摘要:

Hemoglobin A was modified in vitro with 0.02—0.03 M glutarylsalicylamide for two hours at pH 7.2 and 37°C. The extent of modification was about 30—50%, as estimated by visual comparison after electrophoretic separation. A substantial decrease in oxygen affinity of modified hemoglobin solutions was observed. Similar results were also obtained for dilute cell suspensions of washed red blood cells and whole blood after GSM modification. Other properties such as cooperativity, Bohr effect and 2,3-DPG dependence remained essentially unchanged. Although the site(s) of modification have not been determined, it is unlikely that they would involve any amino acid residue contributing to the above allosteric properties.

ISSN:0363-0269    

DOI:10.3109/03630267809074778

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

摘要:

Globin chain synthesis and globin mRNA content were studied in blood cells of a patient homozygous for Hb Lepore. Peripheral blood cells incubated with tritiated leucine synthesized approximately 1.5 to 3% as many Lepore globin chains asαchains. Globin mRNA in peripheral blood cell RNA was assayed by molecular hybridization assays using humanαandβcDNA, and the results indicated the presence of approximately 1% to 2% as muchβ-like mRNA (presumablyδβLepore mRNA) asαmRNA. The amount of Leporeδβchain mRNA in peripheral blood cells is therefore proportional to the amount of Lepore globin chain synthesis in the same cells. An incidental observation was the finding that peripheral blood cell RNA of this patient, at a time when she was being heavily transfused, contained substantially higher levels ofβ-like mRNA (relative to a mRNA) than in subsequent studies. Cell-free translation of this mRNA however revealed that it contained authenticβchain mRNA which must have been derived in some way from the transfused blood cells.

ISSN:0363-0269    

DOI:10.3109/03630267809074779

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

摘要:

A new method for determining the biosynthetic ratio of globin chains in human reticulocytes is described.Washed red cells, enriched in reticulocytes by high speed centrifugation at 30°C, are incubated for 45 min. at 37°C with Minimum Essential Medium (Eagle) and3H-Leucine.Globin is prepared from hemolysate by acid-acetone method. Globin chains are separated by electrophoresis on“Cellogel”at pH 6.3 in 0.04 M sodium phosphate buffer, containing urea 6 M, and stained with 0.5% Coomassie Blue. The strips of cellulose acetate containing the globin chains are dissolved in 10 ml of dioxan-naphtalene scintillation fluid, and counted in a liquid scintillation counter. The biosynthetic ratio of globin chains is calculated from the ratio of the total counts incorporated into theαand non-αchain zones.This method gives results in agreement with those obtained by macro-CMC column chromatography, but it is much simpler and faster. This electrophoretic method appears particularly suitable as a diagnostic tool in a variety of hematological disorders.

ISSN:0363-0269    

DOI:10.3109/03630267809074780

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

摘要:

Hb Vancouver (α2β273 (E17) ASP→TYR) was found in combination withβ°thalassemia trait in a Chinese male who presented with splenomegaly and thalassemia intermedia (3). The family study has revealed two members withβthalassemia trait, one heterozygote for Hb E, and two heterozygotes for Hb Vancouver. The Hb Vancouver heterozygotes were clinically normal but their erythrocytes showed reduced osmotic fragility and occasional target cells.

ISSN:0363-0269    

DOI:10.3109/03630267809074781

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

摘要:

A table has been compiled of the spatial disposition of the amino acid residues in the human deoxy hemoglobin tetramer. The table also indicates regions of possible contact between residues in each subunit and possible contacts between subunits.

ISSN:0363-0269    

DOI:10.3109/03630267809074782

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267809074783

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267809074784

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267809074785

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267809074786

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267809074787

出版商:Taylor&Francis    

年代:1978

数据来源: Taylor