摘要:

The 8–93 SH groups were found to be present in freshly purified Hb Köln, but over a period of several days storage they gradually oxidized to form interchange disulfide bonds. It is suggested that this gradual oxidation accounts for the low SH values which have been reported. The SH groups of Hb Köln were also found to react more readily than normal with H2O2and O2

ISSN:0363-0269    

DOI:10.3109/03630267609031018

出版商:Taylor&Francis    

年代:1976

数据来源: Taylor

摘要:

Hemoglobin J Mexico has been found in five generations of a large Algerian family. Nine subjects have 55% Hb J although their parents, siblings and offspring may have 31%, the usual quantity found in heterozygotes. Those with 55t Hb J are considered to be homozygous for a chromosome carrying both a normal a chain locus and a locus forαJ. The proportion of the abnormal hemoglobin in all the subjects is in favor of an unequal expression of both loci, the amount of protein synthesis dissected by theαJgene being greater than that directed by theαA. In two heterozygotes a slightly higher proportion af the Hb J (38%) suggests the presence of a angle normal a chain locus in trans. An associatedα-thalassemia was excluded by biosynthetic studies in this family.

ISSN:0363-0269    

DOI:10.3109/03630267609031019

出版商:Taylor&Francis    

年代:1976

数据来源: Taylor

摘要:

This chromatographic procedure uses DEAE-cellulose as ion exchanger and glycine-KCN-NaCl solutions as developers. Blood samples from several adults and newborn infants withα,β,δ, orγchain variants have been analysed. The hemoglobins are eluted as compact and symmetrical zones, and the separation of many hemoglobin types is greatly improved. The procedure is relatively fast, simple, and inexpensive.

ISSN:0363-0269    

DOI:10.3109/03630267609031020

出版商:Taylor&Francis    

年代:1976

数据来源: Taylor

摘要:

A hemoglobin variant with the same electrophoretic mobility as employing S was found in three generations of a black family. No clinical symptoms or findings were present in subjects heterozygous for this mutant. Except for target forms of mature erythrocytes, they have no abnormal hematological findings. Structural studies demonstrated a previously under scribed substitution,β51Pro→Arg, in the abnormal fraction which accounts for about one-third of the total hemoglobin. This fraction is more unstable in vitro at 65°than normal A hemoglobin. Both whole blood and purified abnormal hemoglobin have increased oxygen affinity and a slightly decreased Bohr effect.

ISSN:0363-0269    

DOI:10.3109/03630267609031021

出版商:Taylor&Francis    

年代:1976

数据来源: Taylor

摘要:

On a field trip to Saudi Arabia (M. A. F. E. H.) in which the relationship betweenα-thalassemia and iron deficiency was studied, a fast moving hemoglobin variant was noted in a 30 year old Saudi Arabian woman. Analysis of the hemoglobin variant showed that the amino acid substitution wasβ120 Lys-Asn. This variant had not been described previously and has been named Hb Riyadh. There was also present anα-thalassemia and details are given of the imbalance of globin chain synthesis. It was possible to improve considerably the balance in vitro by the addition of hemin.

ISSN:0363-0269    

DOI:10.3109/03630267609031022

出版商:Taylor&Francis    

年代:1976

数据来源: Taylor

摘要:

In Thailand, two types of high 1% A2-B-thalassemia genes:βO-thalassemia (βO-thal) or classicalβ-thalnssemia andβ+-thalassemia (β+-thal) or mild 6-thalasseinin exist. This study presents hematologic data and globin chain synthesis in peripheral blood of the genuineβ+-thal hetcrozygotes in comparision with those of theβO-thal heterozygotes. Thirty individuals of Thai and Chinese exctraction with theβ+-thal heterozygosity were hematologically examined. The hematologic means of hemoglobin concentration, MLV, MCH, MCHC, 11h A2and alkali3denaturation hemoglobin of theβ+-thal traits were, 11.7 g%, 67.8 u3, 21.5γγ, 32.1%, 4.94% and 1.20% respectively. These were not statistically different from those of theβO-thal traits of our previous study(1). The globin chin synthesis in reticulocytes were performed by incorporation of3H-Leucine for 3 hours. The mean of total radioactivityα/βratio in 11 normal controls was 1.07±SD 0.03 The mean ofα/βratio in 9β+-thal traits was 2.03±SD 0.10 which was significantly different from that in 7βO-thal traits of 2.28±SD 0.07 Our globin chain synthesis thus appears to be helpful of discriminating theβ+-thal trait from theβO-thal trait.

ISSN:0363-0269    

DOI:10.3109/03630267609031023

出版商:Taylor&Francis    

年代:1976

数据来源: Taylor

摘要:

The amino acid substitution in a new homoglobin variant, Hb Baylor [α2β281(EF5) Leu-Arg], has been determined by application of column chromatography and amino acid analysis of the tryptic peptides. The hemoglobin is somewhat unstable and it has a high oxygen affinity. The porosities shows the opposing hematologic effects of these two properties-the instituionability being associated with increased destruction, and the high O2affinity with increased production, of erythrocytes.

ISSN:0363-0269    

DOI:10.3109/03630267609031024

出版商:Taylor&Francis    

年代:1976

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267609031025

出版商:Taylor&Francis    

年代:1976

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267609031026

出版商:Taylor&Francis    

年代:1976

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267609031027

出版商:Taylor&Francis    

年代:1976

数据来源: Taylor