摘要:

In an electrophoretic study of 15,661 Jamaican cord bloods, 8 rareβ-chain variants were found in 18 subjects in addition to the commonβ-chain variants, Hb S and Hb C. The heterozygote frequencies for Hb S and Hb C were 10.1% and 3.7% respectively. The most frequent of the rareβ-chain variants were Hb Korle Bu (β73 Asp→Asn) (7 cases) and Hb Osu-Christiansborg (β52 Asp→Asn) (3 cases). One new B-chain variant, Hb Caribbean (8 91 Leu→Arg) was found.

ISSN:0363-0269    

DOI:10.3109/03630267809005351

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

摘要:

We cultured human umbilical cord blood and adult peripheral blood erythropoietic precursors in methylcellulose clonal assay and measured the synthetic rates of HbA, A2, and F. Hb was labeled with14C-amino acid in culture and separated by slab-gel isoelectric focusing and quantitated by autoradiography. While the mean percentage of HbF synthesized by adult cells was only 20.1%, that of umbilical cord blood cells was 53.9%, which corresponds closely to the biosynthetic capabilities of umbilical cord blood reticulocytes. Variations in the erythropoietin concentrations did not influence the percentage of HbF. Erythropoietic cell cultures of human umbilical cord blood may provide an important means for studying the molecular mechanisms controlling physiological Hb switching in the perinatal period.

ISSN:0363-0269    

DOI:10.3109/03630267809005352

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

摘要:

Three→β-thalassemia homozygotes were found in a Mexican family. Both parents and two siblings had heterozygous 63-thalassemia with about 10% Hb F, mild microcytosis and mild hypochromia, while three siblings were normal. Hb F, which was the only Hb component in the homozygotes, had equal quantities of Gγand Aγchains as in GγAγ-→β-thalassemia. The homozygotes had comparable erythrocytic indices which were about the same as those of the heterozygotes. However, two were clinically and hematologically healthy but the third had a severe chronic hemolytic anemia and a more severein vitrochain synthesis imbalance than her homozygous sisters. Comparison of these cases with other GγAγ63-thalassemia homozygotes and with GγAγHPFH homozygotes indicates the possibility that the proliferation of F-cell precursors may be defective in→β-thalassemia.

ISSN:0363-0269    

DOI:10.3109/03630267809005353

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

摘要:

Hemoglobins D-Los Angeles (β121 Gin) and Aida (α64 Asn) were encountered in an Asian Indian, unassociated with any clinical manifestations. These hemoglobins had normal oxygen affinities and were stable to heat and isopropanol. The ratio ofαchain synthesis toβchain synthesis was close to unity. The identical change in isoelectric points of the two variants produced an interesting electrophoretic pattern.The coexistance of more than one hemoglobin variant in an individual or family has provided valuable

ISSN:0363-0269    

DOI:10.3109/03630267809005354

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

摘要:

Hb Hofu,αβ[126 (H4) Val→Glu], was found in 10 members of 2 apparently unrelated Valmiki families in central India. None showed evidence of hemolysis and hemoglobin levels were normal in most. In two individuals, Hb Hofu occurred in combination with Hb S, but neither had clinical manifestations of sickle cell disease. In samples containing Hb Hofu, the isopropanol precipitation test was positive. Quantitation of the hemoglobin fractions by DEAE-cellulose chromatography showed that Hb Hofu constituted a mean of 23-25% of the total whether in combination with Hb A or Hb S.

ISSN:0363-0269    

DOI:10.3109/03630267809005355

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267809005356

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267809005357

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267809005358

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267809005359

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267809005360

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor