摘要:

Procedures are described for the preparation of cyanogen bromide and maleyl peptides of theαandβchains of human Hemoglobin. These relatively large peptides have facilitated application of automated sequencing techniques to determine mutations fn the primary structure of abnormal hemoglobins.

ISSN:0363-0269    

DOI:10.3109/03630267709003406

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

摘要:

A screening survey for abnormal hemoglobins at a hospital in Mizunami city, Gifu prefecture, Japan detected a fast-moving variant of hemoglobin in a family of Japanese origin. The abnormal hemoglobin constitutes about 45 percent of the total hemoglobin from the propositus and another carrier in the family, but neither of these persons had anemia, jaundice, cyanosis or splenomegaly. Structural analysis of this hemoglobin revealed that the amino acid substitution vas at residue 83 in theβchain, where a glycine was replaced by an aspartic acid. Thin hemoglobin variant has been previously reported in a Greek child (hemoglobin Pyrgos) (1). Oxygen affinity of hemoglobin Pyrgos was found to be normal.

ISSN:0363-0269    

DOI:10.3109/03630267709003407

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

摘要:

An electrophoretically HbA-like hemoglobin component is produced In increasing amounts during storage in hemolysate preparations from macerated tissue (liver, kidney, spleen) of fetuses. Within twenty four hours after hemolysate preparation the“fast moving”fraction increases up to 40 per cent of total hemoglobin, while the concentration of HbA remains constant (5-7 %) in hemolysates obtained from peripheral blood of the same donor individuals. By structural studies (fingerprint and aminoacid analysis) the HbA-like component was identified as ai. artefact of HbF, characterized by the absence of the C-terminal arginine of theαchains. From experimental data it is concluded, that the break down product results from a digestion of HbF by carboxypeptidase B, the enzyme being released from the macerated tissues. Analogous to a modification of HbA, i.e. Hb Koelliker (α2minus 141 Argβ2), the structure of the degradaeion product of Hbf isα2minus 141 Argγ2(HbF Koelliker).These findings should be considered when hemolysate preparations contaminated with tissue cells are used for investigations on developmental hemoglobins especially by electrophoretic or chromatographic methods.

ISSN:0363-0269    

DOI:10.3109/03630267709003408

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

摘要:

Hb Gun Hill has been found in an asymptomatic black female living in Alabama; neither her parents nor her two siblings carry the variant. The deletion of five mino acid residues in theβchain of lib Gun Hill (α2β291-95 deleted) causes this vartant to have a high oxygen affinity, an interaction coefficient of unity and an absence of the Bohr effect. The rate of oxidation of liganded Hb Gun Hill by the ferricyanide ion follovs first-order kinetics. Hb Gun Hill is to a great extent dissociated into dimers over a wide range of pH values.

ISSN:0363-0269    

DOI:10.3109/03630267709003409

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267709003410

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267709003411

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

摘要:

Ihmoglobin Setif has been reported previously in an Algerian family and has now been located in Iran, where it was found in 9 unrelated families within the last two years.

ISSN:0363-0269    

DOI:10.3109/03630267709003412

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267709003413

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

摘要:

Human Haemoglobin Variants and Their Characteristics H. Lehmann and P.A.H. Kynoch, Elsevier/North-Holland, Inc., New York, NY, 1976 vi+242 pages US $23.25/Df1.60.00 ISBN 0-7204-0585-8

ISSN:0363-0269    

DOI:10.3109/03630267709003414

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor

ISSN:0363-0269    

DOI:10.3109/03630267709003415

出版商:Taylor&Francis    

年代:1977

数据来源: Taylor