ISSN:1424-8581    

DOI:10.1159/000133601

出版商:S. Karger AG    

年代:1994

数据来源: Karger

ISSN:1424-8581    

DOI:10.1159/000133602

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

We have isolated the human α-catenin gene (CTNNAl), which encodes a cadherin-associated protein, and have determined its primary structure and chromosomal localization. The transcript of CTNNAl is 3.4 kb long and consists of 16 coding exons encoding 906 amino acids and at least one 5’ noncoding exon. The 102-kDa predicted protein is the same size as the murine homolog, and the amino acid sequences of the two proteins are 99.2% homologous. Analysis by reverse transcription-PCR revealed that this gene is expressed ubiquitously in normal tissues. It was mapped to chromosome band 5q31 by fluorescent in situ hybridizati

ISSN:1424-8581    

DOI:10.1159/000133603

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

Fixed metaphase chromosomes of the eel Anguilla anguilla were treated with the restriction enzymes Haelll, HinfI, and MseI. All three restriction endonucleases are capable of inducing a G-band-like pattern if the incubation time and enzyme concentration are controlled. The results are discussed in relation to the main factors involved in the appearance of serial G-bands. Chromatin organization, rather than DNA composition, seems to account for the interstitial G-band pattern exhibited by these eel chromosomes.

ISSN:1424-8581    

DOI:10.1159/000133604

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

Fluorescence in situ hybridization (FISH) techniques were applied to the mapping of 18 probes from chromosome 18, permitting seven new assignments: D18S16 (18p11.31), D18S12 (18p11.1), D18S1 (18q12.2), D18S13 (18q21.31), D18S18 (18q21.31), D18S14 (18q21.33), and D18S17 (18q23). In addition, the localization of D18S3 in 18p11.3 was confirmed and that of D18S6, previously mapped in 18p11, was changed to 18q21.13. Finally, a more accurate mapping for nine probes was proposed: D18S7 (18q12.2), D18S10 (18q12.2), D18S24 (18q21.13), D18S8 (18q21.13), GRP(18q21.31), BCL2(18q21.33), D18S5(18q22.1), D18S19 (18q22.1), and D18S11 (18q22.3). The ordering of probes located in close proximity was made possible by combined use of single-color FISH with direct assignment on banded chromosomes, chromosomal length measurements, and double-color FISH.

ISSN:1424-8581    

DOI:10.1159/000133605

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

Sixty-three human brain cDNA sequences were newly assigned to individual human chromosomes. Ten of these were subregionally localized, and one was also mapped in the mouse genome. Four previously reported assignments were refined. PCR primers were designed from expressed sequence tags (ESTs) and tested for specific amplification from human genomic DNA. DNA was then amplified, often in multiplexed PCR reactions, using DNA from somatic cell hybrid mapping panels as templates. The amplification products were identified using an automated fluorescence detection system. Chromosomal assignments were made by discordancy analysis. Thirteen newly localized cDNAs exhibited homology to previously reported sequences. EST01471 was shown to correspond to human microtubule-associated protein 1B (MAP1B), confirming the previous mapping of this gene to human chromosome 5. Other genes tentatively assigned to chromosomes based on these results were a component of the signal peptide receptor of the endoplasmic reticulum (EST00745) and a cyclic AMP-regulated phosphoprotein (EST01041) on chromosome 1, a protein phosphatase 2A 55-kDa regulatory subunit (EST01650) on chromosome 4, an NAD(P) transhydrogenase (EST01744) on chromosome 5, ribosomal proteins LI a or Lib (EST01627) and L18a (EST01583), a brain transcription factor (BF-1, EST00795) on chromosome 14, a milk fat globule membrane-related protein (EST01678) on chromosome 15, a putative peptide initiation factor (EST00675) on chromosome 17, thiosulfate sulfurtransferase (TST) on chromosome 22, and moesin (MSN) (EST00896) and a human equivalent of rat spot 14 (S14) (EST00887) on Xp11→cen and Xpter→p21.3, respectiv

ISSN:1424-8581    

DOI:10.1159/000133606

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

Localization of alphoid human satellite DNAs using fluorescence in situ hybridization (FISH) of metaphase chromosomes following treatment with 5-azacytidine to produce undercondensation showed that human α-satellite DNA is not sensitive to the condensation-inhibition effect of 5-azacytidine. The difference in heterochromatic DNA subsets was particularly evident in the constitutive heterochromatin of chromosomes 1 and 9. Comparison of the results obtained after FISH with those obtained from electron microscopy and G-banding enabled accurate localization of this DNA domain

ISSN:1424-8581    

DOI:10.1159/000133607

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

Fluorescence in situ hybridization (FISH) with chromosome-specific repetitive DNA probes provides a new method for rapid detection of aneuploidy in human sperm. There is widespread interest in this technique for basic research, as well as for screening men exposed to potential aneugens. A number of laboratories have reported a wide range in the frequency of aneuploidy for chromosomes in human sperm, suggesting that FISH may not reflect the true frequency accurately. We have serendipitously discovered that the length of time that fixed frozen sperm nuclei are stored affects both the hybridization efficiency and disomy frequency of individual chromosomes. This may explain some of the variation in the aneuploidy frequencies observed among laboratories.

ISSN:1424-8581    

DOI:10.1159/000133608

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

We have developed a procedure for efficient in situ hybridization of bacterial recombinants created with various types of large-insert cloning vectors. Minimal quantities of crude DNA are amplified and labeled during the degenerate-oligonucleotide-primed polymerase chain reaction. The resulting probes generate high-intensity fluorescent hybridization signals on metaphase chromosomes and on interphase nuclei.

ISSN:1424-8581    

DOI:10.1159/000133609

出版商:S. Karger AG    

年代:1994

数据来源: Karger

摘要:

Tear prealbumin, a major protein of the human tear fluid, is also present in several other human body secretions. Recently it was shown to be a member of the lipocalin superfamily, a class of proteins that function as carriers for small hydrophobic molecules. Using a genomic tear prealbumin probe, we have mapped the gene (LCN1) to human chromosome 8q24 by fluorescent in situ hybridization.

ISSN:1424-8581    

DOI:10.1159/000133610

出版商:S. Karger AG    

年代:1994

数据来源: Karger