摘要:

Genome size was measured as the amount of Feul-gen-stained DNA in six species of the family Hylobatidae and in a hybrid of the gibbon (Hylobates muelleri) and siamang (Symphalangus syndactylus). The family, on the whole, exhibits a wider range of genome sizes than pongids; in particular, the siamang has about 15% more DNA than the 44-chromosome Hylobates species of the “lar” group. Quantitative analysis of C-heterochromatin in hybrid metaphases showed that the difference in genome size of the parental species correlates with the amount of C-band-positive material. Hylobatids are the only group of primates in which karyotype diversification has taken place with a massive quantitative change in constitutive heterochroma

ISSN:1424-8581    

DOI:10.1159/000132492

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

This paper describes a molecular investigation of a woman with an apparent large pericentric inversion of chromosome 5, inv(5)(pl4;q35), and one normal chromosome 5 and her child, who was born with cri-du-chat syndrome. The four chromosome 5 homologs from the proband and his mother were isolated in somatic cell hybrids, and their haplotypes were determined at nine loci polymorphic for restriction enzyme sites. The deleted chromosome in the proband was shown to carry alleles from both maternal homologs, verifying molecularly that a meiotic recombination event in the mother gave rise to her son’s deleted chromosome 5. The single crossover was presumably near the centromer

ISSN:1424-8581    

DOI:10.1159/000132493

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

Hybrid human-mouse T-cell clones reactive with Tp40 antibody, which detects cluster of differentiation (CD)7 antigen on human T lymphocytes, were established. Karyotype analysis showed that human chromosome 17 was essential for the expression of CD7 antigen. The presence of this chromosome was confirmed by enzyme analysis of galactokinase, which is coded by a gene on human chromosome 17.

ISSN:1424-8581    

DOI:10.1159/000132494

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

Two bcl-2 alleles have been identified in inbred strains of mice by restriction fragment length polymorphism (RFLP). Analysis of a bcl-2 RFLP in a series of bilineal congenic strains (C.D2), developed as a tool for chromosomal mapping studies, revealed linkage of bcl-2 to the Idh-1/Pep-3 region of murine chromosome 1. The co-segregation of bcl-2 alleles with allelic forms of two other chromosome 1 loci, Ren-1,2 and Spna-1 in a set of back-cross progeny, positions bcl-2 7.8 cM centromeric from Ren-1,2.

ISSN:1424-8581    

DOI:10.1159/000132495

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

Lactotransferrin (LTF) is an important member of the transferrin family of proteins. These proteins play an essential role in the transport of iron in extracellular fluid (Aisen and Lis-towsky, 1980). Southern blot analysis of mouse-human somatic cell hybrids have localized the LTF gene to region q21→qter of human chromosome 3 (Teng et al., unpublished data). Using the same full-length mouse cDNA probe (2.2 kb), the LTF gene was mapped to human chromosomal bands 3q21→q23 by in situ hybridization. The sublocalization of the LTF gene to 3q21→q23 is in the region of human chromosome 3 where the gene loci of transferrin and transferrin receptor have been localized (Yang et al., 1984; van de Rijn et al.,

ISSN:1424-8581    

DOI:10.1159/000132496

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

There is evidence for more than one α1 acid glycoprotein (α1AGP) gene, and they all appear to be in close proximity. In situ hybridization of the cloned human cDNA pα1AGP-2 to human chromosomes indicates that the α1AGP genes are located between bands q31 and q34.1 on chromosome 9. This finding is in agreement with the previous assignment of the locus for αAGP to a linkage group with ABO and AK on chromoso

ISSN:1424-8581    

DOI:10.1159/000132497

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

Differential activity of rRNA gene clusters following growth-hormone administration has been demonstrated in cultured lymphocytes from subjects with different genetic backgrounds, i.e., with or without in vivo peripheral responsiveness to the hormone. The influence of different culture conditions on ribosomal gene responsiveness was also tested. Ribosomal geneactivity was evaluated by selective silver staining of nucleolus organizing regions. The results show that hormone-induced enhancement of transcriptional activity requires both genetically determined cell responsiveness and environmentally determined permissive factors.

ISSN:1424-8581    

DOI:10.1159/000132498

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

An inverted and a satellited Y chromosome detected in peripheral blood lymphocytes of a chimpanzee and a pygmy chimpanzee, respectively, were characterized cytogenetically by various banding techniques. A detailed comparison with an inverted and a satellited Y chromosome in man suggested that the corresponding aberrations were homoeologic.

ISSN:1424-8581    

DOI:10.1159/000132499

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

We have analyzed structural aberrations in 5,000 sperm chromosome complements obtained from 20 men over a 5-yr period by fusion of human sperm with hamster eggs. Detailed data are presented on 366 abnormal cells with 379 analyzable breakpoints. The frequency of cells with structural aberrations ranged from 1.9% to 14.5% among donors; this interindividual variability was statistically significant (p < 0.0001). In contrast, repeat samples from individual men showed no significant variation over time. The number of sperm chromosome sets processed per hamster egg had no effect on the frequency with which structural aberrations occurred, nor were sperm chromosome abnormalities altered by varying capacitation or culture conditions. The spectrum of structural aberrations observed in human sperm chromosomes and a chi-square analysis of breakpoints based on DNA content are presented. Although human sperm chromosome abnormalities were visualized with a cross-species system, we believe that they represent an inherent, biologically significant phenomenon.

ISSN:1424-8581    

DOI:10.1159/000132500

出版商:S. Karger AG    

年代:1988

数据来源: Karger

摘要:

The chromosomal location of the human gene coding for both the β-subunit of prolyl 4-hydroxylase (P4HB) and the enzyme disulfide isomerase (PDI) was determined using mouse × human somatic cell hybrids and three different methods for identifying either the human P4HB/PDI protein or the respective gene: immunoblotting with species-specific monoclonal antibodies; radioimmunoassay with species-specific polyclonal antibodies; and (3) Southern blotting after cleavage of the DNA with EcoRI, HindIII, or BamHI, followed by hybridization with a mixture of two cDNA probes for human P4HB. All three methods gave identical data, demonstrating complete cosegregation of the human protein or its gene in all 17 cell hybrids tested with human chromosome 17. A cell hybrid lacking an intact chromosome 17 localized the gene to 17p11→q

ISSN:1424-8581    

DOI:10.1159/000132501

出版商:S. Karger AG    

年代:1988

数据来源: Karger