ISSN:1424-8581    

DOI:10.1159/000132898

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

The single-copy parathyroid hormone-like peptide gene (Pthlh) was assigned to mouse chromosome 6 using a rat PTHLH cDNA as hybridization probe in Southern blot analysis of DNAs isolated from a panel of mouse × Chinese hamster cell hybrids. The mouse parathyroid hormone gene (Pth) has previously been assigned to mouse chromosome 7 and the PTHLH nd PTH genes have also been shown to be on different chromosomes in human and rat. Therefore, despite significant amino-terminal sequence homology between the PTHLH and PTH peptides, as well as similarities in the structural organization of the human PTHLH and PTH genes, the genes encoding these peptides have discrete chromosomal locations in the mouse, rat, and man

ISSN:1424-8581    

DOI:10.1159/000132899

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Using a rat × mouse somatic cell hybrid that contains chromosome 11 as the only mouse material, we have shown that myeloperoxidase, which maps to human chromosome 17, maps to mouse chromosome 11. Regional assignment of the gene by in situ hybridization localized Mpo to the region C-El, with a peak at band 11C. These results further confirm and extend observations on the remarkable homology between human chromosome 17 and mouse 11

ISSN:1424-8581    

DOI:10.1159/000132900

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

The majority of Ewing sarcomas and peripheral neuroectodermal tumors (PNET) that have been karyotyped contain a specific translocation, t(11;22)(q23;q11). We report here a second nonrandom translocation, der( 16)t(1; 16)(q21;q13), in 2 of 20 cases of Ewing sarcoma (seven previously unreported) and 2 of 7 cases of PNET (all previously unreported). All cases with this translocation also contained the t(l 1;22). Comparison of C-banding patterns in tumor and peripheral lymphocyte karyotypes in one case indicated that the likely breakpoints were lq21 and 16q13. The presence of this translocation in cell lines will enable further investigation of the molecular events important in the pathogenesis of Ewing sarcoma and PNET.

ISSN:1424-8581    

DOI:10.1159/000132901

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Phosphorylase kinase is a glycogenolytic enzyme in several animal tissues. Within the last few years all four subunits of the enzyme have been cloned. The β, γ, and δ subunits are known to be autosomal. We have mapped the α subunit of phosphorylase kinase, recently cloned by Zander et al. (1988), in an interspecific mouse pedigree and localized it on the X chromosome, where it maps between the X-linked zinc finger protein and phosphogly cerate kinase genes, close to the latter. In man and mouse several X-linked disorders of this enzyme have been described. Although the X-linked phosphorylase kinase deficiency in mice may be caused by a mutation in the structural gene for the α subunit, mapped here, the existence of a separate regulatory locus, important in the normal expression or function of the enzyme in muscle, still remains a possibi

ISSN:1424-8581    

DOI:10.1159/000132902

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Previously, we assigned the αA2-crystallin (Crya-1) structural gene to mouse chromosome 17 via Southern blot hybridization analysis of mouse × Chinese hamster somatic cell hybrids. Using in situ hybridization, we have now localized this gene to 17A3→B, a subchromosomal region containing several genes whose linkage relationships have been shown to be conserved on human chromosome 6. In man, however, the homologous gene (CRYA1) is located on human chromosome 21, indicating that internal rearrangements can occur within highly conserved chromosomal regions during the divergence of man and mo

ISSN:1424-8581    

DOI:10.1159/000132903

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Spermatocytes from the mole, Talpa occidentalis, a species that includes both XX males and intersexes, were surface-spread and silver-stained to substage meiotic prophase from early zygonema through pachynema. In zygonema, only the Z2 and Z3 substages were found. This stage differed in comparison with such species as the Chinese hamster, laboratory mouse, and deer mouse, which belong to orders other than Insectivora. Pachynema, in which five substages were established (P1-P5), seems to be a more homogeneous stage, and remarkable differences with respect to the above-mentioned species were not found. Synaptic adjustment was demonstrated in X-Y pairing. Nonhomologous pairing was evident at the Y-centromeric region and considered likely in the proximal arm of this chromosome. In addition to sequencing the events taking place during zygonema and pachynema in males from a wild population in which some members show sex reversal, our finding represents the first attempt to substage zygonema and pachynema in an Insectivore species, thus contributing to current knowledge of the nature and degree of variability in the mammalian synaptic process.

ISSN:1424-8581    

DOI:10.1159/000132904

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

The human sperm/hamster egg fusion technique has been used to analyse 6,821 human sperm chromosome complements from 98 men to determine if all chromosomes are equally likely to be involved in aneuploid events or if some chromosomes are particularly susceptible to nondisjunction. The frequency of hypohaploidy and hyperhaploidy was compared among different chromosome groups and individual chromosomes. In general, hypohaploid sperm complements were more frequent than hyperhaploid complements. The distribution of chromosome loss in the hypohaploid complements indicated that significantly fewer of the large chromosomes and significantly more of the small chromosomes were lost, suggesting that technical loss predominantly affects small chromosomes. Among the autosomes, the observed frequency of hyperhaploid sperm equalled the expected frequency (assuming an equal frequency of nondisjunction for all chromosomes) for all chromosome groups. Among individual autosomes, only chromosome 9 showed an increased frequency of hyperhaploidy. The sex chromosomes also showed a significant increase in the frequency of hyperhaploidy. These results are consistent with studies of spontaneous abortions and liveborns demonstrating that aneuploidy for the sex chromosomes is caused by paternal meiotic error more commonly than aneuploidy for the autosomes.

ISSN:1424-8581    

DOI:10.1159/000132905

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

We conducted Southern blot analysis of DNAs from mouse × hamster somatic cell hybrids and progeny of an interspecies backcross to localize the mouse gene for DNA polymerase β (Polb) to the centromeric end of Chromosome 8 These results provide additional support for the regional localization of polymerase β. on the short arm of human chromosome 8 or the veryproximal end of the long a

ISSN:1424-8581    

DOI:10.1159/000132906

出版商:S. Karger AG    

年代:1990

数据来源: Karger

摘要:

Murine methylmalonyl CoA mutase (Mut) has been localized to chromosome 17C-D by in situ hybridization in cell lines containing a 2.17 Robertsonian translocation. This locus, which was mapped with the help of a murine methylmalonyl CoA mutase cDNA probe, and others on murine chromosome 17 are syntenic, though not necessarily colinear, with loci on human chromosome 6.

ISSN:1424-8581    

DOI:10.1159/000132907

出版商:S. Karger AG    

年代:1990

数据来源: Karger