摘要:

Two proto-oncogenes, Myc and Sis, as well as a putative mouse oncogene, int-1, were localized by in situ hybridization to the distal third of mouse chromosome 15. The respective loci of these genes map to different cytogenetic sites with Myc in 15D2-D3, Sis in 15E, and int-1 in 15F1-F3. These data may be of considerable impact as to the correlation of mouse neoplasias with chromosomal aberrations involving chromosome 15.

ISSN:1424-8581    

DOI:10.1159/000132345

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Chromosome analysis was done in salamanders belonging to the genus Hynobius using the R-banding (RBG) technique. The embryonic cells of H. leechii Boulenger from Korea and H. nebulosus (Schlegel), H. tokyoensis Tago, H. nigrescens Stejneger, and H. retardatus Dunn from Japan were cultured in medium containing 5-bromodeoxyuridine. R-banding patterns in these five species were analyzed, except for most of the small-sized chromosomes. Eighteen out of 28 chromosome pairs in H. leechii, H. nebulosus, H. tokyoensis, and H. nigrescens, and 16 out of 20 chromosome pairs in H. retardatus could be identified by their R-banding patterns. The R-banding patterns in H. leechii, H. nebulosus, H. tokyoensis, and H. nigrescens were very similar to one another as are their karyotypes. Among these four species, about 90% of the total lengths of the karyotypes in each species showed homoeologous R-banding patterns, while among all the five species, including H. retardatus, homoeologies decreased to about 65%. The results imply that H. leechii, H. nebulosus, H. tokyoensis, and H. nigrescens are extremely close to one another phylogenetically, and that H. retardatus is a near phylogenetic relation to the other species of this study.

ISSN:1424-8581    

DOI:10.1159/000132346

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Molecular probes were used as markers in the backcross (Czech II × BALB/cPt) × Czech II to determine the positions of six genes on mouse chromosome 16 (MMU 16). The order of the genes mapped is (centromere), protamine-1 (Prm-1), immunoglobulin λ1 light chain (Igl-1), preprosomatostatin (Smst), an endogenous mouse mammary tumor virus locus (Mtv-6), and two more distal sequences, superoxide dis-mutase, cytoplasmic form (Sod-1), and the proto-oncogene sequence Ets-2. The largest recombination frequency between any two adjacent markers is 24 cM, and thus the position of any marker on MMU 16 that is polymorphic between these two strains can be readily determined in this backcross. A region of MMU 16 which corresponds to the Down syndrome region of human chromosome 21 is located near the distal end of the chromoso

ISSN:1424-8581    

DOI:10.1159/000132347

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Eighteen genes were assigned to chromosomes in the sacred baboon, Papio hamadryas, by their concordant segregation with the chromosomes in a set of baboon × Chinese hamster somatic cell hybrids. ACY1 was assigned to P. hamadryas chromosome 2 (PHA 2); SOD1 and MDH2 to PHA 3; ME1 and SOD2 to PHA 4; NP, MPI, PKM2, and HEXA to PHA 7; PP to PHA 9; ADA and ITPA to PHA 10; LDHB and TPI1 to PHA 11; MDH1 to PHA 13; ESD to PHA 17; and GPI and PEPD to PHA 20. Regional assignments were possible for ACY1 (PHA 2pter→q1) and MDH2 and SOD1 (PHA 3p). Five other independently segregating markers or syntenic groups (PGD, PGM 1; and PEPC; PGM2 and PEPS; IDH1; LDHA and ACP2; and GSR) were also identified. Gene assignments and syntenic groups described in P. hamadryas are compared to those found in P. papio, the rhesus monkey, and man. A possible primate model for human lymphoid disease is discuss

ISSN:1424-8581    

DOI:10.1159/000132348

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Starch gel electrophoresis of Harris’ hawk muscle samples revealed an unusual association of enzyme phenotypes and sex: all females were heterozygous and all males were homozygous. Enzyme activities were not significantly different between sexes. Since birds are characterized by female heterogamety (ZZ males, ZW females), these results are interpreted as suggesting sex linkage with the expression of both Z- and W-linked allele

ISSN:1424-8581    

DOI:10.1159/000132349

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Mouse-Chinese hamster hybrids segregating mouse chromosomes were analyzed by Southern hybridization techniques to map the genes for somatostatin (Smst), glucagon (Gcg), calcitonin (Calc) and parathyroid hormone (Pth). The mouse gene for somatostatin, detected on a 20-kb EcoRl fragment, is located on mouse chromosome 16. Glucagon cDNA hybridized to a 14-kb EcoRl fragment residing on chromosome 2. Calcitonin and parathyroid hormone genes, detected on 7.8-kb HindIII and 6.0-kb BamHI fragments, respectively, were on mouse chromosome 7. The calcitonin and parathyroid hormone genes appear to be part of a larger linkage group which has been conserved in mouse and man.

ISSN:1424-8581    

DOI:10.1159/000132350

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Using a panel of mouse × rat somatic cell hybrids and a characterized rat vitamin D binding protein (DBP) cDNA probe, we assigned the gene coding for rat DBP to chromosome 14. We conclude that the genes for DBP, albumin, and α-fetoprotein, all of which derive from a common precursor, are syntenic in the rat, as they are in ma

ISSN:1424-8581    

DOI:10.1159/000132351

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

A cell line hemizygous for a deletion of the human chromosome region 7q22→q32 was used for fine mapping three probes closely linked to the cystic fibrosis locus. The three markers, J.3.11, 7c22, and met, were all found to be deleted from the region 7q22→q32. This finding, in combination with previously published mapping data, led to the assignment of J3.11 to 7

ISSN:1424-8581    

DOI:10.1159/000132352

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

Synaptonemal complex analyses were carried out by electron microscopy on surface-spread spermatocytes of one normal bull and two bulls that were heterozygous for the so-called 1;29 translocation. The autosomal bivalents of the normal karyotype, which could be arranged by size in a series, demonstrated kinetochores at the terminally located attachment plaques. One autosomal bivalent was clearly larger than the rest and apparently consisted of the long arm of the 1;29 translocation. The 1;29 translocation was the longest autosome in the set and had a kinetochore in a subtelocentric position. Some of the autosome pairs had nucleolus organizer regions in telomeric regions. The X and Y chromosomes, which were not paired at zygotene, demonstrated association in a very short segment at early pachytene; in no cells could a synaptonemal complex be seen between the X and Y. Very often the sex chromosomes were dissociated. At zygotene, a few, usually large, bivalents were unpaired proximally. This always also involved the proximal parts of the arms of the 1;29 translocation and their normal homologs. At early pachytene, the 1;29 trivalent, although to a less extensive degree, was also unpaired in the pericentric region. Configurations in which one chromosome, either 1 or 29, was completely paired with its corresponding arm in the 1;29 translocation chromosome also occurred. When unpaired proximally, the size of chromosome 1 agreed fairly well with the size of its corresponding arm, but the size of chromosome 29 was considerably larger than the corresponding arm of the 1;29 translocation chromosome. During late zygotene and early pachytene, the percent difference between chromosome 29 and its corresponding arm decreased, and at mid and late pachytene there had been a complete synaptic adjustment. The size difference and pairing behavior indicated that a deletion of the kinetochore and the most proximal segment of chromosome 29 had preceded the fusion with chromosome 1 into the 1;29 translocation. The unique structural appearance of the 1;29 translocation chromosome compared to that of other centric fusion translocations in cattle lends support to the theory of a monophyletic origin of the 1;29 translocation. The importance of the pairing behavior observed in governing recombination and chromosome disjunction is briefly discussed.

ISSN:1424-8581    

DOI:10.1159/000132353

出版商:S. Karger AG    

年代:1987

数据来源: Karger

摘要:

The D20S6 locus has been sublocalized by in situ hybridization using the pD3H12 probe to human chromosome band 20p12 and the D20S4 locus using the pMSl-27 probe to 20q13.2. A rare new restriction fragment length polymorphism detected in MspI-digested DNA by the pMSl-27 probe is reported. Linkage studies in nine families have shown that the D20S6 locus is linked to D20S5 (formerly mapped to 20p12 by in situ hybridization) with a maximum likelihood estimate of 0.07 for the recombination frequency (lod score = 9.07) and a confidence interval of 0.02 to 0.14. Estimated recombination frequencies were similar in males and females. Using both two- and multipoint analyses, linkage of D20S4 with the D20S5 and D20S6 loci was excluded and the suggested order for the three loci on chromosome 20 is D20S5-D20S6-centromere-D20S4. D20S5 and D20S6 are very useful markers for linkage studies because of their close proximity and reasonably good polymorphic information content values.

ISSN:1424-8581    

DOI:10.1159/000132354

出版商:S. Karger AG    

年代:1987

数据来源: Karger