ISSN:0001-6772    

DOI:10.1111/j.1748-1716.1940.tb00249.x

出版商:Blackwell Publishing Ltd    

年代:1940

数据来源: WILEY

摘要:

Summary.A method of blood volume determination based on the determination of the dilution of labelled corpuscles is described. Radioactive sodium phosphate is administered to rabbit B; after the lapse of some days, a known blood sample of this rabbit is injected into the vein of rabbit A. A few minutes later, the corpuscles of a known volume of the blood of rabbit A are secured, their phosphatide content extracted, and its activity measured. Corpuscles of a known blood volume of rabbit B are treated in the same way. From the ratio of the labelled phosphatide P content of the corpuscles of rabbit A and rabbit B, the total blood volume of rabbit A is calculated.An alternative and often preferable determination is based on the comparison of the activity of the acid‐soluble P secured from the corpuscle samples of rabbit A and rabbit B.The blood volume per kg of rabbit weight is found to be 42 c.c., per kg of chick weight 45 c.c.We wish to express our hearty thanks to Professor NielsBohrfor putting numerous facilities at our disposal, to Professor ErnestO. Lawrencefor the gift of the radioactive phosphate, and to Dr. H. Damfor his effective help in the experiments with chick

ISSN:0001-6772    

DOI:10.1111/j.1365-201X.1940.tb09442.x

出版商:Blackwell Publishing Ltd    

年代:1940

数据来源: WILEY

摘要:

Summary.Heavy water is injected into the vein of rabbits and blood samples taken at intervals from the artery. The density of the water prepared from the blood samples is determined and, from the density difference between the injected heavy water and the blood water, the extent of dilution, which the heavy water molecules experienced in the body at different times, calculated.As soon as a1/2min. after the injection, a dilution of the heavy Water by an amount of body water corresponding in volume to about that of the extracellular space of the body, is found. This very rapid rate of dilution is followed by a somewhat slower dilution process, in which presumably the cellular water participates.After the lapse of less than1/2hour, the heavy water molecules are evenly distributed over almost the total body water.After the lapse of 39 days, only about1/2of the heavy water injected is still present in the body.There is no reason to assume that the heavy water (mainly DHO) molecules show a markedly different behaviour from that of the normal water (H2O) molecules present in the body, and we have, therefore, to conclude that within about1/2min. a sufficient flow of water takes place through the capillary walls to lead to an almost perfect mixing of the blood and the interspace water. An analogous interaction between cellular and extracellular water takes less than1/2hour.

ISSN:0001-6772    

DOI:10.1111/j.1365-201X.1940.tb09443.x

出版商:Blackwell Publishing Ltd    

年代:1940

数据来源: WILEY

摘要:

Zusammenfassung.1Reine Cozymase (Adenin‐nikotinsäureamid‐dinukleotid) besitzt annähernd die gleiche blutdruckssenkende, darmhemmende und uteruserregende Wirkung wie Adenosin. Desaminocozymase ist in entsprechenden Mengen unwirksam.2Hefeadenylsäure war in unseren Versuchen weniger wirksam als Adenosin, das seinerseits schwächer als Adenosin‐triphosphorsäure wirkte.3Die Wirkung der Cozymase und der Adenylverbindungen wird mit ihrer Bedeutung für den Phosphatstoffwechsel in Bezieh

ISSN:0001-6772    

DOI:10.1111/j.1365-201X.1940.tb09444.x

出版商:Blackwell Publishing Ltd    

年代:1940

数据来源: WILEY

摘要:

Summary.A short review is given of the results of recent investigations on the chemistry of the glycoproteins and especially of the present state of the question concerning the relations between carbohydrate and protein in these substances.Hyalomucoid, synovial mucin and submaxillary mucin were investigated with the aid of electrophoresis. In approximately neutral solutions the hyalomucoid and the synovial mucin proved each to contain two electrophoretically well‐defined components. In both cases the faster migrating component was found to consist of a pure polysaccharide acid (hyaluronic acid). The slower components were simple proteins. In native synovial fluid and native vitreous body fluid the hyaluronic acid in all probability is in no way combined with protein but exists solely in the form of salts of the inorganic bases present. The glycoproteins precipitated from neutral synovial fluid or vitreous body fluid on addition of acetic acid are to be regarded as purely artificial products.Neutral solutions of submaxillary mucin were, on electrophoresis, found to contain two distinct components, both proteins. The main component, which was the faster migrating one, amounted to about 90 % of the whole and was a glycoprotein containing 27 % carbohydrate. The other component probably ought to be regarded as a contaminant.Pure water extracts from cartilage contain chondroitin sulphuric acid exclusively in the free form. This confirms the old opinion of Mörnerthat the chondroitin sulphuric acid in the cartilage is partly present as alkali salt.A chemical classification of the glycoproteins is suggest

ISSN:0001-6772    

DOI:10.1111/j.1365-201X.1940.tb09445.x

出版商:Blackwell Publishing Ltd    

年代:1940

数据来源: WILEY

摘要:

Summary.A method has been described for determining the potassium content in 0.01—0.1 c.c. of serum. The accuracy of the method is satisfactory. Added amounts of potassium are quantitatively recovered.The author's thanks are due to Dr. E. Jorpesat who's suggestion this investigation was taken u

ISSN:0001-6772    

DOI:10.1111/j.1365-201X.1940.tb09446.x

出版商:Blackwell Publishing Ltd    

年代:1940

数据来源: WILEY

摘要:

Summary.A new method for the determination of carbon monoxide in blood is described. The blood is brought into an Erlenmeyer flask, mixed with water and its carbon monoxide hemoglobin is converted into acid hematin by addition of sulfuric acid. The carbon monoxide liberated passes into another Erlenmeyer flask, which has been connected with the first one by an airtight rubber cuff and contains a known solution of palladium chloride. A corresponding quantity of the latter is reduced to metallic palladium. Excessive palladium chloride is precipitated by a known quantity of potassium iodide, and the excess of the latter is titrated after the principle of Dupré‐Winkler. The method is well fitted for micro‐anal

ISSN:0001-6772    

DOI:10.1111/j.1365-201X.1940.tb09447.x

出版商:Blackwell Publishing Ltd    

年代:1940

数据来源: WILEY

摘要:

Summary.A technic of hypophysectomy in rabbits is described. The parapharyngeal route is taken and the operation done according to the principles of hypophysectomy in rats, but modified to suit the anatomy of the rabbit. The method has great advantages. It enables direct inspection of the field of operation. The operative risk is slight. The mortality is extremely low.This work has been aided by a grant from « Stiftelsen Therese och Johan Anderssons Minne», Stockhol

ISSN:0001-6772    

DOI:10.1111/j.1365-201X.1940.tb09448.x

出版商:Blackwell Publishing Ltd    

年代:1940

数据来源: WILEY

摘要:

Summary.The combined treatment of cats with sulfapyridine and secretin is described. In conformance with other investigations, in which secretin was not used, the concentration of sulfapyridine in pancreatic juice was found to be just lower than that of blood, but an extremely high concentration of the drug was found in the bile. The drug is not diluted by the increased flow of juice either from pancreas or liver and tends to be independent of rate of flow. Blood concentration of sulfapyridine is more important. While there was no change in the percentage of free drug excreted in the pancreatic juice, 30 % of the free drug had been bound when excreted in the bile, indicating acetylation by the liver.

ISSN:0001-6772    

DOI:10.1111/j.1365-201X.1940.tb09449.x

出版商:Blackwell Publishing Ltd    

年代:1940

数据来源: WILEY

ISSN:0001-6772    

DOI:10.1111/j.1365-201X.1940.tb09450.x

出版商:Blackwell Publishing Ltd    

年代:1940

数据来源: WILEY