摘要:

SUMMARYWith indirect immunofluorescence techniques we demonstrated that recombinant gamma‐interferon induced the expression of the class II antigens HLA‐DR and HLA‐DQ as well as intercellular adhesion molecule‐1 (ICAM‐1) on normal, cultured human keratinocytes grown in low‐calcium, serum‐free medium. Each antigen displayed a distinctive cellular staining pattern. HLA‐DR was strongly localized to perinuclear zones with intense cell surface expression; HLA‐DQ displayed a perinuclear accentuation, but with minimal cell surface staining, and ICAM‐1 was strongly expressed in a diffuse cytoplasmic pattern with intense cell surface expression. Keratinocytes grown in medium supplemented with 10% fetal calf serum underwent differentiation, with a diminished expression of all three antigens as compared to those grown in low‐calcium, serum‐free medium.These results confirm that gamma interferon can differentially regulate HLA‐DR nd HLA‐DQ expression; that there are probably different biochemical metabolic pathways by which these three molecules are expressed on keratinocytes, and that the expression is also a function of the degree of keratinocyte differentiation. The strong cell surface expression of ICAM‐1 is suggested to be of major importance as the recognition molecule, by which T cells bind to gamma interferon exposed keratinocytes, and suggests and integral role for this molecule in epi

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb07759.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYQuantitative changes in the levels of keratin polypeptides extracted from keratotome shavings from psoriatic epidermis were measured by using one‐dimensional SDS‐PAGE, followed by scanning densitometry. Values obtained were compared with results for non‐lesional epidermis and from epidermis from normal individuals. Patients on four different treatment regimens were investigated by repeated sampling over 3–4 months starting before therapy commenced. The levels of four keratins changed significantly: keratins 1 (70kd) and 2 (66kd) tended to rise to normal levels, while keratins 16 (50 kd) and 18 (44 kd) fell to normal levels. There were differential effects as well as differences in the rates of normalization depending upon the treatment regimen. The most rapid normalization of the levels of all four keratins was observed with topical dithranol (anthralin) treatment (five patients) with plaque resolution and keratin level normalization after 7–9 weeks. Oral hydroxyurea (three patients) had similar effects, but over a longer time scale (20 weeks). In contrast, oral etretinate (four patients) caused a normalization of all except keratin 2 (66 kd) over a period of 20–28 weeks, and keratin 1 (70 kd) levels tended to ‘overshoot' the normal level. PUVA (five patients) caused rapid normalization (in 9–12 weeks) of keratins 2 (66 kd) and 18 (44 kd), but had much weaker effects on keratins 1 (70 kd) and 10 (57 kd). These results suggest that resolution of lesions as judged by clinical criteria can occur without normalization of the keratin electrophoretic profile. Possibly the most reliable marker of clinical resolution was the reduction in keratin 16 (50 kd), since treatment effects on the differentiation of keratins 1 (70 kd) and 2 (66 kd)

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb07760.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYVitamin D3and its active metabolite 1α‐25‐dihydroxyvitamin D3(1α‐25‐(OH)2D3) have been reported to play a role in melanogenesis. Physiological concentrations of 1α‐25‐(OH)2D3) were found to acutely elevate intracellular free calcium (using Fura 2) in B16 primary (1°) cells. Membrane phosphoinositide turnover was unaffected by 1α‐25‐(OH)2D3. The rise in intracellular free calcium was entirely dependent on extracellular calcium and was not mimicked by vitamin D3. However, in neither B16‐1° nor B16‐F1melanoma cells did vitamin D3or 1α‐25‐(OH)

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb07761.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYPossible early side‐effects of retinoid treatment on bones were studied with the aid of bone‐scintigraphy. Isotretinoin treatment (1.0 mg/kg daily for 4 months) was given to 18 patients with acne. Fifteen patients with psoriasis received etretinate treatment (0.7–1.0 mg/kg/day) for 4 months. In the group treated with isotretinoin, pathological uptake of radiolabel was found in three cases, while in the group treated with etretinate, no bone changes attributable to treatment were found. During isotretinoin treatment, a decrease in growth‐plate activity was observed. Bone‐scintigraphy is considered to be a suitable method for early screening for bone changes occurring in retinoid

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb07762.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe endonuclease digest patterns of viral DNA from 48 genital and 45 non‐genital molluscum contagiosum virus (MCV) lesions were examined. The overall ratio of MCVI to MCVII was 3.23:1. There was no predominance of either MCV type in genital lesions. No obvious morphological differences were seen between MCVI and MCVII lesions. MCVII was not found in any patient under 15 years ol

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb07763.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThe lipid content of human sweat was determined in thermally induced sweat collected over a Vaseline® or silicone barrier placed on the skin (clean sweat) and in sweat scraped from the skin surface without a barrier coating (scraped sweat). Lipids were extracted from concentrated sweat samples into chloroform:methanol and estimated by thin‐layer chromatography in conjunction with photodensitometry. Scraped sweat contained 4 to 10 times more lipid than clean sweat and included cholesterol sulphate and ceramides resembling those found in the stratum corneum. In contrast, clean sweat contained only small amounts of free fatty acids and sterol. A marked individual and daily variation in sweat lipid content was also noted. The study indicates the importance of avoiding epidermal contamination when collecting sweat and the usefulness of our sweat collection meth

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb07764.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYHomogenates of normal human epidermis synthesized 12‐hydroxyeicosatetraenoic acid (12‐HETE) when incubatedin vitrowith arachidonic acid. The stereoconfigurations of the C‐12 hydroxyl isomers were determined by incubation with potato 5‐lipoxygenase. The synthesized substrate‐specific diHETEs; 5S, 12R and 5S, 12S, were readily separated by high performance liquid chromatograpy. Using this novel methodology, the normal epidermis was found to synthesize predominantly 12‐S‐HETE while, in contrast, psoriatic scale was found to contain 12‐R‐HETE. The 5‐lipoxygenase inhibitors, Merck L‐651, 896, Takeda AA861, and the active metabolite of Syntex lonapalene were found to inhibit 12‐HETE formation in norma

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb07765.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYSpecific antibodies present in the sera of patients with bullous pemphigoid or pemphigus vulgaris were detected in an enzyme‐linked immunosorbent assay (ELISA) employing a squamous carcinoma cell line, SCaBER, as substrate. Bullous pemphigoid sera bound preferentially to permeabilized cells, suggesting that the antigens are largely intracellular. The assay may prove to be a useful addition to current methods of detecting circulating antibodies in these patient

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb07766.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYPassive intestinal permeability was measured in 15 adult patients with atopic eczema. Values in the patients did not differ significantly from those in 12 healthy control subjects. These results suggest that altered intestinal permeability is not important in the pathogensis of atopic eczema in adults.

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb07767.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY

摘要:

SUMMARYThirty‐four per cent of 2110 patients with eczema attending a contact dermatitis clinic had hand eczema. Fifty‐five per cent (395 of 721) had contact dermatitis and 45% (326 of 721) had endogenous/unclassifiable eczema. Thirty‐five per cent of cases (217 of 721) were occupational eczema. An epidemiological comparison of patients with occupational and non‐occupational hand eczema (defined as eczema occurring on the hands up to the wrist line) was made. Hand eczema was more common in males in both groups, and there was a significantly larger proportion of males in the occupational group (65%) than the non‐occupational group (51%) (P<0.001). The prevalence of a personal or family history of atopy was significantly lower in the occupational group (7%) than the non‐occupational group (15%) (P<0.005). Irritant contact dermatitis occurred in a larger proportion of patients in the occupational group (76%) than the non‐occupational group (39%) (P<0.0001). There was no significant difference between the proportions of patients with positive patch test reactions in the occupational group (34%) and the non‐occupational group (41%). Potassium dichromate, and epoxy resin allergy occurred significantly, more frequently, in the occupational group whereas fragrance‐mix allergy was significantly more frequent in the non

ISSN:0007-0963    

DOI:10.1111/j.1365-2133.1989.tb07768.x

出版商:Blackwell Publishing Ltd    

年代:1989

数据来源: WILEY