ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01836.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract:Polymorphic MHC class II molecules determine immune responsiveness towards pathogens and also contribute to susceptibility or resistance to a number of different autoimmune diseases, including systemic lupus erythematosus (SLE). The HLA‐DR and ‐DQ alleles of 52 patients with SLE were analyzed by serology and, for 42 patients, HLA‐DRB1, ‐B3 and DQB1 allelic polymorphism was determined by oligotyping on PCR‐amplified DNA. While we confirm the increase of DR3 (44.2% versus 16% in controls; p<0.001) reported by others, we observed a complete absence of DRwl5(2)/DR3 and DRwl5(2)/DR7 heterozygotes among Caucasian patients. Moreover HLA‐DQB1 oligotyping revealed the absence of DQB 1*0602/0201 heterozygotes in our panel of Caucasoid SLE patients. Since both DR3 and DR7 haplotypes share the same DQB 1*0201‐encoded DQβ chain, and since DRwl5 is known to be in linkage disequilibrium with DQA1*0102, it can be predicted that DQA1*0102/DQB1*0201 combinations are absent in Caucasian patients. We therefore propose that a DQA 1*0201/DQB 1*0201‐encoded HLA‐DQ transdimer formed in these heterozygotes might function as a supressor‐inducer molecule that confers re

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01837.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract:Genomic DNA of HLA class I gene segments was amplified by the polymerase chain reaction (PCR). The amplified 0.8 kb gene segment encompassed sequences corresponding to an exon 2 (α1 domain), an intron 2 and an exon 3 (α 2 domain). The PCR product was cloned in M13 and sequenced. Two previously unknown sequences were found. One of them (DAN2) had an open reading frame and intact intron splice sites, but we did not find evidence for transcription. Best homology (87.5%) was found with HLA‐BeWo C,1, a recently described HLA‐C gene. The other sequence (DAN4) is derived from a pseudogene, because the putative 3′ splice site of intron 2 was changed from AG to ATG and the sequence corresponding to exon 3 had a shift in reading frame resulting in a sto

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01838.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract:Canine pregnant sera were investigated for monocyte‐specific cytotoxic antibodies against a cell panel from 50 unrelated dogs. Contaminating DLA and DLB reactivity was removed by absorption on a random pool of purified lymphocytes. Two monocyte‐associated antigens were recognized by two independent clusters of antisera; the existence of further antigens is suggested. In preliminary segregation studies, the inheritance of these determinants in linkage with DLA could not be obser

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01839.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract:It is well established that peripheral CD8+and CD4+T cells display different requirements forin vitroactivation by mitogenic mAb. Most CD4+T cells can be activated by anti‐CD3 or mitogenetic combinations of anti‐CD2. In contrast, CD8+T cells display minimal responses to CD3 activation, and no proliferation is observed via CD2 activation. Purified peripheral blood CD8+T cells, stringently depleted of APC, have been studied for their capacity to respond to mAb directed against CD3, CD2 and CD28, used alone or in combination. It is demonstrated that proliferation can be induced by co‐stimulation of CD2 and CD28. This does not require autologous APC. CD8+T cells can also be activated by the combination of anti‐CD3 plus anti‐CD28 in the presence of APC, but only minimal cell proliferation is obtained in the absence of APC. The response via CD2 plus CD28 is IL‐2‐dependent, as demonstrated by the ability of mAb against the IL‐2 receptor to block proliferation, and is almost completely inhibited by cyclosporine A (CsA). These results suggest that the signal generated by stimulation of CD28 in combination with CD2 differs from that seen with CD28 activation combined with either PMA or CD3. Induction of IL‐2 gene activation in CD8+, CD28+peripheral T cells may therefore require additional “second signals”, which are not necessary for activation of CD4+cells. One such signal might be the interaction between CD28 a

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01840.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

摘要:

Abstract:The tissue reactivity of a monoclonal antibody raised against human granulocytes, FMC 34, was shown to overlap with antibodies including FMC 10, which is directed against the X‐hapten, and with T5A7, which is directed against lactose‐bearing antigens. Reactivity of FMC 34 on sections of human cornea was more widespread than that of either FMC 10 or T5A7. High sensitivity fluorescence testing revealed that both FMC 34 and T5A7reacted weakly with the T‐lymphocyte cell line JM, unlike the CD 15 antibody FMC 10. Competitive binding experiments showed that binding of FMC 34 to granulocytes was inhibited by preincubation with T5A7, while FMC 34 itself was able to inhibit the binding of both FMC 10 and T5A7. The binding of FMC 10 was not inhibited by preincubation of cells with T5A7. Saturation‐binding analyses revealed that each human granulocyte could bind 17.5 × 105molecyles of FMC 34, which was more than three times the number of molecules of FMC 10 which could be bound to each cell. The additional reactivity of FMC 34 appears to be due to its capacity to bind fucosylated and non‐fucosylated lactose‐bearin

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01841.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01842.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY

ISSN:0001-2815    

DOI:10.1111/j.1399-0039.1991.tb01843.x

出版商:Blackwell Publishing Ltd    

年代:1991

数据来源: WILEY