additional genes.

ISSN:0363-0269    

DOI:10.3109/03630268008996231

出版商:Taylor&Francis    

年代:1980

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630268008996232

出版商:Taylor&Francis    

年代:1980

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630268008996233

出版商:Taylor&Francis    

年代:1980

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630268008996234

出版商:Taylor&Francis    

年代:1980

数据来源: Taylor

摘要:

As an allosteric protein, hemoglobin can assume at least two different quatenary structures, a deoxy or T for tense conformation and an oxy or R for relaxed conformation (2,3) depending upon its state of ligation. Because the pK values of oxy and deoxy- hemoglobins are different, molecules in the R conformation should behave differently than molecules in the T conformation on ion exchange chromatography. Kilmartin et al. (4) designed an anaerobic cation exchange chromatographic procedure with which they separated the deoxy hemoglobin from oxy hemoglobin and other R conformation hemoglobin components like CO-hemoglobin and sulfohemoglobin. In this report we describe the further development of Kilmartin's procedure to separate two electro-phoretically silent hemoglobin mutants, Hb Potomac [β101 Glu→Asp] (5) a high oxygen affinity variant, and Hb M-Milwaukee [β67 Val→Glu] (6,8) a low oxygen affinity mutant from Hb A. The modifications we have made include (a) applying the hemolysate at a partial pressure of oxygen determined from an examination of the oxygen equilibrium curve to produce a favorable ratio of T to R conformations of the two hemoglobns to be separated, (b) controlling the partial pressure of oxygen in the chromatographic developer, and (c) monitoring the oxygen tension of the column effluent during the development of the chromatogram.Hb Potomac. This work has been supported by grants HL20142 and AMl785O from the National Institutes of Health, U.S. Public Health Service.

ISSN:0363-0269    

DOI:10.3109/03630268008996235

出版商:Taylor&Francis    

年代:1980

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630268008996236

出版商:Taylor&Francis    

年代:1980

数据来源: Taylor

摘要:

Separation of Hb chains constitutes an important step in the procedures of (a) the studies on the primary structure of polypeptide chains of hemoglobins, (b) the detection of chain anomaly of the abnormal hemoglobins, and (c) the investigation on the biosyn-thetic ratio of theαchain to the non-αchain.

ISSN:0363-0269    

DOI:10.3109/03630268008996237

出版商:Taylor&Francis    

年代:1980

数据来源: Taylor